Genome Sequence of Anoxybacillus geothermalis Strain GSsed3, a Novel Thermophilic Endospore-Forming Species

Filippidou, Sevasti; Jaussi, Marion; Junier, Thomas; Wunderlin, Tina; Roussel-Delif, Ludovic; Jeanneret, Nicole; Vieth‐Hillebrand, Andrea; Vetter, Alexandra; Regenspurg, Simona; Johnson, Shannon L.; McMurry, Kim; Gleasner, Cheryl D.; Lo, Chien‐Chi; Li, Paul; Vuyisich, Momchilo; Chain, Patrick; Junier, Pilar

doi:10.1128/genomea.00575-15

Cited by 5 publications

(7 citation statements)

References 14 publications

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“…And positive result to A. mongoliensis sample Seq10 and A. kestanboliensis sample Seq18. The result of this study agree with the concept previously reported by Filippidou et al, (2015) who found urease enzyme positive for A. geothermalis Strain GSsed3.…”

Section: Discussionsupporting

confidence: 93%

Determination of extracellular hydrolytic enzyme capabilities of some Anoxybacillus isolated from hot spring environments

JAF

Erez

Ertaş

2022

Frontiers in Life Sciences and Related Technologies

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Biotechnology has grown immensely in recent years and the production of microbial enzymes was a necessary event in the industrial sectors. The enzymes and the whole cell of microorganisms have also been much used for bioremediation processes and waste management. The main industries that apply microbial enzymes are the food, textile, pharmaceutical, leather, paper, cosmetics, biomaterials, fine chemicals, energy, cellulose and detergent industries. This tool enables researchers to screen for novel bacterial strains that may have a potential enzymatic activity which have not been documented before and for looking plasmids that could serve as cloning vectors to drug resistance in thermophilic microorganism. In this study the Anoxybacillus flavithermus bacteria were used which was isolated from hot spring of Afyon city from Turkey. The bacteria were tested for the morphological and physiological aspect and analyzed by 16S rRNA comparison for identifications. In this thesis, the biochemical tests were done for identify the characteristics of the bacterium. For this purpose, starch, casein, xylanase and asparaginase hydrolysis and catalase, urease and lipase activities were examined. Also antibiotic resistant tests were performed to the isolated bacteria by using four different (chloramphenicol, ampicillin, rifamycine and erythromycin) antibiotics. The bacterium grows in nutrient agar media, and according to results; all isolated strains fermented starch as carbon and energy sources, and amylase activity was obtained at 50 °C at pH 7.0 after 24 h of the incubation. All strains were evaluated as catalase-positive, most of A. flavithermus strains were obtained as urease and caseinase positive except few strains and especially strain no. KJ095001 was founded urease and caseinase negative.

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Section: Discussionsupporting

confidence: 93%

Determination of extracellular hydrolytic enzyme capabilities of some Anoxybacillus isolated from hot spring environments

JAF

Erez

Ertaş

2022

Frontiers in Life Sciences and Related Technologies

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“…Genome sequence and annotation of this strain has been submitted to Genbank under accession number YCG00000000.1 (Filippidou et al, 2015). The DNA G+C content of GSsed3 T was slightly higher than the values reported for the reference strains (Table 1).…”

Section: Anoxybacillus Bogrovensis Dsm 17956 T (Am409184)mentioning

confidence: 92%

“…Screening of phylogenetic neighbours of strain GSsed3 T was carried out using EzTaxon-e (Kim et al, 2012), taking into account 16S rRNA gene sequences from cultured isolates. Sequences of all species of the genus Anoxybacillus along with Aeribacillus pallidus strain GS3372 (Filippidou et al, 2015), as an outgroup, were obtained and used to build phylogenetic trees using the online tools of the phylogeny.fr website (Dereeper et al, 2008). The tree topology was verified using four independent methods for the reconstruction of phylogenetic trees, neighbour-joining, maximum-likelihood, maximum-parsimony and Bayesian inference (Anisimova & Gascuel, 2006;Castresana, 2000;Dereeper et al, 2008;Elias & Lagergren, 2007;Gascuel, 1997;Goboloff et al, 2000;Guindon et al, 2010;Huelsenbeck & Ronquist, 2001;Ronquist & Huelsenbeck, 2003).…”

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confidence: 99%

Anoxybacillus geothermalis sp. nov., a facultatively anaerobic, endospore-forming bacterium isolated from mineral deposits in a geothermal station

Filippidou

Jaussi

Junier

et al. 2016

International Journal of Systematic and Evolutionary Microbiology

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A novel endospore-forming bacterium designated strain GSsed3 T was isolated from deposits clogging aboveground filters from the geothermal power platform of Groß Schönebeck in northern Germany. The novel isolate was Gram-staining-positive, facultatively anaerobic, catalase-positive and oxidase-positive. Optimum growth occurred at 60 C, 0.5 % (w/v) NaCl and pH 7-8. Analysis of the 16S rRNA gene sequence similarity indicated that strain GSsed3 T belonged to the genus Anoxybacillus, and showed 99.8 % sequence similarity to Anoxybacillus rupiensis R270 T , 98.2 % similarity to Anoxybacillus tepidamans GS5-97 T , 97.9 % similarity to Anoxybacillus voinovskiensis TH13 T , 97.7 % similarity to Anoxybacillus caldiproteolyticus DSM 15730 T and 97.6 % similarity to Anoxybacillus amylolyticus MR3C T . DNA-DNA hybridization (DDH) indicated only 16 % relatedness to Anoxybacillus rupiensis DSM 17127 T . Furthermore, DDH estimation based on genomes analysis indicated only 19.9 % overall nucleotide similarity to Anoxybacillus amylolyticus DSM 15939 T . The major respiratory menaquinone was MK-8. The polar lipid profile consisted of phosphatidylethanolamine, phosphatidylglycerol, diphosphatidylglycerol, one unknown phosphoglycolipid and one unknown phospholipid. The predominant cellular fatty acids were iso-C 15 : 0 , iso-C 17 : 0 , C 16 : 0 , iso-C 16 : 0 and anteiso-C 17 : 0 . The peptidoglycan type was A1g meso-Dpm-direct. The genomic DNA G+C content of the strain was 46.9 mol%. The phenotypic, genotypic and chemotaxonomic characterization indicated that strain GSsed3 T differs from related species of the genus. Therefore, strain GSsed3 T is considered to be a representative of a novel species of the genus Anoxybacillus, for which the name Anoxybacillus geothermalis sp. nov. is proposed. The type strain of Anoxybacillus geothermalis is GSsed3 T (=CCOS808 T =ATCC BAA2555 T ).

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“… The reference protein sequence is denoted as 100%. AB04: A. ayderensis AB04 [ 5 ]; AF/04: A. thermarum AF/04 [ 42 ]; BAA-2555: Anoxybacillus geothermalis [ 43 ]; BCO1: Anoxybacillus sp. BCO1 [ 44 ]; DSM15939: Anoxybacillus amylolyticus DSM15939 [ 42 ]; DSM27374: Anoxybacillus suryakundensis strain DSM 27374 [ 45 ]; G2: A. gonensis G2 [ 32 ]; G10: A .…”

Section: Figurementioning

confidence: 99%

Whole‐Genome Sequence Data Analysis of Anoxybacillus kamchatkensis NASTPD13 Isolated from Hot Spring of Myagdi, Nepal

Yadav

Sharma

Bhattarai

et al. 2021

BioMed Research International

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Anoxybacillus kamchatkensis NASTPD13 isolated from Paudwar hot spring of Myagdi, Nepal, upon morphological and biochemical analysis revealed to be Gram-positive, straight or slightly curved, rod-shaped, spore-forming, catalase, and oxidase-positive facultative anaerobes. It grows over a wide range of pH (5.0-11) and temperature (37-75°C), which showed growth in different reduced carbon sources such as starch raffinose, glucose, fructose, inositol, trehalose, sorbitol, mellobiose, and mannitol in aerobic conditions. Furthermore, the partial sequence obtained upon sequencing showed 99% sequence similarity in 16S rRNA gene sequence with A. kamchatkensis JW/VK-KG4 and was suggested to be Anoxybacillus kamchatkensis. Moreover, whole-genome analysis of NASTPD13 revealed 2,866,796 bp genome with a G+C content of 41.6%. Analysis of the genome revealed the presence of 102 RNA genes, which includes sequences coding for 19 rRNA and 79 tRNA genes. While the 16S rRNA gene sequence of strain NASTPD13 showed high similarity (>99%) to those of A. kamchatkensis JW/VK-KG4, RAST analysis of NASTPD13 genome suggested that A. kamchatkensis G10 is actually the closest neighbor in terms of sequence similarity. The genome annotation by RAST revealed various genes encoding glycoside hydrolases supporting that it can utilize several reduced carbon sources as observed and these genes could be important for carbohydrate-related industries. Xylanase pathway, particularly the genomic region encoding key enzymes for xylan depolymerization and xylose metabolism, further confirmed the presence of the complete gene in xylan metabolism. In addition, the complete xylose utilization gene locus analysis of NASTPD13 genome revealed all including D-xylose transport ATP-binding protein XylG and XylF, the xylose isomerase encoding gene XylA, and the gene XylB coding for a xylulokinase supported the fact that the isolate contains a complete set of genes related to xylan degradation, pentose transport, and metabolism. The results of the present study suggest that the isolated A. kamchatkensis NASTPD13 containing xylanase-producing genes could be useful in lignocellulosic biomass-utilizing industries where pentose polymers could also be utilized along with the hexose polymers.

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Genome Sequence of Anoxybacillus geothermalis Strain GSsed3, a Novel Thermophilic Endospore-Forming Species

Cited by 5 publications

References 14 publications

Determination of extracellular hydrolytic enzyme capabilities of some Anoxybacillus isolated from hot spring environments

Determination of extracellular hydrolytic enzyme capabilities of some Anoxybacillus isolated from hot spring environments

Anoxybacillus geothermalis sp. nov., a facultatively anaerobic, endospore-forming bacterium isolated from mineral deposits in a geothermal station

Whole‐Genome Sequence Data Analysis of Anoxybacillus kamchatkensis NASTPD13 Isolated from Hot Spring of Myagdi, Nepal

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