Genome Sequence of Avian Escherichia coli Strain IHIT25637, an Extraintestinal Pathogenic E. coli Strain of ST131 Encoding Colistin Resistance Determinant MCR-1

Ewers, Christa; Göttig, Stephan; Bülte, Michael; Fiedler, Sophie; Tietgen, Manuela; Leidner, Ursula; Heydel, Carsten; Bauerfeind, Rolf; Semmler, Torsten

doi:10.1128/genomea.00863-16

Cited by 19 publications

(16 citation statements)

References 15 publications

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“…In China, some farmers use colistin to increase the quality of food animals, which they raise in their backyards. More importantly, the five reported mcr-1 –harboring STs identified in the current study (156, 10, 410, 88, and 131) had been previously isolated from poultry and swine (Bai et al, 2016 ; Ewers et al, 2016 ; Falgenhauer et al, 2016 ; Yang R. S. et al, 2016 ). This included ST10, the most prevalent ST in the current study, which has also been isolated from travelers returning from India (Bernasconi et al, 2016 ) and from European animals (El Garch et al, 2016 ).…”

Section: Discussionsupporting

confidence: 50%

Molecular Epidemiology and Colistin Resistant Mechanism of mcr-Positive and mcr-Negative Clinical Isolated Escherichia coli

Luo

Zhou

et al. 2017

Front. Microbiol.

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Transmissible colistin resistance mediated by the mcr gene has been reported worldwide, but clinical isolates of mcr-negative colistin-resistant Escherichia coli are rarely reported. The aim of this study was to evaluate the mechanism of colistin resistance among mcr-positive and mcr-negative E. coli clinical isolates by performing a molecular epidemiological surveillance. For the first time ever, we show nearly the same isolation ratio for mcr-negative and mcr-positive colistin-resistant clinical isolates (47.5 and 52.5%, respectively), with no demonstrable nosocomial transmission. We provide evidence for the prevalence of the mcr-positive IncX4 plasmid and its high potential for horizontal transfer, with no obvious sequence type (ST) preference. In addition, the minimal inhibitory concentrations (MICs) of colistin of the mcr-negative E. coli isolates were obviously higher than those of mcr-positive isolates. Apart from the usually detected genes, i.e., pmrAB, phoPQ, and mgrB, other genes may be associated with the colistin resistance in mcr-negative E. coli. To the best of our knowledge, this is the first paper to report the molecular epidemiological surveillance and the proper mechanism of colistin resistance in mcr-negative E. coli clinical isolates. Together, the results show that colistin resistance was prevalent not only in the mcr-positive clinical E. coli isolates but also in the mcr-negative isolates.

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Section: Discussionsupporting

confidence: 50%

Molecular Epidemiology and Colistin Resistant Mechanism of mcr-Positive and mcr-Negative Clinical Isolated Escherichia coli

Luo

Zhou

et al. 2017

Front. Microbiol.

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“…In conclusion, our report on the detection of mcr-1 in APEC associated with colibacillosis in production poultry is relatively new, and we could find only three other studies by Perreten and colleagues [61] and Yang et al [30] reporting mcr-1 in APEC in South Africa and China and a genome announcement of an avian ExPEC from Germany [31]. Regardless, our study would appear to be one of the first identifying mcr-1 in APEC from China and Egypt of earlier dates than previously reported.…”

Section: Discussionmentioning

confidence: 87%

mcr-1 identified in Avian Pathogenic Escherichia coli (APEC)

et al. 2017

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Antimicrobial resistance associated with colistin has emerged as a significant concern worldwide threatening the use of one of the most important antimicrobials for treating human disease.Here, we examined a collection (n = 980) of Avian Pathogenic Escherichia coli (APEC) isolated from poultry with colibacillosis from the US and internationally for the presence of mcr-1 and mcr-2, genes known to encode colistin resistance. Included in the analysis was an additional set of avian fecal E. coli (AFEC) (n = 220) isolates from healthy birds for comparative analysis. The mcr-1 gene was detected in a total of 12 isolates recovered from diseased production birds from China and Egypt. No mcr genes were detected in the healthy fecal isolates. The full mcr-1 gene from positive isolates was sequenced using specifically designed primers and were compared with sequences currently described in NCBI. mcr-1 positive isolates were also assessed for phenotypic colistin resistance and extended spectrum beta lactam phenotypes and genotypes. This study has identified mcr-1 in APEC isolates dating back to at least 2010 and suggests that animal husbandry practices could result in a potential source of resistance to the human food chain in countries where application of colistin in animal health is practiced.

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“…The high abundance of ST 95 and ST 131 in this study is not unexpected. Both STs are commonly associated with disease and outbreaks in poultry where they may cause considerable economic loss and reduced animal welfare due to increased morbidity and mortality of the affected flocks [5,[25][26][27]. It may also be suggested that these STs have a zoonotic potential as they are also commonly found among human infections, the two STs are among the most common STs in bloodstream-and urinary tract infections [28][29][30][31].…”

Section: Discussionmentioning

confidence: 99%

Whole genome sequence comparison of avian pathogenic Escherichia coli from acute and chronic salpingitis of egg laying hens

et al. 2020

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Background: Infection in the oviduct (salpingitis) is the most common bacterial infection in egg laying hens and is mainly caused by Escherichia coli. The disease is responsible for decreased animal welfare, considerable economic loss as well as a risk of horizontal and vertical transmission of pathogenic E. coli. The outcome of salpingitis may be either acute or chronic. It has not yet been clarified whether the pathological manifestation is a result of the characteristics of the E. coli or whether the manifestation is associated with host factors such as host immunity. Results: From the core-and accessory genome analysis and comparison of 62 E. coli no genetic markers were found to be associated to either acute or chronic infection. Twenty of the 62 genomes harboured at least one antimicrobial resistance gene with resistance against sulfonamides being the most common. The increased serum survival and iron chelating genes iss and iroN were highly prevalent in genomes from both acute and chronic salpingitis. Conclusion: Our analysis revealed that no genetic markers could differentiate the E. coli isolated from acute versus chronic salpingitis in egg laying hens. The difference in pathological outcome may be related to other factors such as immunological status, genetics and health of the host. These data indicate that salpingitis is another manifestation of colibacillosis.

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Genome Sequence of Avian Escherichia coli Strain IHIT25637, an Extraintestinal Pathogenic E. coli Strain of ST131 Encoding Colistin Resistance Determinant MCR-1

Cited by 19 publications

References 15 publications

Molecular Epidemiology and Colistin Resistant Mechanism of mcr-Positive and mcr-Negative Clinical Isolated Escherichia coli

Molecular Epidemiology and Colistin Resistant Mechanism of mcr-Positive and mcr-Negative Clinical Isolated Escherichia coli

mcr-1 identified in Avian Pathogenic Escherichia coli (APEC)

Whole genome sequence comparison of avian pathogenic Escherichia coli from acute and chronic salpingitis of egg laying hens

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