Genome Sequence of Bacillus velezensis P1, a Strain Isolated from a Biofilm Captured on a Pig Farm Building

Guéneau, Virgile; Piard, Jean-Christophe; Frayssinet, Bastien; Loux, Valentin; Chiapello, Hélène; Plateau-Gonthier, Julia; Castex, Mathieu; Briandet, Romain

doi:10.1128/mra.01219-21

Cited by 2 publications

(1 citation statement)

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“…Modeling the interactions and testing the ecological theories within these positive bacterial consortia in simplified communities appear to be a promising step to improve the product composition (De Roy et al, 2014 ). In addition, the study of bacteria naturally present in farms would allow new selection criteria of strains (phenotypes and genomes), already identified as capable of living in these environments (Guéneau et al, 2022a ).…”

Section: Discussionmentioning

confidence: 99%

Positive biofilms to control surface-associated microbial communities in a broiler chicken production system - a field study

et al. 2022

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In the One Health concept, the use of beneficial bacteria to form positive biofilms that prevent the settlement of undesirable bacteria is a promising solution to limit the use of antimicrobials on farms. However, there is a lack of field studies reporting the onset of these beneficial bacteria after application and the effects on autochthonous surface microbiota. In the study reported here, the inner surfaces of commercial broiler chicken houses were treated or not with a bacterial consortium composed of Bacillus spp. and Pediococcus spp. strains, able to form covering biofilms in different laboratory models. Preinstalled coupons were sampled over time to capture microbial biofilm dynamics on-farm surfaces. The results showed that the bacterial consortium can establish on the farm surfaces, modulate microbial communities, and limit the implantation of Enterobacteriaceae and Enterococcaceae, two families containing potential pathogens.

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Section: Discussionmentioning

confidence: 99%

Positive biofilms to control surface-associated microbial communities in a broiler chicken production system - a field study

et al. 2022

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Insights into the genomic and phenotypic characteristics of Bacillus spp. strains isolated from biofilms in broiler farms

Guéneau,

Jiménez,

Castex

et al. 2024

Appl Environ Microbiol

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The characterization of surface microbiota living in biofilms within livestock buildings has been relatively unexplored, despite its potential impact on animal health. To enhance our understanding of these microbial communities, we characterized 11 spore-forming strains isolated from two commercial broiler chicken farms. Sequencing of the strains revealed them to belong to three species Bacillus velezensis , Bacillus subtilis , and Bacillus licheniformis . Genomic analysis revealed the presence of antimicrobial resistance genes and genes associated with antimicrobial secretion specific to each species. We conducted a comprehensive characterization of the biofilm formed by these strains under various conditions, and we revealed significant structural heterogeneity across the different strains. A macro-colony interaction model was employed to assess the compatibility of these strains to coexist in mixed biofilms. We identified highly competitive B. velezensis strains, which cannot coexist with other Bacillus spp. Using confocal laser scanning microscopy along with a specific dye for extracellular DNA, we uncovered the importance of extracellular DNA for the formation of B. licheniformis biofilms. Altogether, the results highlight the heterogeneity in both genome and biofilm structure among Bacillus spp. isolated from biofilms present within livestock buildings. IMPORTANCE Little is known about the microbial communities that develop on farms in direct contact with animals. Nonpathogenic strains of Bacillus velezensis , Bacillus subtilis , and Bacillus licheniformis were found in biofilm samples collected from surfaces in contact with animals. Significant genetic and phenotypic diversity was described among these Bacillus strains. The strains do not possess mobile antibiotic resistance genes in their genomes and have a strong capacity to form structured biofilms. Among these species, B. velezensis was noted for its high competitiveness compared with the other Bacillus spp. Additionally, the importance of extracellular DNA in the formation of B. licheniformis biofilms was observed. These findings provide insights for the management of these surface microbiota that can influence animal health, such as the use of competitive strains to minimize the establishment of undesirable bacteria or enzymes capable of specifically deconstructing biofilms.

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Genome Sequence of Bacillus velezensis P1, a Strain Isolated from a Biofilm Captured on a Pig Farm Building

Abstract: The genome of the Bacillus velezensis P1 strain isolated from a biofilm on the wall of a pig farm was sequenced. The strain harbors many surface colonization genes involved in surfactant, matrix, and antibacterial synthesis.

Cited by 2 publications

References 9 publications

Positive biofilms to control surface-associated microbial communities in a broiler chicken production system - a field study

Positive biofilms to control surface-associated microbial communities in a broiler chicken production system - a field study

Insights into the genomic and phenotypic characteristics of Bacillus spp. strains isolated from biofilms in broiler farms

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