Genome Sequence of Bellavista Virus, a Novel Orthobunyavirus Isolated from a Pool of Mosquitoes Captured near Iquitos, Peru

Hang, Jun; Yang, Yu; Kuschner, Robert A.; Evangelista, Julio; Astete, Helvio; Halsey, Eric S.; Kochel, Tadeusz J.; Forshey, Brett M.

doi:10.1128/genomea.01262-16

Cited by 5 publications

(1 citation statement)

References 15 publications

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“…Mosquitoes of the Culicidae family represent a threat to public global health, especially in tropical and subtropical regions, where they participate as the main vectors in the transmission of infectious agents that affect humans and animals. Of the circa 3700 species of mosquitoes worldwide, 1069 species are registered in the neotropical region (Wilkerson et al, 2021), of which in Peru, 182 species have been o cially reported; of these, 44 belong to the genus Culex Linnaeus, 1758 (Ayala et al, 2020(Ayala et al, , 2021) and many of these are considered arbovirus vectors, especially the species of the subgenus Melanoconion (Turell et al, 2005;Yanoviak et al, 2005;Turell et al, 2006Turell et al, , 2008Evangelista et al, 2013;Hang et al, 2016;Treangen et al, 2016;Turell et al, 2021). In Peru, the arboviruses transmitted by Culex spp.…”

Section: Introductionmentioning

confidence: 99%

First report of Culex (Culex) camposi Dyar, 1925 and Culex (Carrollia) bonnei Dyar, 1921 via male genitalia and CoxI barcoding sequence analyses in Huanuco, Peru

Sabino,

Marquez-Ocaña,

Otiniano-Moreno

et al. 2023

Preprint

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Mosquitoes (Diptera: Culicidae) pose a significant threat to public health worldwide, especially in tropical and subtropical regions, where they act as primary vectors in transmission of infectious agents. In Peru, where 182 culicid species have been identified, several species of the genus Culex are known to transmit arboviruses. However, knowledge of the diversity and distribution of mosquitoes remains limited, with many studies focusing on specific regions only. This study reports the presence of two mosquito species in Peru: ex-larvae Culex (Culex) camposi Dyar, 1925 and Cx. (Carrollia) bonnei Dyar, 1921, identified through larval collections and molecular analyses. In total, 10 mosquitoes (8 males and 2 females) were analyzed, the dissection of the genitalia of the 8 male specimens allowed the identification of the species Cx. camposi and Cx. bonnei. A total of 10 partial sequences of the CoxI gene corresponding to these two species were obtained. Phylogenetic analysis revealed that the sequences of Cx. camposi formed the same monophyletic clade with sequences from species of the Coronator complex, in addition to presenting a divergence of less than 2%, while Cx. bonnei, formed in a monophyletic clade, with > 2% divergence with sequences from other species corresponding to the subgenus Carrollia. This study underscores the importance of continued efforts to study the diversity and distribution of mosquitoes in Peru, including their potential role as vectors of human pathogens, to underpin effective disease control and prevention strategies.

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Section: Introductionmentioning

confidence: 99%

First report of Culex (Culex) camposi Dyar, 1925 and Culex (Carrollia) bonnei Dyar, 1921 via male genitalia and CoxI barcoding sequence analyses in Huanuco, Peru

Sabino,

Marquez-Ocaña,

Otiniano-Moreno

et al. 2023

Preprint

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The discovery and global distribution of novel mosquito‐associated viruses in the last decade (2007‐2017)

Atoni

Zhao

Karungu

et al. 2019

Reviews in Medical Virology

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Summary In the last decade, virus hunting and discovery has gained pace. This achievement has been driven by three major factors: (a) advancements in sequencing technologies, (b) scaled‐up routine arbovirus surveillance strategies, and (c) the “hunt” for emerging pathogens and novel viruses. Many novel viruses have been discovered from a myriad of hosts, vectors, and environmental samples. To help promote understanding of the global diversity and distribution of mosquito‐associated viruses and facilitate future studies, we review mosquito‐associated viruses discovered between years 2007 and 2017, across the world. In the analyzed period, novel mosquito‐associated viruses belonging to 25 families and a general group of unclassified viruses were categorized. The top three discovered novel mosquito‐associated viruses belonged to families Flaviviridae (n=32), Rhabdoviridae (n=16), and Peribunyaviridae (n=14). Also, 67 unclassified viruses were reported. Majority of these novel viruses were identified from Culex spp, Anopheles spp, Aedes spp, and Mansonia spp mosquitoes, respectively. Notably, the number of these discovered novels is not representative of intercontinental virus diversity but rather is influenced by the number of studies done in the study period. Some of these newly discovered mosquito‐associated viruses have medical significance, either directly or indirectly. For instance, in the study period, 14 novel mosquito‐borne viruses that infect mammalian cells in vitro were reported. These viruses pose a danger to the global health security on emerging viral diseases. On the other hand, some of the newly discovered insect specific viruses described herein have potential application as future biocontrol and vaccine agents against known pathogenic arboviruses. Overall, this review outlines the crucial role played by mosquitoes as viral vectors in the global virosphere.

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Description of New Morphological Variation of Culex (Culex) coronator Dyar and Knab, 1906 and First Report of Culex (Carrollia) bonnei Dyar, 1921 Found in the Central Region of Peru

Montalvo-Sabino,

Marquez-Ocaña,

Otiniano-Moreno

et al. 2024

Neotrop Entomol

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Mosquitoes (Diptera: Culicidae) pose a significant threat to public health worldwide, especially in tropical and subtropical regions, where they act as primary vectors in transmission of infectious agents. In Peru, 182 culicid species have been identified and several species of the genus Culex are known to transmit arboviruses. However, knowledge of mosquito diversity and distribution remains limited, with many studies focusing on specific regions only. Here, we describe a new morphological variation of Cx. (Culex) coronator Dyar and Knab, 1906, and report the presence of Culex (Carrollia) bonnei Dyar, 1921 in the central region of Peru, Huanuco. Specimens were obtained through larvae collections and identified through morphologic characterization, including dissection of male genitalia, and molecular analyses. In total, 17 mosquitoes were analyzed, and the genitalia of the male specimens allowed the identification of Cx. coronator and Cx. bonnei. Partial sequences of the CoxI gene corresponding to these two species were obtained (N = 10). Phylogenetic analysis revealed that the sequences of Cx. coronator grouped in a monophyletic clade with sequences ascribed to other species corresponding to the subgenus Carrollia, while Cx. bonnei specimens formed a monophyletic clade with homologous sequences from GenBank. This study underscores the importance of continued efforts to study the diversity and distribution of mosquitoes in Peru, including their potential role as vectors of human pathogens, to underpin effective disease control and prevention strategies, highlighting the importance of a complemented morphological and molecular analysis.

show abstract

Genome Sequence of Bellavista Virus, a Novel Orthobunyavirus Isolated from a Pool of Mosquitoes Captured near Iquitos, Peru

Cited by 5 publications

References 15 publications

First report of Culex (Culex) camposi Dyar, 1925 and Culex (Carrollia) bonnei Dyar, 1921 via male genitalia and CoxI barcoding sequence analyses in Huanuco, Peru

First report of Culex (Culex) camposi Dyar, 1925 and Culex (Carrollia) bonnei Dyar, 1921 via male genitalia and CoxI barcoding sequence analyses in Huanuco, Peru

The discovery and global distribution of novel mosquito‐associated viruses in the last decade (2007‐2017)

Description of New Morphological Variation of Culex (Culex) coronator Dyar and Knab, 1906 and First Report of Culex (Carrollia) bonnei Dyar, 1921 Found in the Central Region of Peru

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