Genome Sequence of Brevibacillus brevis HK544, an Antimicrobial Bacterium Isolated from Soil in Daejeon, South Korea

Kim, Bomin; Kim, Yeong Seok; Han, Jae Woo; Choi, Gyung Ja; Kim, Hun

doi:10.1128/mra.00417-21

Cited by 6 publications

(1 citation statement)

References 57 publications

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“…The HK544 strain was previously identified as a B. brevis at the genome level ( 20 ). Prior to the chemical identification of the respirator inhibitor from B. brevis HK544 CF, we analyzed and compared the secondary metabolites derived from the HK544 genome with several Brevibacillus and Bacillus species ( 21 , 22 ).…”

Section: Resultsmentioning

confidence: 99%

Edeine B ₁ produced by Brevibacillus brevis reduces the virulence of a plant pathogenic fungus by inhibiting mitochondrial respiration

Kim,

Nguyen,

Park

et al. 2024

mBio

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Plant pathogenic fungi cause serious diseases, which result in the loss of crop yields and reduce the quality of crops worldwide. To counteract the escalating risks of chemical fungicides, interest in biological control agents to manage plant diseases has significantly increased. In this study, we comprehensively screened microbial culture filtrates using a yeast screening system to find microbes exhibiting respiratory inhibition activity. Consequently, we found a soil-borne microbe Brevibacillus brevis HK544 strain exhibiting a respiration inhibitory activity and identified edeine B 1 (EB 1 ) from the culture filtrate of HK544 as the active compound of the respiration inhibition activity. Furthermore, against a plant pathogenic fungus Fusarium graminearum , our results showed that EB 1 has effects on multiple aspects of respiration with the downregulation of most of the mitochondrial-related genes based on transcriptome analysis, differential EB 1 -sensitivity from targeted mutagenesis, and the synergistic effects of EB 1 with electron transport chain complex inhibitors. With the promising plant disease control efficacy of B. brevis HK544 producing EB 1 , our results suggest that B. brevis HK544 has potential as a biocontrol agent for Fusarium head blight. IMPORTANCE As a necrotrophic fungus, Fusarium graminearum is a highly destructive pathogen causing severe diseases in cereal crops and mycotoxin contamination in grains. Although chemical control is considered the primary approach to control plant disease caused by F. graminearum , fungicide-resistant strains have been detected in the field after long-term continuous application of fungicides. Moreover, applying chemical fungicides that trigger mycotoxin biosynthesis is a great concern for many researchers. Biocontrol of Fusarium head blight (FHB) by biological control agents (BCAs) represents an alternative approach and could be used as part of the integrated management of FHB and mycotoxin production. The most extensive studies on bacterial BCAs-fungal communications in agroecosystems have focused on antibiosis. Although many BCAs in agricultural ecology have already been used for fungal disease control, the molecular mechanisms of antibiotics produced by BCAs remain to be elucidated. Here, we found a potential BCA ( Brevibacillus brevis HK544) with a strong antifungal activity based on the respiration inhibition activity with its active compound edeine B 1 (EB 1 ). Furthermore, our results showed that EB 1 secreted by HK544 suppresses the expression of the mitochondria-related genes of F. graminearum , subsequently suppressing fungal development and the virulence of F. graminearum . In addition, EB 1 exhibited a synergism with complex I inhibitors such as rotenone and fenazaquin. Our work extends our understanding of how B. brevis HK544 exhibits antifungal activity and suggests that the B. brevis HK544 strain could be a valuable source for developing new crop protectants to control F. graminearum .

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Section: Resultsmentioning

confidence: 99%

Edeine B ₁ produced by Brevibacillus brevis reduces the virulence of a plant pathogenic fungus by inhibiting mitochondrial respiration

Kim,

Nguyen,

Park

et al. 2024

mBio

Self Cite

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Complete genome sequence analysis and Pks genes identification of Brevibacillus brevis FJAT-0809-GLX with a broad inhibitory spectrum against phytopathogens

Che,

Lai,

Lai

et al. 2024

World J Microbiol Biotechnol

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Biocontrol potential of Bevibacillus brevis HK544 for fungal plant diseases

Kim,

Han

et al. 2024

Biological Control

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Genome Sequence of Brevibacillus brevis HK544, an Antimicrobial Bacterium Isolated from Soil in Daejeon, South Korea

Cited by 6 publications

References 57 publications

Edeine B ₁ produced by Brevibacillus brevis reduces the virulence of a plant pathogenic fungus by inhibiting mitochondrial respiration

Edeine B ₁ produced by Brevibacillus brevis reduces the virulence of a plant pathogenic fungus by inhibiting mitochondrial respiration

Complete genome sequence analysis and Pks genes identification of Brevibacillus brevis FJAT-0809-GLX with a broad inhibitory spectrum against phytopathogens

Biocontrol potential of Bevibacillus brevis HK544 for fungal plant diseases

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Genome Sequence of Brevibacillus brevis HK544, an Antimicrobial Bacterium Isolated from Soil in Daejeon, South Korea

Cited by 6 publications

References 57 publications

Edeine B 1 produced by Brevibacillus brevis reduces the virulence of a plant pathogenic fungus by inhibiting mitochondrial respiration

Edeine B 1 produced by Brevibacillus brevis reduces the virulence of a plant pathogenic fungus by inhibiting mitochondrial respiration

Complete genome sequence analysis and Pks genes identification of Brevibacillus brevis FJAT-0809-GLX with a broad inhibitory spectrum against phytopathogens

Biocontrol potential of Bevibacillus brevis HK544 for fungal plant diseases

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Edeine B ₁ produced by Brevibacillus brevis reduces the virulence of a plant pathogenic fungus by inhibiting mitochondrial respiration

Edeine B ₁ produced by Brevibacillus brevis reduces the virulence of a plant pathogenic fungus by inhibiting mitochondrial respiration