Genome Sequence of Aedes aegypti , a Major Arbovirus Vector

Abstract

We present a draft sequence of the genome of Aedes aegypti, the primary vector for yellow fever and dengue fever, which at ~1.38 Gbp is ~5-fold larger in size than the genome of the malaria vector, Anopheles gambiae. Nearly 50% of the Aedes aegypti genome consists of transposable elements. These contribute to a ~4-6 fold increase in average gene length and the size of intergenic regions relative to Anopheles gambiae and Drosophila melanogaster. Nevertheless, chromosomal synteny is generally maintained between all three insects although conservation of orthologous gene order is higher (~2-fold) between the mosquito species than between either of them and fruit fly. Three methods have provided transcriptional evidence for 80% of the 15,419 predicted protein coding genes in Aedes aegypti. An increase in genes encoding odorant binding, cytochrome P450 and cuticle domains relative to Anopheles gambiae suggests that members of these protein families underpin some of the biological differences between them.

Identity and transfer of male reproductive gland proteins of the dengue vector mosquito, Aedes aegypti: Potential tools for control of female feeding and reproduction

“…This discrepancy could result from a mis-annotation of these two genes, especially given the large intron size frequently seen in Ae. aegypti (Nene et al, 2007). Although we detected 15 and 2 proteins in the 30 and 60 kDa range, respectively, which are reported to be the size of the main protein bands in the partially-purified matrone (Fuchs et al, 1969), we cannot be certain that any correspond to matrone's active fraction since the active component is unknown.…”

Quantitative trait loci determining autogeny and body size in the Asian tiger mosquito (Aedes albopictus)

“…Mosquito genomics has achieved a level wherein the molecular genetic basis for complex traits such as autogeny and wing length can be elucidated. Complete annotated genome sequences are available for Aedes aegypti (Nene et al, 2007) and Anopheles gambiae (Holt et al, 2002), and will soon be available for Culex pipiens quinquefasciatus (http://cpipiens.vectorbase.org/ index.php). Because of its importance as a disease vector, it seems likely that the genome sequence for Ae.…”

Insights into the transcriptome of oenocytes from Aedes aegypti pupae

“…aegypti, approximately 8% of these proteins are members of the P450 family. These data represented an expansion of P450 gene expression in comparison to Drosophila and Anopheles that could potentially enable a more elaborate detoxifying system and contribute to the overall robustness associated with Aedes in comparison to other species (Nene et al 2007). An expansion of P450s gene families in the recent Cux.…”