Genome Sequence of “
            <i>Candidatus</i>
            Viridilinea halotolerans” Chok-6, Isolated from a Saline Sulfide-Rich Spring

Grouzdev, Denis S.; Burganskaya, Ekaterina I; Krutkina, Maria S.; Сухачева, М. В.; Горленко, В. М.

doi:10.1128/mra.01614-18

Cited by 13 publications

(1 citation statement)

References 19 publications

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“…trichoides DG-6 is currently the only representative of the order Chloroflexales whose capacity for nitrogen fixation has been demonstrated using genome data as well as experimentally. However, the genome data indicate that the recently isolated AFPB species "Candidatus Oscillochloris kuznetsovii" and "Candidatus Oscillochloris fontis," "Candidatus Chloroploca asiatica" and "Candidatus Viridilinea mediisalina" may be capable of diazotrophy, due to the presence of the nitrogenase gene cluster (Grouzdev et al, 2018(Grouzdev et al, , 2019Gaisin et al, 2019aGaisin et al, , 2019bGaisin et al, , 2020 that is homologous to the nifHBDK cluster of the strain Osc. trichoides GD-6.…”

Section: Potential Functional Characteristics Of Nitrogen Metabolism Of the Cultivable Afpb Strains Of The Order Cloroflexales Based On Cmentioning

confidence: 99%

Nitrogen Metabolism of an Anoxygenic Filamentous Phototrophic Bacterium Oscillocholris trichoides Strain DG-6

et al. 2021

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Abstract— The possible nitrogen sources for Osc. trichoides DG6, a typical strain of the Oscillochloridaceae family, are ammonium, N2, glutamate, asparagine, glycine, and glutamine. The assimilation of molecular nitrogen occurs with the participation of nitrogenase, the structural gene of which, nifH, is located in the gene cluster which also includes the genes of the nifD and nifK nitrogenase subunits and the auxiliary nifB gene. Considering that nifHBDK clusters have been also annotated in the genomes of other members of the Oscillochloridaceae family, including uncultured and candidate taxa, it can be assumed that the ability to fix nitrogen is a property immanent for this entire family. The pathways for assimilating ammonium in the cells grown using different nitrogen sources may differ. Osc. trichoides DG6 growing in a medium containing ammonium assimilated it with the participation of glutamate dehydrogenase, which is determined by a single gene. The expression product of this gene has dual functionality and can be used to implement the reaction with both NAD and NADP. With the growth of Osc. trichoides DG6 on a medium with glutamate as the only nitrogen source all the enzymes necessary for the implementation of the GS‑GOGAT pathway were found in the cells. However, for the glutamine synthetase reaction, ammonium, which was absent in the growth medium, was required. The source of ammonium may be glutamate metabolized through glutamate dehydrogenase.

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Section: Potential Functional Characteristics Of Nitrogen Metabolism Of the Cultivable Afpb Strains Of The Order Cloroflexales Based On Cmentioning

confidence: 99%