Genome Sequence of
            <i>Vibrio</i>
            VPAP30, Isolated from an Episode of Massive Mortality of Reared Larvae of the Scallop
            <i>Argopecten purpuratus</i>

Rojas, Rodrigo; Miranda, Claudio D.; Romero, Jaime; Asenjo, Freddy; Valderrama, Katherinne; Segovia, Cristopher; Ugalde, Juan A.; Santander, Javier

doi:10.1128/genomea.00745-15

Cited by 5 publications

(4 citation statements)

References 9 publications

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“…Although various V. coralliilyticus strains were previously miss-identified as V. tubiashii , sequence analysis performed on the VPAP30 strain clearly differentiated this strain to the V. coralliilyticus species, despite the apparent carriage of a metalloprotease-encoding gene similar to the detected in V. coralliilyticus ( Rojas et al, 2015b ). Furthermore, many studies reported V. coralliilyticus as an opportunistic pathogen against larvae of various mollusc species, including Greenshell mussel Perna canaliculus , great scallop Pecten maximus and oysters Crassostrea gigas, Crassostrea virginica , and Ostrea edulis ( Kesarcodi-Watson et al, 2009 , 2012 ; Genard et al, 2013 ; Richards et al, 2015 ), prompting the necessity for adequate differentiation between V. tubiashii and V. coralliilyticus species when they are involved in mollusc larval pathologies.…”

Section: Discussionmentioning

confidence: 94%

“…It must be noted that a metalloprotease encoding gene similar to vtpA was detected in the genome of V . tubiashii VPAP30 ( Rojas et al, 2015b ). Moreover, Hasegawa et al (2008) demonstrated that metalloprotease inhibitors severely reduce the toxicity of ECPs produced by two strains recently identified as V. coralliilyticus ( Wilson et al, 2013 ; Richards et al, 2014 ), but previously miss-identified as V. tubiashii , on Pacific oyster larvae.…”

Section: Discussionmentioning

confidence: 99%

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First Report of Vibrio tubiashii Associated with a Massive Larval Mortality Event in a Commercial Hatchery of Scallop Argopecten purpuratus in Chile

et al. 2016

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The VPAP30 strain was isolated as the highly predominant bacteria from an episode of massive larval mortality occurring in a commercial culture of the Chilean scallop Argopecten purpuratus. The main aims of this study were, to characterize and identify the pathogenic strain using biochemical and molecular methods to demonstrate its pathogenic activity on scallop larvae, to characterize its pathogenic properties and to describe the chronology of this pathology. The pathogenic strain was identified as Vibrio tubiashii based on its phenotypic properties and the sequence analysis of its 16S rRNA and housekeeping genes (ftsZ, gapA, gyrB, mreB, pyrH, recA, rpoA and topA). When triplicate cultures of healthy 10–day–old scallop larvae were challenged with 1 × 105 colony forming units (CFU) mL-1 of the VPAP30 strain, percentages of larval survival of 78.87 ± 3.33%, 34.32 ± 4.94%, and 0% were observed at 12, 24, and 36 h, respectively; whereas uninfected larval cultures showed survival rates of 97.4 ± 1.24% after of 48 h. Clinical symptoms exhibited by the scallop larvae infected with the VPAP30 strain include the accumulation of bacteria around the scallop larvae, velum disruption and necrosis of digestive gland. The 50% lethal dose (LD50) of VPAP30 strain at 24 and 48 h was 1.3 × 104 and 1.2 × 103 CFU mL-1, respectively. The invasive pathogenic activity of the VPAP30 strain was investigated with staining of the bacterial pathogen with 5-DTAF and analyzing bacterial invasion using epifluorescence, and a complete bacterial dissemination inside the larvae at 24 h post-infection was observed. When scallop larvae were inoculated with cell-free extracellular products (ECPs) of VPAP30, the larval survival rate was 59.5 ± 1.66%, significantly (P < 0.001) lower than the control group (97.4 ± 1.20%) whereas larvae treated with heat-treated ECPs exhibited a survival rate of 61.6 ± 1.84% after 48 h of exposure. This is the first report of the isolation of V. tubiashii from the diseased larvae of the scallop A. purpuratus, occurring in a commercial culture in Chile, and it was demonstrated that the VPAP30 strain exhibits high pathogenic activity on scallop larvae, mediated both by bacterial invasion and the production of toxigenic heat-stable compounds.

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Section: Discussionmentioning

confidence: 94%

Section: Discussionmentioning

confidence: 99%

First Report of Vibrio tubiashii Associated with a Massive Larval Mortality Event in a Commercial Hatchery of Scallop Argopecten purpuratus in Chile

et al. 2016

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“…It must be noted that a metalloprotease encoding gene similar to vtpA was detected in the genome of this pathogenic strain (formerly V . tubiashii VPAP30) (Rojas et al, 2015b). Moreover, Hasegawa et al (2008) demonstrated that metalloprotease inhibitors severely reduce the toxicity of ECPs produced by 2 strains recently identified as V. coralliilyticus (Wilson et al, 2013; Richards et al, 2014), but previously miss-identified as V. tubiashii , on Pacific oyster larvae.…”

Section: Discussionmentioning

confidence: 99%

Isolation and Pathogenic Characterization of Vibrio bivalvicida Associated With a Massive Larval Mortality Event in a Commercial Hatchery of Scallop Argopecten purpuratus in Chile

et al. 2019

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The VPAP30 strain was isolated as the highly predominant bacteria from an episode of massive larval mortality occurring in a commercial culture of the Chilean scallop Argopecten purpuratus . The main aims of this study were, to characterize and identify the pathogenic strain using biochemical and molecular methods, to demonstrate its pathogenic activity on scallop larvae, to characterize its pathogenic properties and to describe the chronology of the pathology. The pathogenic strain was identified as Vibrio bivalvicida based on its phenotypic properties, the multilocus sequence analysis (MLSA) of eight housekeeping genes ( fts Z, gap A, gyr B, mre B, pyr H, rec A, rpo A, and top A) and different in silico genome-to-genome comparisons. When triplicate cultures of healthy 10 days old scallop larvae were challenged with 1 × 10 5 colony forming units (CFU) mL -1 of the VPAP30 strain, percentages of larval survival of 78.9 ± 3.3%, 34.3 ± 4.9%, and 0% were observed at 12, 2,4 and 36 h, respectively, whereas uninfected larval cultures showed survival rates of 97.4 ± 1.2% after of 48 h. Clinical symptoms exhibited by the scallop larvae infected with the VPAP30 strain include the accumulation of bacteria around the scallop larvae, velum disruption and necrosis of digestive gland. The 50% lethal dose (LD 50 ) of VPAP30 strain at 24 and 48 h was 1.3 × 10 4 and 1.2 × 10 3 CFU mL -1 , respectively. The invasive pathogenic activity of the VPAP30 strain was investigated with staining of the bacterial pathogen with 5-DTAF and analyzing bacterial invasion using epifluorescence, and a complete bacterial dissemination inside the larvae at 24 h post-infection was observed. When scallop larvae were inoculated with cell-free extracellular products (ECPs) of VPAP30, the larval survival rate was 59.5 ± 1.7%, significantly ( P < 0.001) lower than the control group (97.4 ± 1.2%) whereas larvae treated with heat-treated ECPs exhibited a survival rate of 61.6 ± 1.8% after 48 h of exposure. V. bivalvicida VPAP30 exhibits high pathogenic activity on scallop larvae, mediated both by bacterial invasion and the production of toxigenic heat-stable compounds. This report constitutes the first isolation of V. bivalvicida out of Europe and extends the host range of this species, having demonstrated its pathogenic activity on the Chilean scallop larvae ( A. purpuratus ). These results supporting the pathogenic potential of V. bivalvicida to kill the larvae of a broad range of bivalve species reared in hatcheries located in the Atlantic and the Paci...

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“…In other cases, recurrent mortality episodes are described as a result of virus or Vibrio-like bacteria infecting bivalve hatcheries and nurseries (Renault and Arzul, 2001;Dubert et al, 2015;Rojas et al, 2015) and reducing commercial production by approximately 40% as reported for French farming facilities of Pacific oyster (Crassostrea gigas) since 2008 (Pernet et al, 2014).…”

Section: Introductionmentioning

confidence: 99%

Adult spawning and early larval development of the endangered bivalve Pinna nobilis

Trigos¹,

Vicente

Prado

et al. 2018

Aquaculture

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The development of aquaculture activities has posed an alternative solution for the preservation of some overexploited shellfish fisheries worldwide. In the same way, endemic Mediterranean bivalves such as Pinna nobilis, highly threatened by habitat loss and coastal pollution, could found in aquaculture a solution for preserving the continuity of the species. Given the endangered status of the species, the biological and ecological processes regulating natural populations have been well studied, but there are still important knowledge gaps preventing the development of viable artificial cultures. This study describes for the first time the larval development of P. nobilis (from fertilization until pediveliger larval stages) in captivity conditions. Moreover, different rearing tanks of 5, 16 and 80 L, larvae density from 1 to 600 larvae mL −1 , light conditions, food doses, were tested in order to establish the bases for the optimal rearing of the species and provide a source of individuals for restoring field populations. Results showed that 16 L tanks with a concentration of 2 larvae mL −1 , constant temperature of 21 °C, 12/12 h photoperiod and fed with an "optimal" mixture of 25 cells per μL of Chaetoceros calcitrans + 33.3 cells per μL ofPavlova lutheri + 100 cells per μL of Isochrysis galbana" appear to be the best conditions to rear larvae of P. nobilis. Different captivity conditions such as lower or higher tank volume, larvae density, or food doses; light privation did not report better results for larval development.

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Genome Sequence of Vibrio VPAP30, Isolated from an Episode of Massive Mortality of Reared Larvae of the Scallop Argopecten purpuratus

Cited by 5 publications

References 9 publications

First Report of Vibrio tubiashii Associated with a Massive Larval Mortality Event in a Commercial Hatchery of Scallop Argopecten purpuratus in Chile

First Report of Vibrio tubiashii Associated with a Massive Larval Mortality Event in a Commercial Hatchery of Scallop Argopecten purpuratus in Chile

Isolation and Pathogenic Characterization of Vibrio bivalvicida Associated With a Massive Larval Mortality Event in a Commercial Hatchery of Scallop Argopecten purpuratus in Chile

Adult spawning and early larval development of the endangered bivalve Pinna nobilis

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