Genome Sequence of Lactococcus lactis subsp.
            <i>lactis</i>
            NCDO 2118, a GABA-Producing Strain

Oliveira, Lívia Costa de; Saraiva, Tessália Diniz Luerce; Soares, Siomar C.; Ramos, Rommel Thiago Jucá; Sá, Pablo Henrique Caracciolo Gomes de; Carneiro, Adriana Ribeiro; Miranda, Fábio; Freire, Matheus; Renan, Wendel; Júnior, Alberto F. O.; Santos, Anderson; Pinto, Anne C.; Souza, Bruna; Castro, Camila Prósperi de; Diniz, Carlos; Rocha, Clarissa Santos; Mariano, Diego; Aguiar, Edgar Lacerda de; Folador, Edson Luiz; Barbosa, Eudes Guilherme Vieria; Aburjaile, Flávia Figueira; Gonçalves, Lucas Amorim; Guimarães, Luís Carlos; Azevedo, Marcela Santiago Pacheco de; Agresti, Pamela C. Mancha; Silva, Renata F.; Tiwari, Sandeep; Almeida, Síntia; Hassan, Syed Shah; Pereira, Vanessa Bastos; Abreu, Vinícius Augusto Carvalho de; Pereira, Ulisses de Pádua; Dorella, Fernanda Alves; Carvalho, Alex F.; Pereira, Felipe Luiz; Leal, Carlos Augusto Gomes; Figueiredo, Henrique César Pereira; Silva, Artur M. S.; Miyoshi, Anderson; Azevedo, Vasco

doi:10.1128/genomea.00980-14

Cited by 25 publications

(16 citation statements)

References 15 publications

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“…The same NRPS/PKS system is also present in the published genomes of L. lactis strains NCDO 2118 (completed, isolated from frozen peas) (Oliveira et al., ) and YF11 (draft, isolated from fermented corn) (Cavanagh, Fitzgerald, & McAuliffe, ; Du et al., ). Genes coding for the NRPS/PKS system were also detected in L. lactis Li‐1, KF134, KF146, and KF196 according to comparative genome hybridization (Siezen et al., ) and confirmed by BLASTp analysis (data not shown).…”

Section: Resultsmentioning

confidence: 91%

The Lactococcus lactis KF147 nonribosomal peptide synthetase/polyketide synthase system confers resistance to oxidative stress during growth on plant leaf tissue lysate

Golomb

Coates

et al. 2017

MicrobiologyOpen

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Strains of Lactococcus lactis isolated from plant tissues possess adaptations that support their survival and growth in plant‐associated microbial habitats. We previously demonstrated that genes coding for a hybrid nonribosomal peptide synthetase/polyketide synthase (NRPS/PKS) system involved in production of an uncharacterized secondary metabolite are specifically induced in L. lactis KF147 during growth on plant tissues. Notably, this NRPS/PKS has only been identified in plant‐isolated strains of L. lactis. Here, we show that the L. lactis KF147 NRPS/PKS genes have homologs in certain Streptococcus mutans isolates and the genetic organization of the NRPS/PKS locus is conserved among L. lactis strains. Using an L. lactis KF147 mutant deficient in synthesis of NrpC, a 4′‐phosphopantetheinyl transferase, we found that the NRPS/PKS system improves L. lactis during growth under oxidative conditions in Arapidopsis thaliana leaf lysate. The NRPS/PKS system also improves tolerance of L. lactis to reactive oxygen species and specifically H2O2 and superoxide radicals in culture medium. These findings indicate that this secondary metabolite provides a novel mechanism for reactive oxygen species detoxification not previously known for this species.

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Section: Resultsmentioning

confidence: 91%

The Lactococcus lactis KF147 nonribosomal peptide synthetase/polyketide synthase system confers resistance to oxidative stress during growth on plant leaf tissue lysate

Golomb

Coates

et al. 2017

MicrobiologyOpen

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“…The availability of whole genome sequences of different strains of L. lactis has enabled the knowledge about molecular bases of the genetic and biochemistry this bacteria (Oliveira et al, 2014;Tschoeke et al, 2014;Zhao et al, 2015). In addition, these genomic studies contributed also to the development of metabolic model for explore the biotechnological potential of L. lactis, through of biology system approach (Flahaut et al, 2013;Dolatshahi et al, 2016).…”

Section: Introductionmentioning

confidence: 99%

“…lactis IL1403 a prototypical strain isolated from a cheese starter culture (Bolotin et al, 2001), L. lactis subsp. lactis NCDO2118 isolated from frozen peas with probiotic characteristic (Luerce et al, 2014;Oliveira et al, 2014), L. lactis subsp. cremoris MG1363 plasmid-free and prophage-cured derivative of NCDO712, extremely used for physiological studies (Gasson, 1983) and L. lactis subsp.…”

Section: Introductionmentioning

confidence: 99%

Comparative proteomic analysis of four biotechnological strains Lactococcus lactis through label‐free quantitative proteomics

Silva¹,

Sousa²,

Oliveira

et al. 2018

Microbial Biotechnology

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Summary Lactococcus lactis is a bacteria with high biotechnological potential, where is frequently used in the amino acid production and production of fermented dairy products, as well as drug delivery systems and mucosal vaccine vector. The knowledge of a functional core proteome is important extremely for both fundamental understanding of cell functions and for synthetic biology applications. In this study, we characterized the L. lacits proteome from proteomic analysis of four biotechnological strains L. lactis : L. lactis subsp. lactis NCDO 2118, L. lactis subsp. lactis IL 1403, L. lactis subsp. cremoris NZ 9000 and L. lactis subsp. cremoris MG 1363. Our label‐free quantitative proteomic analysis of the whole bacterial lysates from each strains resulted in the characterization of the L. lactis core proteome that was composed by 586 proteins, which might contribute to resistance of this bacterium to different stress conditions as well as involved in the probiotic characteristic of L. lactis . Kegg enrichment analysis shows that ribosome, metabolic pathways, pyruvate metabolism and microbial metabolism in diverse environments were the most enriched. According to our quantitative proteomic analysis, proteins related to translation process were the more abundant in the core proteome, which represent an important step in the synthetic biology. In addition, we identified a subset of conserved proteins that are exclusive of the L. lactis subsp. cremoris or L. lactis subsp. lactis , which some are related to metabolic pathway exclusive. Regarding specific proteome of NCDO 2118, we detected ‘strain‐specific proteins’. Finally, proteogenomics analysis allows the identification of proteins, which were not previously annotated in IL 1403 and MG 1363. The results obtained in this study allowed to increase our knowledge about the biology of L. lactis , which contributes to the implementation of strategies that make it possible to increase the biotechnological potential of this bacterium.

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“…lactis NCDO 2118 [24] and 15 other strains of Lactococcus were retrieved from the GENBANK dataset of NCBI (Table 1). Briefly, the dataset is composed of 8 strains of Lactococcus lactis subsp.…”

Section: Methodsmentioning

confidence: 99%

Analyses of the probiotic property and stress resistance-related genes of Lactococcus lactis subsp. lactis NCDO 2118 through comparative genomics and in vitro assays

et al. 2017

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Lactococcus lactis subsp. lactis NCDO 2118 was recently reported to alleviate colitis symptoms via its anti-inflammatory and immunomodulatory activities, which are exerted by exported proteins that are not produced by L. lactis subsp. lactis IL1403. Here, we used in vitro and in silico approaches to characterize the genomic structure, the safety aspects, and the immunomodulatory activity of this strain. Through comparative genomics, we identified genomic islands, phage regions, bile salt and acid stress resistance genes, bacteriocins, adhesion-related and antibiotic resistance genes, and genes encoding proteins that are putatively secreted, expressed in vitro and absent from IL1403. The high degree of similarity between all Lactococcus suggests that the Symbiotic Islands commonly shared by both NCDO 2118 and KF147 may be responsible for their close relationship and their adaptation to plants. The predicted bacteriocins may play an important role against the invasion of competing strains. The genes related to the acid and bile salt stresses may play important roles in gastrointestinal tract survival, whereas the adhesion proteins are important for persistence in the gut, culminating in the competitive exclusion of other bacteria. Finally, the five secreted and expressed proteins may be important targets for studies of new anti-inflammatory and immunomodulatory proteins. Altogether, the analyses performed here highlight the potential use of this strain as a target for the future development of probiotic foods.

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Genome Sequence of Lactococcus lactis subsp. lactis NCDO 2118, a GABA-Producing Strain

Abstract: Lactococcus lactis subsp. lactis NCDO 2118 is a nondairy lactic acid bacterium, a xylose fermenter, and a gamma-aminobutyric acid (GABA) producer isolated from frozen peas. Here, we report the complete genome sequence of L. lactis NCDO 2118, a strain with probiotic potential activity.

Cited by 25 publications

References 15 publications

The Lactococcus lactis KF147 nonribosomal peptide synthetase/polyketide synthase system confers resistance to oxidative stress during growth on plant leaf tissue lysate

The Lactococcus lactis KF147 nonribosomal peptide synthetase/polyketide synthase system confers resistance to oxidative stress during growth on plant leaf tissue lysate

Comparative proteomic analysis of four biotechnological strains Lactococcus lactis through label‐free quantitative proteomics

Analyses of the probiotic property and stress resistance-related genes of Lactococcus lactis subsp. lactis NCDO 2118 through comparative genomics and in vitro assays

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