Genome Sequence of Naphthalene-Degrading Soil Bacterium Pseudomonas putida CSV86

Abstract

Pseudomonas putida CSV86, a soil isolate, preferentially utilizes naphthalene over glucose as a source of carbon and energy. We present the draft genome sequence, which is 6.4 Mb in size; analysis suggests the chromosomal localization of genes coding for naphthalene utilization. The operons coding for glucose and other aromatic compounds might also be annotated in another study.

High Resolution Structures of Periplasmic Glucose-binding Protein of Pseudomonas putida CSV86 Reveal Structural Basis of Its Substrate Specificity

“…The repression of the glucose transport system in the presence of aromatics and organic acids was found to be responsible for this novel property (16,17). The draft genome sequence of this organism has been determined (18,19). It is proposed that the components of glucose transport include an outer membrane protein, OprB (17); a periplasmic glucose-binding protein, GBP (20,21); and a putative glucose ABC transporter (64).…”

Periplasmic glucose‐binding protein from Pseudomonas putida CSV86 – identification of the glucose‐binding pocket by homology‐model‐guided site‐specific mutagenesis

“…The draft genome sequence of this strain has recently been determined [9]. The unique property of CSV86 is its ability to utilize aromatic compounds preferentially to glucose and co-metabolism of aromatic compounds and organic acids [10].…”

“…Aromatic and organic acidmediated repression of OprB (an outer membrane pro-tein that allows the passage of sugar molecules across the outer membrane) [11], periplasmic glucose-binding protein (ppGBP) [12] and the glucose-metabolizing enzyme Zwf [13] was found to be responsible for this ability. In addition, identification of genes encoding various components of the glucose uptake system (which includes OprB, GBP and three subunits of the ATP binding cassette (ABC) transporter) [9] led us to hypothesize the presence of a high-affinity GBP-dependent ABC transporter in P. putida CSV86. Both GBP and OprB were found to be induced by glucose and suppressed by aromatic compounds and organic acids.…”

Isolation and characterization of naphthalene biodegrading Methylobacterium radiotolerans bacterium from the eastern coastline of the Kingdom of Saudi Arabia

“…For instance, P. aeruginosa can grow in the presence of a variety of aliphatic and aromatic compounds such as lactate (Gao et aromatic fl uoranthene, phenanthrene (Zhang et al 2011), hexadecane, benzene, toluene (S Mukherjee et al 2010), paracetamol (Hu et al 2013), and 4-chlorobenzoate (Hoskeri et al 2011). Similar wide range of assimilation of substrates has been reported with other species of the same genus, such as Pseudomanas putida with biodegradation of aromatic compounds (Diaz et al 2008, Ebrahimi and Plettner 2013, El-Naas et al 2009, Fernandez et al 2012, Hwang et al 2009, Li et al 2011, Q Lin and Jianlong 2010, Phale et al 2013, Takeo et al 2006, You et al 2013) and alkane derivatives (Dunn et al 2005, Johnson and Hyman 2006, Smith and Hyman 2004. In line with previous reports, Pseudomonas aeruginosa strain N7B1 had a wide range of substrate specifi city, thus is an important bacterium that could be used in bioremediation strategies.…”

“…This growth was limited with the M. radiotolerans strain but was very high with P. aeruginosa (Table 1), thus the latter strain can effi ciently express catabolic enzymes of the "salicylate pathway". The existence of this pathway has already been proven in P. aeruginosa (Civilini et al 1999, Takizawa et al 1999, and in other bacteria of the genus Pseudomonas (Izmalkova et al 2013, Phale et al 2013. The growth of M. radiotolerans in the presence of salicylic acid or catechol is lower than in the presence of naphthalene, yet one would have expected these single ring molecules to be easily catabolised, thus supporting a higher bacteria growth.…”