Genome Sequence of Rapid Beer-Spoiling Isolate Lactobacillus brevis BSO 464

Bergsveinson, Jordyn; Pittet, Vanessa; Ewen, Emily; Baecker, Nina; Ziola, Barry

doi:10.1128/genomea.01411-15

Cited by 10 publications

(6 citation statements)

References 12 publications

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“…damnosus to the plasmids of the rapid beer spoiling strain L . brevis BSO 464 [ 35 ]. The amount of shared genes is highest for both beer spoiling P .…”

Section: Resultsmentioning

confidence: 99%

“…In case of the plasmid comparison, we included the plasmidome of the rapid beer spoiling L . brevis BSO 464 [ 35 ]. Brewery strains and the winery isolate TMW 2.1536-NB are color labeled for differentiation.…”

Section: Resultsmentioning

confidence: 99%

“…damnosus genomes and the L . brevis BSO 464 genome (BioProject PRJNA203088) [ 35 ]. In addition BADGE was used with two other data sets.…”

Section: Methodsmentioning

confidence: 99%

See 2 more Smart Citations

The Identification of Novel Diagnostic Marker Genes for the Detection of Beer Spoiling Pediococcus damnosus Strains Using the BlAst Diagnostic Gene findEr

et al. 2016

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As the number of bacterial genomes increases dramatically, the demand for easy to use tools with transparent functionality and comprehensible output for applied comparative genomics grows as well. We present BlAst Diagnostic Gene findEr (BADGE), a tool for the rapid prediction of diagnostic marker genes (DMGs) for the differentiation of bacterial groups (e.g. pathogenic / nonpathogenic). DMG identification settings can be modified easily and installing and running BADGE does not require specific bioinformatics skills. During the BADGE run the user is informed step by step about the DMG finding process, thus making it easy to evaluate the impact of chosen settings and options. On the basis of an example with relevance for beer brewing, being one of the oldest biotechnological processes known, we show a straightforward procedure, from phenotyping, genome sequencing, assembly and annotation, up to a discriminant marker gene PCR assay, making comparative genomics a means to an end. The value and the functionality of BADGE were thoroughly examined, resulting in the successful identification and validation of an outstanding novel DMG (fabZ) for the discrimination of harmless and harmful contaminations of Pediococcus damnosus, which can be applied for spoilage risk determination in breweries. Concomitantly, we present and compare five complete P. damnosus genomes sequenced in this study, finding that the ability to produce the unwanted, spoilage associated off-flavor diacetyl is a plasmid encoded trait in this important beer spoiling species.

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“…damnosus to the plasmids of the rapid beer spoiling strain L . brevis BSO 464 [ 35 ]. The amount of shared genes is highest for both beer spoiling P .…”

Section: Resultsmentioning

confidence: 99%

Section: Resultsmentioning

confidence: 99%

See 1 more Smart Citation

The Identification of Novel Diagnostic Marker Genes for the Detection of Beer Spoiling Pediococcus damnosus Strains Using the BlAst Diagnostic Gene findEr

et al. 2016

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“…Recently, L. brevis strain was also reported for its oral probiotic properties [22]. Contrariwise, L. brevis strains have also been reported for the spoilage of beer [23]. L. plantarum is a versatile and heterogeneous bacterium found in fermented foods and feed products, as well as in the human gastrointestinal tract (GIT) [24].…”

Section: Introductionmentioning

confidence: 99%

Tracking of Intentionally Inoculated Lactic Acid Bacteria Strains in Yogurt and Probiotic Powder

2019

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The present work aimed at tracking intentionally inoculated lactic acid bacteria (LAB) strains in yogurt and probiotic powder. Leuconostoc (Leu.) mesenteroides (11251), Lactobacillus (L.) brevis (B151), and Lactobacillus plantarum (LB41K) strains were tracked in yogurt, and L. plantarum (LB41P) was tracked in a commercial probiotic powder. The yogurt was intentionally inoculated with the selected bacterial strains. Two types of yogurt with known and unknown bacterial pools were utilized. The standard 16S rRNA gene sequencing was used to evaluate the initial screening. The molecular typing tools, random amplified polymorphic DNA (RAPD), repetitive element palindromic PCR (rep-PCR), and comparative gene sequence analysis of selected housekeeping loci were used to track the inoculated dubious strains. Out of 30 random selections for each inoculation, the developed method identified seven (11251), nine (B151), and five (LB41K) colonies in the yogurt. The validation was performed by identifying 7 colonies (LB41P) out of 30 in the probiotic powder. The DNA banding profiles and the gene sequence alignments led to the identification of the correct inoculated strains. Overall, the study summarizes the use of molecular tools to identify the deliberately inoculated LAB strains. In conclusion, the proposed polyphasic approach effectively tracked the intentionally inoculated strains: Leu. mesenteroides, L. brevis, and L. plantarum (LB41K) in yogurt and L. plantarum (LB41P) in probiotic powder. The study demonstrates how to track industrially relevant misused LAB strains in marketable food products.

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“…Conversely, Lb. brevis strains are also associated with food and beverage spoilage, particularly that of beer [4,5]. Beer is generally regarded as a harsh environment for microorganisms [5,6].…”

Section: Introductionmentioning

confidence: 99%

Isolation and Characterization of Lactobacillus brevis Phages

Feyereisen

Mahony

Lugli

et al. 2019

Viruses

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Lactobacillus brevis has been widely used in industry for fermentation purposes. However, it is also associated with the spoilage of foods and beverages, in particular, beer. There is an increasing demand for natural food preservation methods, and in this context, bacteriophages possess the potential to control such spoilage bacteria. Just a few studies on phages infecting Lactobacillus brevis have been performed to date and in the present study, we report the isolation and characterization of five virulent phages capable of infecting Lb. brevis strains. The analysis reveals a high diversity among the isolates, with members belonging to both, the Myoviridae and Siphoviridae families. One isolate, designated phage 3-521, possesses a genome of 140.8 kb, thus representing the largest Lb. brevis phage genome sequenced to date. While the isolated phages do not propagate on Lb. brevis beer-spoiling strains, phages showed activity against these strains, impairing the growth of some Lb. brevis strains. The results highlight the potential of bacteriophage-based treatments as an effective approach to prevent bacterial spoilage of beer.

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Genome Sequence of Rapid Beer-Spoiling Isolate Lactobacillus brevis BSO 464

Cited by 10 publications

References 12 publications

The Identification of Novel Diagnostic Marker Genes for the Detection of Beer Spoiling Pediococcus damnosus Strains Using the BlAst Diagnostic Gene findEr

The Identification of Novel Diagnostic Marker Genes for the Detection of Beer Spoiling Pediococcus damnosus Strains Using the BlAst Diagnostic Gene findEr

Tracking of Intentionally Inoculated Lactic Acid Bacteria Strains in Yogurt and Probiotic Powder

Isolation and Characterization of Lactobacillus brevis Phages

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