Genome Sequence of Rhodococcus erythropolis XP, a Biodesulfurizing Bacterium with Industrial Potential

Tao, Fei; Zhao, Peng; Li, Qian; Su, Fei; Yu, Bo; Ma, Cuiqing; Tang, Hongzhi; Tai, Cui; Wu, Geng; Xu, Ping

doi:10.1128/jb.06154-11

Cited by 28 publications

(10 citation statements)

References 14 publications

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“…Importantly, from translational perspective, drugs inhibiting glutamine and glutamate synthesis results in disruption of the biofilm structure and biomass, as well as in interference with the metabolic cooperation between the cells residing in different biofilm layers. These results imply that disturbing glutamine and glutamate metabolism could potentially serve to inhibit biofilm formation, an immense need in different ecological, medical and biotechnological settings 71 – 73 .…”

Section: Discussionmentioning

confidence: 99%

Novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine

Hassanov¹,

Karunker²,

Steinberg³

et al. 2018

Sci Rep

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Bacteria in nature often reside in differentiated communities termed biofilms, which are an active interphase between uni-cellular and multicellular life states for bacteria. Here we demonstrate that the development of B. subtilis biofilms is dependent on the use of glutamine or glutamate as a nitrogen source. We show a differential metabolic requirement within the biofilm; while glutamine is necessary for the dividing cells at the edges, the inner cell mass utilizes lactic acid. Our results indicate that biofilm cells preserve a short-term memory of glutamate metabolism. Finally, we establish that drugs that target glutamine and glutamate utilization restrict biofilm development. Overall, our work reveals a spatial regulation of nitrogen and carbon metabolism within the biofilm, which contributes to the fitness of bacterial complex communities. This acquired metabolic division of labor within biofilm can serve as a target for novel anti-biofilm chemotherapies

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Section: Discussionmentioning

confidence: 99%

Novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine

Hassanov¹,

Karunker²,

Steinberg³

et al. 2018

Sci Rep

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“…S11). Comparatively, the Rhodococcus erythropolis 39 NusA that inherently lacks a C-terminal extension (Fig. S10) showed temperature sensitivity and deficiency in oligomerization (Fig.…”

Section: Resultsmentioning

confidence: 99%

“…The recombinant plasmids were transformed into the E. coli BL21 (DE3) strain. The nusA genes from P. aeruginosa PAO121, P. putida KT244022, L. bulgaricus ATCC 1184223, B. coagulans 2–624 and R. erythropolis XP39 as well as nusA(1–343) from strain ATCC 11842 were also cloned and ligated to the pET-28a vector for expression.…”

Section: Methodsmentioning

confidence: 99%

Escherichia coli transcription termination factor NusA: heat-induced oligomerization and chaperone activity

Jiang

et al. 2013

Sci Rep

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Escherichia coli NusA, an essential component of the RNA polymerase elongation complex, is involved in transcriptional elongation, termination, anti-termination, cold shock and stress-induced mutagenesis. In this study, we demonstrated that NusA can self-assemble into oligomers under heat shock conditions and that this property is largely determined by the C-terminal domain. In parallel with the self-assembly process, NusA also acquires chaperone activity. Furthermore, NusA overexpression results in the enhanced heat shock resistance of host cells, which may be due to the chaperone activity of NusA. Our results suggest that E. coli NusA can act as a protector to prevent protein aggregation under heat stress conditions in vitro and in the NusA-overexpressing strain. We propose a new hypothesis that NusA could serve as a molecular chaperone in addition to its functions as a transcription factor. However, it remains to be further investigated whether NusA has the same function under normal physiological conditions.

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“…Mycobacterium tuberculosis NCGM2209, sequence accession DF126614 and DF126615 [52] Rhodococcus erythropolis strain XP, sequence accession AGCF01000000 [53] Serinicoccus profundi MCCC 1A05965 T , sequence accession AFYF00000000 [54] Phylum Spirochaetes Leptospira interrogans, sequence accession CP001221 (CI), CP001222 (CII) [55] Phylum Bacteroidetes…”

Section: Standards In Genomic Sciencesmentioning

confidence: 99%

Genome sequences published outside of Standards in Genomic Sciences, October – November 2011

Nelson

2011

Stand. Genomic Sci.

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Genome Sequence of Rhodococcus erythropolis XP, a Biodesulfurizing Bacterium with Industrial Potential

Cited by 28 publications

References 14 publications

Novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine

Novel antibiofilm chemotherapies target nitrogen from glutamate and glutamine

Escherichia coli transcription termination factor NusA: heat-induced oligomerization and chaperone activity

Genome sequences published outside of Standards in Genomic Sciences, October – November 2011

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