Genome Sequence of the Alphaproteobacterium Blastochloris sulfoviridis DSM 729, Which Requires Reduced Sulfur as a Growth Supplement and Contains Bacteriochlorophyll
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Kyndt, John A.; Salama, Dayana Montano; Meyer, Terry E.

doi:10.1128/mra.00313-20

Cited by 7 publications

(4 citation statements)

References 16 publications

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“…Interestingly, this genus is able to fix nitrogen via an oxygen‐sensitive nitrogenase (Dedysh et al ., 2000; Dunfield et al ., 2003; Dedysh et al ., 2004). In addition, Methylovirgula ligni , also acidiphilic, contains fragments of the nifH gene and is capable of nitrogen fixation (Vorob'ev et al ., 2009) and Blastochloris species have two sets of enzymes (Nif and Anf) for nitrogen fixation (Kyndt et al ., 2020) (Table 1). We did not find information concerning the nitrogen fixing capacity of Rhodoplanes sp.…”

Section: Discussionmentioning

confidence: 99%

Into the wild blueberry (Vaccinium angustifolium) rhizosphere microbiota

Morvan

Meglouli

Sahraoui

et al. 2020

Environmental Microbiology

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The ability of wild blueberries to adapt to their harsh environment is believed to be closely related to their symbiosis with ericoid mycorrhizal fungi, which produce enzymes capable of organic matter mineralization. Although some of these fungi have been identified and characterized, we still know little about the microbial ecology of wild blueberry. Our study aims to characterize the fungal and bacterial rhizosphere communities of Vaccinium angustifolium (the main species encountered in wild blueberry fields). Our results clearly show that the fungal order Helotiales was the most abundant taxon associated with V. angustifolium. Helotiales contains most of the known ericoid mycorrhizal fungi which are expected to dominate in such a biotope. Furthermore, we found the dominant bacterial order was the nitrogenfixing Rhizobiales. The Bradyrhizobium genus, whose members are known to form nodules with legumes, was among the 10 most abundant genera in the bacterial communities. In addition, Bradyrhizobium and Roseiarcus sequences significantly correlated with higher leaf-nitrogen content. Overall, our data documented fungal and bacterial community structure differences in three wild blueberry production fields.

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Section: Discussionmentioning

confidence: 99%

Into the wild blueberry (Vaccinium angustifolium) rhizosphere microbiota

Morvan

Meglouli

Sahraoui

et al. 2020

Environmental Microbiology

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“…In each case, the common components of the photosystem are encoded by single ORFs within the photosynthesis gene cluster (PGC), which also contains genes encoding pigment biosynthesis enzymes and photosystem assembly factors [45]. However, each sequenced strain contains multiple LH1γ genes that are located distant from the PGC [24,25,46,47]. This suggests that these genes evolved separately from the other PGC genes, and that assembly of RC-LH1 without LH1γ is safeguarded against by the presence of multiple paralogous genes in the genome.…”

Section: Discussionmentioning

confidence: 99%

The role of the γ subunit in the photosystem of the lowest-energy phototrophs

Namoon

Rudling

Canniffe

2022

Preprint

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Purple phototrophic bacteria use a core "photosystem" consisting of light harvesting antenna complex 1 (LH1) surrounding the reaction centre (RC), which primarily absorbs far-red/near-infrared light and converts it to chemical energy. Species in the Blastochloris genus, which are able to use light >1000nm for photosynthesis, use bacteriochlorophyll (BChl) b rather than the more common BChl a as their major photopigment, and also uniquely assemble LH1 with an additional polypeptide subunit, LH1γ, encoded by multiple open reading frames in their genomes. In order to assign a role to this subunit, we deleted the four LH1γ-encoding genes in the model Blastochloris viridis. Interestingly, growth under halogen bulbs routinely used for cultivation of anoxygenic phototrophs yielded cells displaying an absorption maximum of 825 nm, similar to that of the RC complex without LH1, but growth under white light from fluorescent bulbs yielded cells with an absorption maximum at 972 nm. HPLC analysis of pigment composition and sucrose density gradient fractionation demonstrate that the mutant grown in white light assembles RC-LH1, albeit with an absorption maximum blue-shifted by 46 nm relative to the WT complex. Wavelengths between 900-1000 nm transmit poorly through the atmosphere due to strong absorption by water, thus our results provide an evolutionary rationale for the incorporation of the γ subunit into the LH1 ring; this polypeptide red-shifts the absorption maximum of the complex to a range of the spectrum where the photons are of lower energy but are more abundant. Finally, we transformed the mutant with plasmids carrying genes encoding natural LH1γ variants and demonstrate that the polypeptide found in the WT complex red-shifts absorption back to 1018 nm, but incorporation of a distantly-related variant results in only a moderate red-shift. This result suggests that tuning the absorption maximum of this organism is possible, and may permit light capture past the current low-energy limit of natural photosynthesis.

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“…The Blastochloris genus had placements in a long branch starting deep in the phylogenetic tree, but not in the tips. The separation of Blastochloris from other Rhizobacteria has been explained [13], since this genus uses bacteriochlorophyll b instead of bacteriocholophyll a in its reaction center [22]. The placement of metagenomic reads at the basal branch of this group suggests that other lineages, related to the Blastochloris references, but with different PufLM genes, exist in the bark communities of avocado.…”

Section: Avocado Bark Rhizobialesmentioning

confidence: 99%

“…The presence of Blastochloris reads in bark microbial communities, different from the reference sequences, is interesting, as only four species from this genus are known [22]. Mapping of reads from the avocado bark metagenome to Methylocella species PufLM suggests the posibility of methanotrophy in this environment.…”

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confidence: 94%

Type II Photosynthetic Reaction Center Genes of Avocado (Persea americana Mill.) Bark Microbial Communities are Dominated by Aerobic Anoxygenic Alphaproteobacteria

Aguirre‐von‐Wobeser

2021

Curr Microbiol

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The tree bark environment is an important microbial habitat distributed worldwide on thrillions of trees. However, the microbial communities of tree bark are largely unknown, with most studies on plant aerial surfaces focused on the leaves. Recently, we presented a metagenomic study of bark microbial communities from avocado. In these communities, oxygenic and anoxygenic photosynthesis genes were very abundant, especially when compared to rhizospheric soil from the same trees. In this work, Evolutionary Placement Algorithm analysis was performed on metagenomic reads orthologous to the PufLM gene cluster, encoding for the bacterial type II photosynthetic reaction center. These photosynthetic genes were found affiliated to different groups of bacteria, mostly aerobic anoxygenic photosynthetic Alphaproteobacteria, including Sphingomonas, Methylobacterium and several Rhodospirillales. These results suggest that anoxygenic photosynthesis in avocado bark microbial communities functions primarily as additional energy source for heterotrophic growth. Together with our previous results, showing a large abundance of cyanobacteria in these communities, a picture emerges of the tree holobiont, where light penetrating the trees canopies and reaching the inner stems, including the trunk, is probably utilized by cyanobacteria for oxygenic photosynthesis, and the far-red light aids the growth of aerobic anoxygenic photosynthetic bacteria.

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Genome Sequence of the Alphaproteobacterium Blastochloris sulfoviridis DSM 729, Which Requires Reduced Sulfur as a Growth Supplement and Contains Bacteriochlorophyll b

Cited by 7 publications

References 16 publications

Into the wild blueberry (Vaccinium angustifolium) rhizosphere microbiota

Into the wild blueberry (Vaccinium angustifolium) rhizosphere microbiota

The role of the γ subunit in the photosystem of the lowest-energy phototrophs

Type II Photosynthetic Reaction Center Genes of Avocado (Persea americana Mill.) Bark Microbial Communities are Dominated by Aerobic Anoxygenic Alphaproteobacteria

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