Genome Sequence of the Cellulosome-Producing Mesophilic Organism
            <i>Clostridium cellulovorans</i>
            743B

Tamaru, Yutaka; Miyake, Hideo; Kuroda, Kouichi; Nakanishi, Akihito; Kawade, Yujiro; Yamamoto, Kousuke; Uemura, Masaaki; Fujita, Yasuhiro; Doi, Roy H.; Ueda, Mitsuyoshi

doi:10.1128/jb.01450-09

Cited by 67 publications

(53 citation statements)

References 10 publications

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“…To understand the molecular mechanism underlying saccharification by the cellulosome, genomic analysis of several cellulosome-producing microorganisms was performed (Clostridium thermocellum and Clostridium cellulolyticum, analyzed by the Joint Genome Institute in 2007 and 2009, respectively). Whole-genome sequence analysis of Clostridium cellulovorans, which was suggested to have protoplast formation activity (1,16), identified putative cellulosomal genes (14). Compared to genomes of other cellulosomal clostridia, C. cellulovorans has the smallest number of cellulosomal genes (15).…”

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confidence: 99%

Putative Role of Cellulosomal Protease Inhibitors in Clostridium cellulovorans Based on Gene Expression and Measurement of Activities

et al. 2011

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This study is the first to demonstrate the activity of putative cellulosomal protease/peptidase inhibitors (named cyspins) of Clostridium cellulovorans, using the Saccharomyces cerevisiae display system. Cyspins exhibited inhibitory activities against several representative plant proteases. This suggests that these inhibitors protect their microbe and cellulosome from external attack by plant proteases.

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Putative Role of Cellulosomal Protease Inhibitors in Clostridium cellulovorans Based on Gene Expression and Measurement of Activities

et al. 2011

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“…However, the molecular mechanism of the formation of the cellulosome has not been characterized (7). Previously, we sequenced the entire genome of Clostridium cellulovorans and identified all genes, including those that encode proteins of known and unknown functions, related to cellulosomal composition (8). Genome analysis of C. cellulovorans indicated the presence of 57 cellulosomal proteinencoding genes and 168 noncellulosomal (hemi)cellulolytic protein-encoding genes.…”

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Exoproteome Profiles of Clostridium cellulovorans Grown on Various Carbon Sources

Matsui

Bae

Esaka

et al. 2013

Appl Environ Microbiol

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The cellulosome is a complex of cellulosomal proteins bound to scaffolding proteins. This complex is considered the most efficient system for cellulose degradation. Clostridium cellulovorans, which is known to produce cellulosomes, changes the composition of its cellulosomes depending on the growth substrates. However, studies have investigated only cellulosomal proteins; profile changes in noncellulosomal proteins have rarely been examined. In this study, we performed a quantitative proteome analysis of the whole exoproteome of C. cellulovorans, including cellulosomal and noncellulosomal proteins, to illustrate how various substrates are efficiently degraded. C. cellulovorans was cultured with cellobiose, xylan, pectin, or phosphoric acid-swollen cellulose (PASC) as the sole carbon source. PASC was used as a cellulose substrate for more accurate quantitative analysis. Using an isobaric tag method and a liquid chromatography mass spectrometer equipped with a long monolithic silica capillary column, 639 proteins were identified and quantified in all 4 samples. Among these, 79 proteins were involved in saccharification, including 35 cellulosomal and 44 noncellulosomal proteins. We compared protein abundance by spectral count and found that cellulosomal proteins were more abundant than noncellulosomal proteins. Next, we focused on the fold change of the proteins depending on the growth substrates. Drastic changes were observed mainly among the noncellulosomal proteins. These results indicate that cellulosomal proteins were primarily produced to efficiently degrade any substrate and that noncellulosomal proteins were specifically produced to optimize the degradation of a particular substrate. This study highlights the importance of noncellulosomal proteins as well as cellulosomes for the efficient degradation of various substrates.

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“…Finally, genes encoding structural components of cellulosomes, such as dockerins, were found throughout the genome. The genome of R. albus 7 will be useful for comparative analyses with other sequenced Ruminococcus (2) and Clostridium (12,14) genomes, and, given that ethanol is its major fermentation product, will serve as a useful model for biofuel production.…”

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Complete Genome of the Cellulolytic Ruminal Bacterium Ruminococcus albus 7

et al. 2011

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Genome Sequence of the Cellulosome-Producing Mesophilic Organism Clostridium cellulovorans 743B

Cited by 67 publications

References 10 publications

Putative Role of Cellulosomal Protease Inhibitors in Clostridium cellulovorans Based on Gene Expression and Measurement of Activities

Putative Role of Cellulosomal Protease Inhibitors in Clostridium cellulovorans Based on Gene Expression and Measurement of Activities

Exoproteome Profiles of Clostridium cellulovorans Grown on Various Carbon Sources

Complete Genome of the Cellulolytic Ruminal Bacterium Ruminococcus albus 7

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