Genome sequence of the copper resistant and acid-tolerant Desulfosporosinus sp. BG isolated from the tailings of a molybdenum-tungsten mine in the Transbaikal area

Карначук, О. В.; Kadnikov, Vitalii V.; Panova, I. A.; Mardanov, Andrey V.; Beletsky, Alexey V.; Danilova, Erzhena V.; Avakyan, Marat R.; Ravin, Nikolai V.

doi:10.1016/j.gdata.2016.12.014

Cited by 8 publications

(4 citation statements)

References 23 publications

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“…Desulfosporosinus (the family Peptococcaceae ) representatives are known to populate acidic settings, those include few acidophilic sulfate-reducers isolated so far in pure cultures: D. acidiphilus from a mining site at Chessy-Les-Mines, France (Alazard et al, 2010), D. acididurans from White River sediment in Montserrat (Sánchez-Andrea et al, 2015) and Desulfosporosinus sp. strain BG from oxidized mining wastes in Northern Transbaikalia (Karnachuk et al, 2015, 2016). The latter was reported to tolerate high concentrations of copper and produce crystalline copper sulfides (Karnachuk et al, 2015).…”

Section: Resultsmentioning

confidence: 99%

“…Numerous natural and mining-impacted acidic sites of Eastern Transbaikalia, Russia, are located in the area of headwaters of the river Amur, more than 1,000 km eastwards of the lake Baikal, and represent a generous source of distinctive extreme environments. This area remains microbiologically under sampled with only a small number of recent studies available that were mostly dedicated to the diversity of sulfate-reducing bacteria (Karnachuk et al, 2015, 2016; Kadnikov et al, 2016 and others). The present work is focused on the elucidation of diversity of microbial inhabitants in three different types of acidic settings in this area.…”

Section: Introductionmentioning

confidence: 99%

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Microbial Communities of Polymetallic Deposits’ Acidic Ecosystems of Continental Climatic Zone With High Temperature Contrasts

Гаврилов¹,

Korzhenkov

Kublanov³

et al. 2019

Front. Microbiol.

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Acid mine drainage (AMD) systems are globally widespread and are an important source of metal pollution in riverine and coastal systems. Microbial AMD communities have been extensively studied for their ability to thrive under extremely acidic conditions and for their immense contribution to the dissolution of metal ores. However, little is known on microbial inhabitants of AMD systems subjected to extremely contrasting continental seasonal temperature patterns as opposed to maritime climate zones, experiencing much weaker annual temperature variations. Here, we investigated three types of AMD sites in Eastern Transbaikalia (Russia). In this region, all surface water bodies undergo a deep and long (up to 6 months) freezing, with seasonal temperatures varying between −33 and +24°C, which starkly contrasts the common well-studied AMD environments. We sampled acidic pit lake (Sherlovaya Gora site) located in the area of a polymetallic deposit, acidic drainage water from Bugdaya gold-molybdenum-tungsten deposit and Ulan-Bulak natural acidic spring. These systems showed the abundance of bacteria-derived reads mostly affiliated with Actinobacteria, Acidobacteria, Alpha- and Gammaproteobacteria , chloroplasts, Chloroflexi, Bacteroidetes , and Firmicutes . Furthermore, candidate taxa “ Ca . Saccharibacteria” (previously known as TM7), “ Ca . Parcubacteria” (OD1) and WPS-2 were represented in substantial quantities (10–20%). Heterotrophy and iron redox cycling can be considered as central processes of carbon and energy flow for majority of detected bacterial taxa. Archaea were detected in low numbers, with Terrestrial Miscellaneous Euryarchaeal Group (TMEG), to be most abundant (3%) in acidic spring Ulan-Bulak. Composition of these communities was found to be typical in comparison to other AMD sites; however, certain groups (as Ignavibacteriae ) could be specifically associated with this area. This study provides insight into the microbial diversity patterns in acidic ecosystems formed in areas of polymetallic deposits in extreme continental climate zone with contrasting temperature parameters.

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Section: Resultsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

Microbial Communities of Polymetallic Deposits’ Acidic Ecosystems of Continental Climatic Zone With High Temperature Contrasts

Гаврилов¹,

Korzhenkov

Kublanov³

et al. 2019

Front. Microbiol.

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“…An acidophilic Desulfosporosinus sp. BG from a tungsten mine waste in Bom-Gorkhon in Transbaikalia tolerated the presence of copper up to a concentration of 78 mM [5, 14]. Various metal transporters and sequestration by polyphosphates were revealed as possible mechanisms to survive high copper concentrations in Desulfosporosinus sp.…”

Section: Full-textmentioning

confidence: 99%

Desulfosporosinus metallidurans sp. nov., an acidophilic, metal-resistant sulfate-reducing bacterium from acid mine drainage

Panova

Ikkert

Avakyan

et al. 2021

International Journal of Systematic and Evolutionary Microbiology

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A novel, spore-forming, acidophilic and metal-resistant sulfate-reducing bacterium, strain OLT, was isolated from a microbial mat in a tailing dam at a gold ore mining site. Cells were slightly curved immotile rods, 0.5 µm in diameter and 2.0–3.0 µm long. Cells were stained Gram-negative, despite the Gram-positive cell structure revealed by electron microscopy of ultrathin layers. OLT grew at pH 4.0–7.0 with an optimum at 5.5. OLT utilised H2, lactate, pyruvate, malate, formate, propionate, ethanol, glycerol, glucose, fructose, sucrose, peptone and tryptone as electron donors for sulfate reduction. Sulfate, sulfite, thiosulfate, nitrate and fumarate were used as electron acceptors in the presence of lactate. Elemental sulfur, iron (III), and arsenate did not serve as electron acceptors. The major cellular fatty acids were C16:1ω7c (39.0 %) and C16 : 0 (12.1 %). The draft genome of OLT was 5.29 Mb in size and contained 4909 protein-coding genes. The 16S rRNA gene sequence placed OLT within the phylum Firmicutes , class Clostridia , family Peptococcaceae , genus Desulfosporosinus. Desulfosporosinus nitroreducens 59.4BT was the closest relative with 97.6 % sequence similarity. On the basis of phenotypic and phylogenetic characteristics, strain OLT represents a novel species within the genus Desulfosporosinus , for which we propose the name Desulfosporosinus metallidurans sp. nov. with the type strain OLT (=DSM 104464T=VKM В−3021T).

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“…Hitherto, two acidophilic SRB, Desulfosporosinus acidiphilus and Desulfosporosinus acididurans, have been characterised by Alazard et al (2010) and Sánchez-Andrea et al (2015). Several undescribed members of this genus have been reported that grow in acidic media (Abicht et al 2011;Jameson et al 2010;Karnachuk et al 2009Karnachuk et al , 2015aKarnachuk et al , 2017Küsel et al 2001;Senko et al 2009). Another member of the Firmicutes phylum, the thermophilic SRB Thermodesulfobium narugense isolated from a hot spring, has a slightly acidic pH optimum of 5.5-6.0 for growth (Mori et al 2003).…”

Section: Introductionmentioning

confidence: 99%

Selection for novel, acid-tolerant Desulfovibrio spp. from a closed Transbaikal mine site in a temporal pH-gradient bioreactor

Antsiferov

Fyodorova

Kovalyova

et al. 2017

Antonie van Leeuwenhoek

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Almost all the known isolates of acidophilic or acid-tolerant sulphate-reducing bacteria (SRB) belong to the spore-forming genus Desulfosporosinus in the Firmicutes. The objective of this study was to isolate acidophilic/acid-tolerant members of the genus Desulfovibrio belonging to deltaproteobacterial SRB. The sample material originated from microbial mat biomass submerged in mine water and was enriched for sulphate reducers by cultivation in anaerobic medium with lactate as an electron donor. A stirred tank bioreactor with the same medium composition was inoculated with the sulphidogenic enrichment. The bioreactor was operated with a temporal pH gradient, changing daily, from an initial pH of 7.3 to a final pH of 3.7. Among the bacteria in the bioreactor culture, Desulfovibrio was the only SRB group retrieved from the bioreactor consortium as observed by 16S rRNA-targeted denaturing gradient gel electrophoresis. Moderately acidophilic/acid-tolerant isolates belonged to Desulfovibrio aerotolerans-Desulfovibrio carbinophilus-Desulfovibrio magneticus and Desulfovibrio idahonensis-Desulfovibrio mexicanus clades within the genus Desulfovibrio. A moderately acidophilic strain, Desulfovibrio sp. VK (pH optimum 5.7) and acid-tolerant Desulfovibrio sp. ED (pH optimum 6.6) dominated in the bioreactor consortium at different time points and were isolated in pure culture.

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Genome sequence of the copper resistant and acid-tolerant Desulfosporosinus sp. BG isolated from the tailings of a molybdenum-tungsten mine in the Transbaikal area

Cited by 8 publications

References 23 publications

Microbial Communities of Polymetallic Deposits’ Acidic Ecosystems of Continental Climatic Zone With High Temperature Contrasts

Microbial Communities of Polymetallic Deposits’ Acidic Ecosystems of Continental Climatic Zone With High Temperature Contrasts

Desulfosporosinus metallidurans sp. nov., an acidophilic, metal-resistant sulfate-reducing bacterium from acid mine drainage

Selection for novel, acid-tolerant Desulfovibrio spp. from a closed Transbaikal mine site in a temporal pH-gradient bioreactor

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