Genome Sequence of the Corynebacterium pseudotuberculosis Cp316 Strain, Isolated from the Abscess of a Californian Horse

Abstract

The bacterium Corynebacterium pseudotuberculosis is of major veterinary importance because it affects livestock, particularly sheep, goats, and horses, in several countries, including Australia, Brazil, the United States, and Canada, resulting in significant economic losses. In the present study, we describe the complete genome of the Corynebacterium pseudotuberculosis Cp316 strain, biovar equi, isolated from the abscess of a North American horse.

The Pan-Genome of the Animal Pathogen Corynebacterium pseudotuberculosis Reveals Differences in Genome Plasticity between the Biovar ovis and equi Strains

“…Specific patterns of deletions in PiCps 4, 5 and 9 of C. pseudotuberculosis CIP52.97, 316 and 258 (biovar equi strains) have been demonstrated [54]–[56]. Here, we detected the same deletions in all the biovar equi strains, which indicates that these deletion events were specific to the mentioned biovar (Figure S1).…”

“…In previous studies, seven PAIs were identified in C. pseudotuberculosis biovar ovis strains 1002 and C231 (PiCps 1–7) [43], and four additional PAIs have been identified in C. pseudotuberculosis strain 1002 by further comparisons with C. pseudotuberculosis strains 316 and 258 (PiCps 8–11) [54]–[56]. The latter subset of PAIs was identified due to a better view of the two biovars and their specific patterns of plasticity.…”

Identification ofCorynebacterium pseudotuberculosisisolated from muscular abscesses in two horses: First report in Mexico

“…Thus, this PCR product was purified using a QIAquick gel extraction (Altschul et al 1990), and a multiple sequence alignment was carried out in the Vector NTI program 5 and Clone Manager software 8 to compare our sequences (including 2 horses, 2 sheep, and 5 goats isolated from different Mexican states) to the sequences in GenBank with the highest identity percentages. The results showed 100% and 99% (first and second strains, respectively) identify percentages with C. pseudotuberculosis 316 (CP003077.1|) biovar equi isolated from a horse (Ramos et al 2012). The multiple-sequence alignment revealed 4 polymorphic sites between biovar ovis and biovar equi strains (Supplementary Item 1).…”

“…The results showed 100% and 99% (first and second strains, respectively) identify percentages with C. pseudotuberculosis 316 (CP003077.1|) biovar equi isolated from a horse (Ramos et al . ). The multiple‐sequence alignment revealed 4 polymorphic sites between biovar ovis and biovar equi strains ( Supplementary Item 1 ).…”

The Family Corynebacteriaceae

“…The first next-generation DNA sequencing studies have focused on the genomes of corynebacterial pathogens because of their importance in human and animal disease. Complete genome sequences are meanwhile available for a black-pigmented Corynebacterium aurimucosum isolate (Trost et al 2010a), multiple toxigenic and non-toxigenic strains of Corynebacterium diphtheriae (Cerdeño-Tarrága et al 2003;Trost et al 2012), Corynebacterium jeikeium , Corynebacterium kroppenstedtii (Tauch et al 2008a), multiple strains of the Corynebacterium pseudotuberculosis biovars ovis and equi (Trost et al 2010b;Cerdeira et al 2011a, b;Ruiz et al 2011;Silva et al 2011Silva et al , 2012Hassan et al 2012;Lopes et al 2012;Pethick et al 2012a, b;Ramos et al 2012), Corynebacterium resistens (Schröder et al 2012a), Corynebacterium ulcerans from human and animal sources Sekizuka et al 2012), and Corynebacterium urealyticum (Tauch et al 2008b). Complete genome sequences from environmental corynebacteria include Corynebacterium efficiens , Corynebacterium glutamicum (Kalinowski et al 2003;Ikeda and Nakagawa 2003;Yukawa et al 2007), and Corynebacterium variabile .…”