Genome Sequence of the Halophilic Archaeon Halococcus hamelinensis

Burns, Brendan P.; Gudhka, Reema K.; Neilan, Brett A.

doi:10.1128/jb.06599-11

Cited by 15 publications

(9 citation statements)

References 15 publications

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“…The genome sequence of Hcc. hamelinensis 100A6 T has recently been published [ 25 ]. This allows preliminary comparisons with the genome content of other halococci, since as noted above, all halococci appear to be closely related.…”

Section: Resultsmentioning

confidence: 99%

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Properties of Halococcus salifodinae, an Isolate from Permian Rock Salt Deposits, Compared with Halococci from Surface Waters

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Dornmayr-Pfaffenhuemer

et al. 2013

Life

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Halococcus salifodinae BIpT DSM 8989T, an extremely halophilic archaeal isolate from an Austrian salt deposit (Bad Ischl), whose origin was dated to the Permian period, was described in 1994. Subsequently, several strains of the species have been isolated, some from similar but geographically separated salt deposits. Hcc. salifodinae may be regarded as one of the most ancient culturable species which existed already about 250 million years ago. Since its habitat probably did not change during this long period, its properties were presumably not subjected to the needs of mutational adaptation. Hcc. salifodinae and other isolates from ancient deposits would be suitable candidates for testing hypotheses on prokaryotic evolution, such as the molecular clock concept, or the net-like history of genome evolution. A comparison of available taxonomic characteristics from strains of Hcc. salifodinae and other Halococcus species, most of them originating from surface waters, is presented. The cell wall polymer of Hcc. salifodinae was examined and found to be a heteropolysaccharide, similar to that of Hcc. morrhuae. Polyhydroxyalkanoate granules were present in Hcc. salifodinae, suggesting a possible lateral gene transfer before Permian times.

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Section: Resultsmentioning

confidence: 99%

“…Recently, the first genome sequence of a halococcus, Hcc. hamelinensis 100A6 T , became available [ 25 ] and therefore information for several genes ( phaC synthases; subunit A of the rotary A-ATPase) is examined here for their potential use in delineating the evolution of haloarchaeal cocci.…”

Section: Introductionmentioning

confidence: 99%

Properties of Halococcus salifodinae, an Isolate from Permian Rock Salt Deposits, Compared with Halococci from Surface Waters

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Denner

Dornmayr-Pfaffenhuemer

et al. 2013

Life

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“…The archaea in Shark Bay microbial mats are principally halophiles, and many unclassified haloarchaea have been detected from Shark Bay microbial mats and stromatolites and not the surrounding seawater , indicating that these systems are a potential reservoir for these archaea. Halococcus hamelinensis, Haloferax elongans and Haloferax mucosum are haloarchaea that have been isolated from Shark Bay microbialites (Goh et al, 2006;Allen et al, 2008), and several studies have also characterized the genomes of these archaea (Burns et al, 2012;Lynch et al, 2012;Guddhka et al, 2015). Our data indicate evidence for higher abundance of haloarchaea among the contigs in Shark Bay than in Highbourne Cay contigs.…”

Section: Resultsmentioning

confidence: 99%

Unravelling core microbial metabolisms in the hypersaline microbial mats of Shark Bay using high-throughput metagenomics

et al. 2015

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Modern microbial mats are potential analogues of some of Earth's earliest ecosystems. Excellent examples can be found in Shark Bay, Australia, with mats of various morphologies. To further our understanding of the functional genetic potential of these complex microbial ecosystems, we conducted for the first time shotgun metagenomic analyses. We assembled metagenomic nextgeneration sequencing data to classify the taxonomic and metabolic potential across diverse morphologies of marine mats in Shark Bay. The microbial community across taxonomic classifications using protein-coding and small subunit rRNA genes directly extracted from the metagenomes suggests that three phyla Proteobacteria, Cyanobacteria and Bacteriodetes dominate all marine mats. However, the microbial community structure between Shark Bay and Highbourne Cay (Bahamas) marine systems appears to be distinct from each other. The metabolic potential (based on SEED subsystem classifications) of the Shark Bay and Highbourne Cay microbial communities were also distinct. Shark Bay metagenomes have a metabolic pathway profile consisting of both heterotrophic and photosynthetic pathways, whereas Highbourne Cay appears to be dominated almost exclusively by photosynthetic pathways. Alternative non-rubisco-based carbon metabolism including reductive TCA cycle and 3-hydroxypropionate/4-hydroxybutyrate pathways is highly represented in Shark Bay metagenomes while not represented in Highbourne Cay microbial mats or any other mat forming ecosystems investigated to date. Potentially novel aspects of nitrogen cycling were also observed, as well as putative heavy metal cycling (arsenic, mercury, copper and cadmium). Finally, archaea are highly represented in Shark Bay and may have critical roles in overall ecosystem function in these modern microbial mats.

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“…Due to the restricted flow of seawater into Hamelin Pool, Shark Bay, Australia and the high net evaporation rates, the salinity of the surface water in Hamelin Pool is twice that of normal seawater and the living stromatolites are partially submerged in this hypersaline environment. Halococcus hamelinensis (Goh et al 2006 ; Burns et al 2012 ) was isolated from a stromatolite in Hamelin pool. Although Hcc.…”

Section: Discussionmentioning

confidence: 99%

Thermophilic and halophilic β-agarase from a halophilic archaeon Halococcus sp. 197A

et al. 2013

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An agar-degrading archaeon Halococcus sp. 197A was isolated from a solar salt sample. The agarase was purified by hydrophobic column chromatography using a column of TOYOPEARL Phenyl-650 M. The molecular mass of the purified enzyme, designated as Aga-HC, was ~55 kDa on both SDS-PAGE and gel-filtration chromatography. Aga-HC released degradation products in the order of neoagarohexose, neoagarotetraose and small quantity of neoagarobiose, indicating that Aga-HC was a β-type agarase. Aga-HC showed a salt requirement for both stability and activity, being active from 0.3 M NaCl, with maximal activity at 3.5 M NaCl. KCl supported similar activities as NaCl up to 3.5 M, and LiCl up to 2.5 M. These monovalent salts could not be substituted by 3.5 M divalent cations, CaCl2 or MgCl2. The optimal pH was 6.0. Aga-HC was thermophilic, with optimum temperature of 70 °C. Aga-HC retained approximately 90 % of the initial activity after incubation for 1 hour at 65–80 °C, and retained 50 % activity after 1 hour at 95 °C. In the presence of additional 10 mM CaCl2, approximately 17 % remaining activity was detected after 30 min at 100 °C. This is the first report on agarase purified from Archaea.Electronic supplementary materialThe online version of this article (doi:10.1007/s00792-013-0575-z) contains supplementary material, which is available to authorized users.

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Genome Sequence of the Halophilic Archaeon Halococcus hamelinensis

Abstract: Halococcus hamelinensis was isolated from hypersaline stromatolites in Shark Bay, Australia. Here we report the genome sequence (3,133,046 bp) of H. hamelinensis, which provides insights into the ecology, evolution, and adaptation of this novel microorganism.

Cited by 15 publications

References 15 publications

Properties of Halococcus salifodinae, an Isolate from Permian Rock Salt Deposits, Compared with Halococci from Surface Waters

Properties of Halococcus salifodinae, an Isolate from Permian Rock Salt Deposits, Compared with Halococci from Surface Waters

Unravelling core microbial metabolisms in the hypersaline microbial mats of Shark Bay using high-throughput metagenomics

Thermophilic and halophilic β-agarase from a halophilic archaeon Halococcus sp. 197A

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