Genome Sequence of the Marine Bacterium Vibrio campbellii DS40M4, Isolated from Open Ocean Water

Dias, Glaecir Roseni Mundstock; Thompson, Cristiane C.; Fishman, Brian; Naka, Hiroaki; Haygood, Margo G.; Crosa, Jorge H.; Thompson, Fabiano L.

doi:10.1128/jb.06583-11

Cited by 19 publications

(16 citation statements)

References 9 publications

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“…YM5-799. In addition to the cyclic triscatecholate siderophores, a linear (non-cyclized) triscatecholate siderophore, trivanchrobactin, was reported from the marine isolate Vibrio campbelli DS40M4 [18], [19]. Trivanchrobactin is a linear trimeric ester of the previously reported vanchrobactin, 2,3-DHB-D-Arg-L-Ser [20].…”

Section: Introductionmentioning

confidence: 93%

Turnerbactin, a Novel Triscatecholate Siderophore from the Shipworm Endosymbiont Teredinibacter turnerae T7901

et al. 2013

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Shipworms are marine bivalve mollusks (Family Teredinidae) that use wood for shelter and food. They harbor a group of closely related, yet phylogenetically distinct, bacterial endosymbionts in bacteriocytes located in the gills. This endosymbiotic community is believed to support the host's nutrition in multiple ways, through the production of cellulolytic enzymes and the fixation of nitrogen. The genome of the shipworm endosymbiont Teredinibacter turnerae T7901 was recently sequenced and in addition to the potential for cellulolytic enzymes and diazotrophy, the genome also revealed a rich potential for secondary metabolites. With nine distinct biosynthetic gene clusters, nearly 7% of the genome is dedicated to secondary metabolites. Bioinformatic analyses predict that one of the gene clusters is responsible for the production of a catecholate siderophore. Here we describe this gene cluster in detail and present the siderophore product from this cluster. Genes similar to the entCEBA genes of enterobactin biosynthesis involved in the production and activation of dihydroxybenzoic acid (DHB) are present in this cluster, as well as a two-module non-ribosomal peptide synthetase (NRPS). A novel triscatecholate siderophore, turnerbactin, was isolated from the supernatant of iron-limited T. turnerae T7901 cultures. Turnerbactin is a trimer of N-(2,3-DHB)-L-Orn-L-Ser with the three monomeric units linked by Ser ester linkages. A monomer, dimer, dehydrated dimer, and dehydrated trimer of 2,3-DHB-L-Orn-L-Ser were also found in the supernatant. A link between the gene cluster and siderophore product was made by constructing a NRPS mutant, TtAH03. Siderophores could not be detected in cultures of TtAH03 by HPLC analysis and Fe-binding activity of culture supernatant was significantly reduced. Regulation of the pathway by iron is supported by identification of putative Fur box sequences and observation of increased Fe-binding activity under iron restriction. Evidence of a turnerbactin fragment was found in shipworm extracts, suggesting the production of turnerbactin in the symbiosis.

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Section: Introductionmentioning

confidence: 93%

Turnerbactin, a Novel Triscatecholate Siderophore from the Shipworm Endosymbiont Teredinibacter turnerae T7901

et al. 2013

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“…Phylogenetic analyses showed that the PR gene in this strain, and also the associated crt genes encoding the enzymes for synthesis of the PR chromophore retinal, were acquired through lateral gene transfer from outside of the family Vibrionaceae and even outside of the Gammaproteobacteria (Gómez‐Consarnau et al ., ). In addition to AND4, PR is also found in three closely related V. campbellii strains (Gómez‐Consarnau et al ., ; Amaral et al ., ; Dias et al ., ; Wang et al ., ). At present, most currently available genome‐sequences from members of the genus Vibrio pertain to disease‐associated strains (particularly V. cholerae ), and few belong to vibrios obtained directly from seawater.…”

Section: Introductionmentioning

confidence: 99%

Regulation of proteorhodopsin gene expression by nutrient limitation in the marine bacterium Vibrio sp. AND4

Akram

Palovaara

Forsberg

et al. 2013

Environmental Microbiology

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Proteorhodopsin (PR), a ubiquitous membrane photoprotein in marine environments, acts as a light-driven proton pump and can provide energy for bacterial cellular metabolism. However, knowledge of factors that regulate PR gene expression in different bacteria remains strongly limited. Here, experiments with Vibrio sp. AND4 showed that PR phototrophy promoted survival only in cells from stationary phase and not in actively growing cells. PR gene expression was tightly regulated, with very low values in exponential phase, a pronounced peak at the exponential/stationary phase intersection, and a marked decline in stationary phase. Thus, PR gene expression at the entry into stationary phase preceded, and could therefore largely explain, the stationary phase light-induced survival response in AND4. Further experiments revealed nutrient limitation, not light exposure, regulated this differential PR expression. Screening of available marine vibrios showed that the PR gene, and thus the potential for PR phototrophy, is found in at least three different clusters in the genus Vibrio. In an ecological context, our findings suggest that some PR-containing bacteria adapted to the exploitation of nutrient-rich micro-environments rely on a phase of relatively slowly declining resources to mount a cellular response preparing them for adverse conditions dispersed in the water column.

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“…, ; Sung, Pineda, Macrae, Sorgeloos & Bossier ; Haldar, Chatterjee, Sugimoto, Das, Chowdhury, Hinenoya, Asakura & Yamasaki ). Now, V. campbellii has become another important pathogen, and attracts more and more interests (Amarala, Silvaa, Santosa, Diasa, Lopesb, Edwardsa, Thompsond & Thompsona ; Dias, Thompson, Fishman, Naka, Haygood, Crosa & Thompson ).…”

Section: Introductionmentioning

confidence: 99%

Isolation and identification ofVibrio campbelliias a bacterial pathogen for luminous vibriosis ofLitopenaeus vannamei

et al. 2013

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Outbreak of luminescent disease was reported from Litopenaeus vannamei shrimp farms in Zhangpu County, Southern China during May–July 2011. The clinical signs included fluorescent, less food consumption and high mortality. Bacteria were isolated from the infected shrimps. The pathogen, a luminescent bacterium named VH1 was identified as Vibrio campbellii based on MLSA analysis (16S rDNA, rpoD and toxR). The haemolysin (hly) gene specific in V. campbellii was detected in strain VH1. Pathogenicity test using immersion infection confirmed that strain VH1 was virulent to L. vannamei postlarvae and juveniles, and the LC50 value was 1.55 × 106 CFU mL−1 and 1.7 × 106 CFU mL−1 respectively.

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Genome Sequence of the Marine Bacterium Vibrio campbellii DS40M4, Isolated from Open Ocean Water

Cited by 19 publications

References 9 publications

Turnerbactin, a Novel Triscatecholate Siderophore from the Shipworm Endosymbiont Teredinibacter turnerae T7901

Turnerbactin, a Novel Triscatecholate Siderophore from the Shipworm Endosymbiont Teredinibacter turnerae T7901

Regulation of proteorhodopsin gene expression by nutrient limitation in the marine bacterium Vibrio sp. AND4

Isolation and identification ofVibrio campbelliias a bacterial pathogen for luminous vibriosis ofLitopenaeus vannamei

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