Genome Sequences of 11 Human Vaginal
            <i>Actinobacteria</i>
            Strains

Lewis, Amanda L.; Deitzler, Grace E.; Ruiz, Maria J.; Weimer, Cory; Park, So Eun; Robinson, Linda J.; Hallsworth-Pepin, Kymberlie; Wollam, Aye; Mitreva, Makedonka; Lewis, Warren G.

doi:10.1128/genomea.00887-16

Cited by 7 publications

(4 citation statements)

References 18 publications

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“…Sm R isolate of G . vaginalis JCP8151B [ 31 , 81 ], hereafter referred to simply as G . vaginalis , was cultured in NYCIII media containing 10% heat-inactivated horse serum at 37°C in an anaerobic chamber unless stated otherwise.…”

Section: Methodsmentioning

confidence: 99%

“…For plasmid maintenance, ampicillin was used at 100 μg/mL and chloramphenicol at 20 μg/mL. Sm R isolate of G. vaginalis JCP8151B [31,81], hereafter referred to simply as G. vaginalis, was cultured in NYCIII media containing 10% heat-inactivated horse serum at 37˚C in an anaerobic chamber unless stated otherwise. F. nucleatum strains ATCC25586, ATCC23726, and ATCC10953 were obtained from ATCC (Manassas, VA, USA).…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

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Glycan cross-feeding supports mutualism between Fusobacterium and the vaginal microbiota

et al. 2020

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Women with bacterial vaginosis (BV), an imbalance of the vaginal microbiome, are more likely to be colonized by potential pathogens such as Fusobacterium nucleatum, a bacterium linked with intrauterine infection and preterm birth. However, the conditions and mechanisms supporting pathogen colonization during vaginal dysbiosis remain obscure. We demonstrate that sialidase activity, a diagnostic feature of BV, promoted F. nucleatum foraging and growth on mammalian sialoglycans, a nutrient resource that was otherwise inaccessible because of the lack of endogenous F. nucleatum sialidase. In mice with sialidase-producing vaginal microbiotas, mutant F. nucleatum unable to consume sialic acids was impaired in vaginal colonization. These experiments in mice also led to the discovery that F. nucleatum may also "give back" to the community by reinforcing sialidase activity, a biochemical feature of human dysbiosis. Using human vaginal bacterial communities, we show that F. nucleatum supported robust outgrowth of Gardnerella vaginalis, a major sialidase producer and one of the most abundant organisms in BV. These results illustrate that mutually beneficial relationships between vaginal bacteria support pathogen colonization and may help maintain features of dysbiosis. These findings challenge the simplistic dogma that the mere absence of "healthy" lactobacilli is the sole mechanism that creates a permissive environment for pathogens during vaginal dysbiosis. Given the ubiquity of F. nucleatum in the human mouth, these studies also suggest a possible mechanism underlying links between vaginal dysbiosis and oral sex.

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Section: Methodsmentioning

confidence: 99%

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Glycan cross-feeding supports mutualism between Fusobacterium and the vaginal microbiota

et al. 2020

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“…Many different species of anaerobes, particularly those residing on mucosal surfaces, can play a role in human infections ( Jousimies-Somer 2002 ; Horz et al, 2005 ). For example, species from the Fusobacteria, Bacteroidetes, Actinobacteria, and Firmicutes phyla that are found in the oral cavity have been implicated in tooth and periodontal infections ( Newman 1984 ; Garrett and Onderdonk 2010 ; Oh et al, 2015 ), while bacterial vaginosis has been linked to Bacteroidetes and Actinobacteria species ( Taylor et al, 1982 ; Eschenbach 1993 ; Africa et al, 2014 ; Lewis et al, 2016 ). Intra-abdominal infections (IAI) can be caused by a wide variety of opportunistic pathogens that bloom during gut dysbiosis, and examples of such microbes include C. difficile ( Leffler and Lamont 2015 ; Czepiel et al, 2019 ) and species in the Proteobacteria phylum, most notably members of the Enterobacteriaceae family ( de Ruiter et al, 2009 ; Blot et al, 2012 ; Bäumler and Sperandio 2016 ).…”

Section: Clinical Contexts Of Oxygen Availabilitymentioning

confidence: 99%

Oxygen and Metabolism: Digesting Determinants of Antibiotic Susceptibility in the Gut

et al. 2020

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Summary Microbial metabolism is a major determinant of antibiotic susceptibility. Environmental conditions that modify metabolism, notably oxygen availability and redox potential, can directly fine-tune susceptibility to antibiotics. Despite this, relatively few studies have discussed these modifications within the gastrointestinal tract and their implication on in vivo drug activity and the off-target effects of antibiotics in the gut. In this review, we discuss the environmental and biogeographical complexity of the gastrointestinal tract in regard to oxygen availability and redox potential, addressing how the heterogeneity of gut microhabitats may modify antibiotic activity in vivo . We contextualize the current literature surrounding oxygen availability and antibiotic efficacy and discuss empirical treatments. We end by discussing predicted patterns of antibiotic activity in prominent microbiome taxa, given gut heterogeneity, oxygen availability, and polymicrobial interactions. We also propose additional work required to fully elucidate the role of oxygen metabolism on antibiotic susceptibility in the context of the gut.

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“…For plasmid maintenance, ampicillin was used at 100 μg/mL and chloramphenicol at 20 μg/mL. Gardnerella vaginalis JCP8151B (Lewis et al, 2016;Lewis et al, 2013), hereafter simply referred to simply as G. vaginalis, was cultured in NYC-III media containing 10% heat inactivated horse serum at 37°C in an anaerobic chamber. Fusobacterium nucleatum strains ATCC25586, ATCC23726 and ATCC10953 were obtained from ATCC.…”

Section: Bacterial Strains and Growth Conditionsmentioning

confidence: 99%

Glycan cross-feeding drives mutualism betweenFusobacteriumand the vaginal microbiota

Agarwal

Robinson

Perry

et al. 2018

Preprint

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Dysbiosis of the vaginal microbiome is associated with vaginal colonization by potential pathogens including Fusobacterium nucleatum, a bacterium linked with intrauterine infection and preterm birth. However, mechanisms by which such pathogens gain a foothold in the dysbiotic vagina remain obscure. Here we demonstrate that sialidase activity, a biochemical marker of vaginal dysbiosis, promoted F. nucleatum foraging on mammalian sialoglycans, an otherwise inaccessible resource. In mice with sialidase-positive vaginal microbiomes, mutant F. nucleatum unable to consume sialic acids displayed impaired colonization. Furthermore, community-and co-culture experiments showed that F. nucleatum did not simply take advantage of sialidase-positive bacteria, but also "gave back" to the community, supporting robust outgrowth of sialidase-producers, including Gardnerella vaginalis. These results illustrate that mutualistic relationships between vaginal bacteria support pathogen colonization and reinforce dysbiosis, adding complexity to the simplistic dogma that the mere absence of "healthy" lactobacilli is what creates a permissive environment for pathogens during dysbiosis.

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Genome Sequences of 11 Human Vaginal Actinobacteria Strains

Cited by 7 publications

References 18 publications

Glycan cross-feeding supports mutualism between Fusobacterium and the vaginal microbiota

Glycan cross-feeding supports mutualism between Fusobacterium and the vaginal microbiota

Oxygen and Metabolism: Digesting Determinants of Antibiotic Susceptibility in the Gut

Glycan cross-feeding drives mutualism betweenFusobacteriumand the vaginal microbiota

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