Genome Sequences of Bacillus sporothermodurans Strains Isolated from Ultra-High-Temperature Milk

Owusu-Darko, Rodney; Allam, Mushal; Oliveira, Sı́lvia Dias de; Ferreira, Carlos Alexandre Sanchez; Grover, Sunita; Mtshali, Senzo; Ismail, Arshad; Mallappa, Rashmi Hogarehalli; Tabit, Frederick Tawi; Buys, E.M.

doi:10.1128/mra.00145-19

Cited by 4 publications

(5 citation statements)

References 13 publications

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“…Bacillus sporothermodurans PL01 is a bacterium which produces highly temperature-resistant endospores [48]. In contrast to green algae C. vulgaris, the photometer signal of PL01 increases from a cell density of 2•10 7 mL −1 on, whereas the autofluorescence intensity can be measured beginning from a cell density of 2•10 8 mL −1 (Figure 10D,H).…”

Section: Photo-/fluorimetric Sensor Calibration For Different Microbial Culturesmentioning

confidence: 99%

Droplet-Based Screening for the Investigation of Microbial Nonlinear Dose–Response Characteristics System, Background and Examples

Cao

Richter

Kastl³

et al. 2020

Micromachines

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Droplet-based microfluidics is a versatile tool to reveal the dose–response relationship of different effectors on the microbial proliferation. Traditional readout parameter is the temporal development of the cell density for different effector concentrations. To determine nonlinear or unconventional dose–response relationships, data with high temporal resolution and dense concentration graduation are essential. If microorganisms with slow microbial growth kinetics are investigated, a sterile and evaporation-free long-term incubation technique is required. Here, we present a modular droplet-based screening system which was developed to solve these issues. Beside relevant technical aspects of the developed modules, the procedural workflow, and exemplary dose–response data for 1D and 2D dose–response screenings are presented.

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Section: Photo-/fluorimetric Sensor Calibration For Different Microbial Culturesmentioning

confidence: 99%

Droplet-Based Screening for the Investigation of Microbial Nonlinear Dose–Response Characteristics System, Background and Examples

Cao

Richter

Kastl³

et al. 2020

Micromachines

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“…Bacillus sporothermodurans strains SAD, SA01, BR12, and DSM 10599, as well as B. oleronius DSM 9356, were sequenced and annotated as previously described [15,16]. Subsequent analyses in this study are based on the strains mentioned above.…”

Section: Genome Sequencing and Analysismentioning

confidence: 99%

“…Differences in heat resistance are thought to be clonal, which explains the growing interest in the identification of evolutionary associations and the understanding of its high heat resistance. In this study, the whole genomes of B. sporothermodurans strains isolated from UHT milk from South Africa and Brazil, the type strain, and B. oleronius , its closest phylogenetic neighbor, were sequenced [ 14 , 15 ], with the aim of comparing proteins involved in heat resistance between B . sporothermodurans and other Bacillus species to better understand this phenotype in B .…”

Section: Introductionmentioning

confidence: 99%

Comparative Genome Analysis of Bacillus sporothermodurans with Its Closest Phylogenetic Neighbor, Bacillus oleronius, and Bacillus cereus and Bacillus subtilis Groups

et al. 2020

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Bacillus sporothermodurans currently possesses one of the most highly heat-resistant spores (HRS), which can withstand ultra-high temperature (UHT) processing. Determination of multiple whole genome sequences of B. sporothermodurans provided an opportunity to perform the first comparative genome analysis between strains and with B. oleronius, B. cereus, and B. subtilis groups. In this study, five whole genome sequences of B. sporothermodurans strains, including those belonging to the HRS clone (SAD and BR12) normally isolated from UHT milk, were compared with the aforementioned Bacillus species for gene clusters responsible for heat resistance. In the phylogenomic analysis, B. sporothermodurans, with its closest phylogenetic neighbor, B. oleronius, clustered with B. thermoamylovorans and B. thermotolerans. Heat shock proteins GrpE, GroES, GroEL, and DnaK presented identical sequences for all B. sporothermodurans strains, indicating that differences in functional efficiency are not involved in the thermal resistance variations. However, comparing all species evaluated, B. sporothermodurans exhibited a different gene configuration in the chromosomal region of the heat shock protein GrpE. Furthermore, only B. sporothermodurans strains presented the stage II sporulation protein P gene located in this region. Multisequence alignment and phylogenetic analysis of the ClpB protein showed differences for HRS and non-HRS strains. The study identified ClpC, ClpE, and ClpX as the three ATPases putatively involved in protein disaggregation in B. sporothermodurans. Bacillussporothermodurans exhibits high homology with other Bacillus species in the DnaK, DnaJ, GroEL, and GroES cluster of genes involved in heat resistance. The data presented here pave the way to select and evaluate the phenotypic effects of genes putatively involved in heat resistance.

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“…Circular chromosomes were rotated to dnak as starting point. Possible contamination of the assemblies was screened using the ContEst16S tool [ 22 ]. The genome quality was assessed with the PATRIC quality tool [ 21 ].…”

Section: Methodsmentioning

confidence: 99%

“…It currently provides the best possibility to clarify genomic relationships with the highest discriminatory power [ 21 ]. At the time of writing, the genome of the H. vini type strain was not published and the genome sequences of only five H. sporothermodurans strains were available, i.e., those of the type strain DSM 10599 T , strain BR3 (also named BR12), strain SAD, strain SA01 and B4102 [ 15 , 22 ]. Their assemblies consist of 100–800 contigs, which is a relatively high number when compared to other bacterial species.…”

Section: Introductionmentioning

confidence: 99%

Taxonomic Evaluation of the Heyndrickxia (Basonym Bacillus) sporothermodurans Group (H. sporothermodurans, H. vini, H. oleronia) Based on Whole Genome Sequences

et al. 2021

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The genetic heterogeneity of Heyndrickxia sporothermodurans (formerly Bacillussporothermodurans) was evaluated using whole genome sequencing. The genomes of 29 previously identified Heyndrickxiasporothermodurans and two Heyndrickxia vini strains isolated from ultra-high-temperature (UHT)-treated milk were sequenced by short-read (Illumina) sequencing. After sequence analysis, the two H. vini strains could be reclassified as H. sporothermodurans. In addition, the genomes of the H.sporothermodurans type strain (DSM 10599T) and the closest phylogenetic neighbors Heyndrickxiaoleronia (DSM 9356T) and Heyndrickxia vini (JCM 19841T) were also sequenced using both long (MinION) and short-read (Illumina) sequencing. By hybrid sequence assembly, the genome of the H. sporothermodurans type strain was enlarged by 15% relative to the short-read assembly. This noticeable increase was probably due to numerous mobile elements in the genome that are presumptively related to spore heat tolerance. Phylogenetic studies based on 16S rDNA gene sequence, core genome, single-nucleotide polymorphisms and ANI/dDDH, showed that H. vini is highly related to H. sporothermodurans. When examining the genome sequences of all H.sporothermodurans strains from this study, together with 4 H. sporothermodurans genomes available in the GenBank database, the majority of the 36 strains examined occurred in a clonal lineage with less than 100 SNPs. These data substantiate previous reports on the existence and spread of a genetically highly homogenous and heat resistant spore clone, i.e., the HRS-clone.

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Genome Sequences of Bacillus sporothermodurans Strains Isolated from Ultra-High-Temperature Milk

Cited by 4 publications

References 13 publications

Droplet-Based Screening for the Investigation of Microbial Nonlinear Dose–Response Characteristics System, Background and Examples

Droplet-Based Screening for the Investigation of Microbial Nonlinear Dose–Response Characteristics System, Background and Examples

Comparative Genome Analysis of Bacillus sporothermodurans with Its Closest Phylogenetic Neighbor, Bacillus oleronius, and Bacillus cereus and Bacillus subtilis Groups

Taxonomic Evaluation of the Heyndrickxia (Basonym Bacillus) sporothermodurans Group (H. sporothermodurans, H. vini, H. oleronia) Based on Whole Genome Sequences

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