Genome Sequences of Five Clinical Isolates of
            <i>Klebsiella pneumoniae</i>

López, Lázaro; Rusconi, Brigida; Gildersleeve, Heidi; Qi, Chao; McLaughlin, Milena M.; Scheetz, Marc H.; Seshu, J.; Eppinger, Mark

doi:10.1128/genomea.00040-16

Cited by 7 publications

(6 citation statements)

References 26 publications

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“…DNA preparation for next-generation sequencing (NGS). Sequencing of genomes was performed as previously described (85). Briefly, total genomic DNA (gDNA) from each strain was obtained through phenol-chloroform extraction and checked for purity spectrophotometrically using a NanoDrop spectrophotometer (Thermo Fisher).…”

Section: Methodsmentioning

confidence: 99%

Borrelia Host Adaptation Protein (BadP) Is Required for the Colonization of a Mammalian Host by the Agent of Lyme Disease

et al. 2018

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(), the agent of Lyme disease (LD), uses host-derived signals to modulate gene expression during the vector and mammalian phases of infection. Microarray analysis of mutants lacking orrelia host-aptation egulator (BadR) revealed down-regulation of genes encoding enzymes whose role in the patho-physiology of is unknown. Immunoblot analysis of the mutants confirmed reduced levels of these enzymes and one of these enzymes encoded by, shares homology to prokaryotic magnesium chelatase and Lon-type proteases. BB0086 levels were higher in under conditions mimicking fed ticks. Mutants lacking had no apparent growth defect but were incapable of colonizing immunocompetent C3H/HeN and immunodeficient SCID mice. Immunoblot analysis revealed reduced levels of proteins critical for adaptation of to the mammalian host such as OspC, DbpA and BBK32. Both RpoS and BosR - key regulators of gene expression in - were downregulated in the mutants. Therefore, we designated BB0086 as orrelia host-aptation rotein (BadP). Unlike mutants, the control strains established infection in C3H/HeN mice at 4 days post infection, indicating an early colonization defect in mutants due to reduced levels of lipoproteins/regulators critical for initial stages of infection. However, mutants survived within dialysis membrane chambers (DMCs) implanted within rat peritoneal cavity but unlike the control strains did not display complete switching of OspA to OspC suggesting incomplete adaptation to mammalian phase of infection. These findings have opened a novel regulatory mechanism, which impacts the virulence potential of.

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Section: Methodsmentioning

confidence: 99%

Borrelia Host Adaptation Protein (BadP) Is Required for the Colonization of a Mammalian Host by the Agent of Lyme Disease

et al. 2018

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“…; Lopez et al . ; Zhou et al . ), as well as the epidemiology of K. pneumoniae infections (Munoz‐Price et al .…”

Section: Introductionmentioning

confidence: 99%

“…Attributable mortality associated with multidrug resistance (specifically carbapenem resistance) is as high as 50% (Borer et al 2009). Despite a focus in recent years on K. pneumoniae genomics (Kumar et al 2011;Chen et al 2014;Deleo et al 2014;Holt et al 2015;Lopez et al 2016;Zhou et al 2016), as well as the epidemiology of K. pneumoniae infections (Munoz-Price et al 2013;Doi and Paterson 2015), there has been little attention paid to this pathogen's metabolism.…”

Section: Introductionmentioning

confidence: 99%

Expanding theKlebsiella pneumoniaevolatile metabolome using advanced analytical instrumentation for the detection of novel metabolites

et al. 2017

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“…Sequencing of genomes was performed as previously described [41,100]. Briefly, total genomic DNA from each strain was obtained through phenol chloroform extraction and checked for purity spectrophotometrically using NanoDrop microvolume spectrophotometer (Thermo-Fisher).…”

Section: Dna Sequencing For Ngsmentioning

confidence: 99%

Borrelia peptidoglycan interacting Protein (BpiP) contributes to the fitness of Borrelia burgdorferi against host-derived factors and influences virulence in mouse models of Lyme disease

et al. 2021

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The Peptidoglycan (PG) cell wall of the Lyme disease (LD) spirochete, Borrelia burgdorferi (Bb), contributes to structural and morphological integrity of Bb; is a persistent antigen in LD patients; and has a unique pentapeptide with L-Ornithine as the third amino acid that cross-links its glycan polymers. A borrelial homolog (BB_0167) interacted specifically with borrelilal PG via its peptidoglycan interacting motif (MHELSEKRARAIGNYL); was localized to the protoplasmic cylinder of Bb; and was designated as Borrelia peptidoglycan interacting Protein (BpiP). A bpiP mutant displayed no defect under in vitro growth conditions with similar levels of several virulence-related proteins. However, the burden of bpiP mutant in C3H/HeN mice at day 14, 28 and 62 post-infection was significantly lower compared to control strains. No viable bpiP mutant was re-isolated from any tissues at day 62 post-infection although bpiP mutant was able to colonize immunodeficient SCID at day 28 post-infection. Acquisition or transmission of bpiP mutant by Ixodes scapularis larvae or nymphs respectively, from and to mice, was significantly lower compared to control strains. Further analysis of bpiP mutant revealed increased sensitivity to vancomycin, osmotic stress, lysosomal extracts, human antimicrobial peptide cathelicidin-LL37, complement-dependent killing in the presence of day 14 post-infection mouse serum and increased internalization of CFSC-labeled bpiP mutant by macrophages and dendritic cells compared to control strains. These studies demonstrate the importance of accessory protein/s involved in sustaining integrity of PG and cell envelope during different phases of Bb infection.

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Genome Sequences of Five Clinical Isolates of Klebsiella pneumoniae

Cited by 7 publications

References 26 publications

Borrelia Host Adaptation Protein (BadP) Is Required for the Colonization of a Mammalian Host by the Agent of Lyme Disease

Borrelia Host Adaptation Protein (BadP) Is Required for the Colonization of a Mammalian Host by the Agent of Lyme Disease

Expanding theKlebsiella pneumoniaevolatile metabolome using advanced analytical instrumentation for the detection of novel metabolites

Borrelia peptidoglycan interacting Protein (BpiP) contributes to the fitness of Borrelia burgdorferi against host-derived factors and influences virulence in mouse models of Lyme disease

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