Genome Sequences of Five Indian Canine Rabies Virus Isolates Obtained Using Oxford Nanopore Technologies Sequencing

Kumar, Ankeet; Nath, Sujith S; Sudarshan, Ashwin Sridhar; Iyer, Vinithra; Jadeja, Niti B.; Panchamia, Neha; Banerji, Indrakshi; Vanak, Abi Tamim; Tatu, Utpal

doi:10.1128/mra.01246-21

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“…Despite being endemic for canine rabies and accounting for the highest burden of human deaths due to rabies world-wide, there are limited studies focusing on the molecular epidemiology of RABV from India. Most of these reports are based on single gene sequencing [15][16][17][18][19][20][21][22][23][24] , with only a few reports of complete RABV genomes [35][36][37] . This report with 20 complete genomes, recovered directly from archived human and canine brain samples, provides an important starting point for analyzing the evolution and spread of RABV in India.…”

Section: Discussionmentioning

confidence: 99%

Whole genome sequencing of rabies virus from archived human and canine brain tissues from southern India using Nanopore technology

Mani

Harsha

Prasad

et al. 2023

Preprint

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Rabies, a fatal zoonotic viral encephalitis kills tens of thousands of people each year in India, representing one third of the estimated global rabies burden. A detailed understanding of the molecular epidemiology of the Rabies lyssavirus (RABV) in India is limited due to the low availability of sequences from the country. In this study we examined the feasibility of direct sequencing of clinical samples (archived human and canine brain tissues, 10 each), for detection and recovery of whole genome sequences of RABV, on the MinION – single molecule nanopore sequencing device. Using an amplicon-based approach, complete RABV genomes were recovered from all the 20 samples, archived from 2003 to 2019, from 4 southern states of India. Phylogenetic analysis revealed that all 20 sequences from this study belonged to the Arctic-like (AL) 1a lineage. Divergence estimation of sequences revealed that all the circulating RABV strains within India are diverged in last 60 years from the previous ancestor. The genetic relatedness and close clustering of the sequences in the study suggests the continued propagation of AL1a in the region, across state boundaries. Genomic surveillance can provide unique insights into rabies spread and persistence that can direct control efforts in India.

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Section: Discussionmentioning

confidence: 99%

Whole genome sequencing of rabies virus from archived human and canine brain tissues from southern India using Nanopore technology

Mani

Harsha

Prasad

et al. 2023

Preprint

View full text Add to dashboard Cite

show abstract

Genome Sequences of Five Indian Canine Rabies Virus Isolates Obtained Using Oxford Nanopore Technologies Sequencing

Abstract: We report five canine rabies virus genome sequences from India that were obtained from brain samples using Oxford Nanopore Technologies sequencing. The sequences will facilitate understanding of the evolution and transmission of rabies.

Cited by 1 publication

References 6 publications

Whole genome sequencing of rabies virus from archived human and canine brain tissues from southern India using Nanopore technology

Whole genome sequencing of rabies virus from archived human and canine brain tissues from southern India using Nanopore technology

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