Genome sequences of five Sitopsis species of Aegilops and the origin of polyploid wheat B subgenome

Li, Linfeng; Zhang, Zhibin; Wang, Zhenhui; Li, Ning; Sha, Yan; Wang, Xinfeng; Ding, Ning; Li, Yang; Zhao, Jing; Wu, Ying; Gong, Lei; Mafessoni, Fabrizio; Levy, Avraham A.; B, Liu

doi:10.1016/j.molp.2021.12.019

Cited by 117 publications

(160 citation statements)

References 98 publications

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“…Preferential transmission of chromosome 2S of Ae. speltoides , proposed to be more closely related to the T. timopheevii G genome than to the B genome of wheat (Li et al, 2022; Rodriguez et al, 2000a), is well documented (King et al, 2018; Tsujimoto and Tsunewaki 1988) although the gene causing the preferential transmission in T. timopheevii appears to be weaker than the gene for preferential transmission in Ae. speltoides .…”

Section: Discussionmentioning

confidence: 99%

Introgression of the Triticum timopheevii genome into wheat detected by chromosome-specific KASP markers

King

Grewal

Othmeni

et al. 2022

Preprint

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Triticum timopheevii (2n=28, AtAtGG) is a tetraploid wild relative species with great potential to increase the genetic diversity of hexaploid wheat Triticum aestivum (2n=42, AABBDD) for various important agronomic traits. A breeding scheme that propagated advanced backcrossed populations of wheat-T. timopheevii introgression lines through further backcrossing and self-fertilisation resulted in the generation of 99 introgression lines (ILs) that carried 309 homozygous segments from the At and G subgenomes of T. timopheevii. These introgressions contained 89 and 74 unique segments from the At and G subgenomes, respectively. These overlapping segments covered 98.9% of the T. timopheevii genome that has now been introgressed into bread wheat cv. Paragon including the entirety of all T. timopheevii chromosomes via varying sized segments except for chromosomes 3At, 4G and 6G. Homozygous ILs contained between one and eight of these introgressions with an average of three per introgression line. These homozygous introgressions were detected through the development of a set of 480 chromosome-specific Kompetitive allele specific PCR (KASP) markers that are well-distributed across the wheat genome. Of these, 149 were developed in this study based on single nucleotide polymorphisms (SNPs) discovered through whole genome sequencing of T. timopheevii. A majority of these KASP markers were also found to be T. timopheevii subgenome specific with 182 detecting At subgenome and 275 detecting G subgenome segments. These markers showed that 98% of the At segments had recombined with the A genome of wheat and 74% of the G genome segments had recombined with the B genome of wheat with the rest recombining with the D genome of wheat. These results were validated through multi-colour in situ hybridisation analysis. Together these homozygous wheat-T. timopheevii ILs and chromosome-specific KASP markers provide an invaluable resource to wheat breeders for trait discovery to combat biotic and abiotic stress factors affecting wheat production due to climate change.

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Section: Discussionmentioning

confidence: 99%

Introgression of the Triticum timopheevii genome into wheat detected by chromosome-specific KASP markers

King

Grewal

Othmeni

et al. 2022

Preprint

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“…However, recent research indicated that Ae. speltoides was not the direct progenitor of the B genome [45]. The comparison of genome sequences of five Aegilops Sitopsis species showed that Ae.…”

Section: Origin Domestication and Evolution Of Tetraploid Wheat 21 Or...mentioning

confidence: 99%

“…The comparison of genome sequences of five Aegilops Sitopsis species showed that Ae. speltoides and the B-subgenome diverged about 4.49 million years BP, which was much earlier than the speciation of tetraploid emmer wheat [45]. The long evolution history after tetraploid wheat generation may result in the high divergence of B genomes between hexaploid and diploid donors, making it difficult to deduce the precise donor/s [46,47].…”

Section: Origin Domestication and Evolution Of Tetraploid Wheat 21 Or...mentioning

confidence: 99%

Improvement and Re-Evolution of Tetraploid Wheat for Global Environmental Challenge and Diversity Consumption Demand

Yang

Zhang

Liu

et al. 2022

IJMS

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Allotetraploid durum wheat is the second most widely cultivated wheat, following hexaploid bread wheat, and is one of the major protein and calorie sources of the human diet. However, durum wheat is encountered with a severe grain yield bottleneck due to the erosion of genetic diversity stemming from long-term domestication and especially modern breeding programs. The improvement of yield and grain quality of durum wheat is crucial when confronted with the increasing global population, changing climate environments, and the non-ignorable increasing incidence of wheat-related disorders. This review summarized the domestication and evolution process and discussed the durum wheat re-evolution attempts performed by global researchers using diploid einkorn, tetraploid emmer wheat, hexaploid wheat (particularly the D-subgenome), etc. In addition, the re-evolution of durum wheat would be promoted by the genetic enrichment process, which could diversify allelic combinations through enhancing chromosome recombination (pentaploid hybridization or pairing of homologous chromosomes gene Ph mutant line induced homoeologous recombination) and environmental adaptability via alien introgressive genes (wide cross or distant hybridization followed by embryo rescue), and modifying target genes or traits by molecular approaches, such as CRISPR/Cas9 or RNA interference (RNAi). A brief discussion of the future perspectives for exploring germplasm for the modern improvement and re-evolution of durum wheat is included.

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“…Wheat ( Triticum aestivum ) is an allohexaploid crop containing three genomes (2n = 6× = 42, AABBDD). The tetraploid T. turgidum (2n = 4× = 28, AABB) was first originated from the progeny of the polyploidization of T. urartu (donor of A genome) and an unknown diploid species of the Sitopsis (donor of B genome) [ 1 ]. Then, the hexaploid wheat was generated from the allopolyploid between T. turdidum and Aegilops tauschii (donor of D genome) about 10,000 years ago [ 1 , 2 ].…”

Section: Introductionmentioning

confidence: 99%

“…The tetraploid T. turgidum (2n = 4× = 28, AABB) was first originated from the progeny of the polyploidization of T. urartu (donor of A genome) and an unknown diploid species of the Sitopsis (donor of B genome) [ 1 ]. Then, the hexaploid wheat was generated from the allopolyploid between T. turdidum and Aegilops tauschii (donor of D genome) about 10,000 years ago [ 1 , 2 ]. Numerous reciprocal or Robertsonian translocations have been detected in global accessions of polyploidy wheat species [ 3 , 4 , 5 ].…”

Section: Introductionmentioning

confidence: 99%

Molecular Cytogenetic Characterization of Novel 1RS.1BL Translocation and Complex Chromosome Translocation Lines with Stripe Rust Resistance

Ren

Tan

et al. 2022

IJMS

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Rye is the most important source for the genetic improvement of wheat. In this study, two stable wheat-rye primary 1RS.1BL translocation lines, RT855-13 and RT855-14, were selected and identified by acid polyacrylamide gel electrophoresis (A-PAGE), co-dominant PCR, and multi-color fluorescence in situ hybridization (MC-FISH) from the progeny of the crossing of the wheat cultivar Mianyang11 and a Chinese rye Weining. When more than two independent, simple reciprocal translocations are involved in a carrier, they are defined as complex chromosome translocations (CCT). The MC-FISH results also indicated that CCT occurred in RT855-13; namely that, besides 1RS.1BL translocation chromosomes, there are other two pairs of balanced reciprocal translocations. It was demonstrated that the interchange between a distal segment of 4B and long arm of 3D occurred in the RT855-13. The novel translocation chromosomes in wheat were recorded as 3DS.4BSDS and 3DL-4BSPS.4BL. Reports about CCT as a genetic resource in plant breeding programs are scarce. Both lines expressed high resistance to Puccinia striiformis f. sp. tritici, which are prevalent in China and are virulent on Yr9, and the CCT line RT855-13 retained better resistance as adult plants compared with RT855-14 in the field. Both lines, especially the CCT line RT855-13, exhibited better agronomic traits than their wheat parent, Mianyang11, indicating that both translocation lines could potentially be used for wheat improvement. The results also indicated that the position effects of CCT can lead to beneficial variations in agronomic and resistant traits, making them a valuable genetic resource to wheat breeding programs.

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Genome sequences of five Sitopsis species of Aegilops and the origin of polyploid wheat B subgenome

Cited by 117 publications

References 98 publications

Introgression of the Triticum timopheevii genome into wheat detected by chromosome-specific KASP markers

Introgression of the Triticum timopheevii genome into wheat detected by chromosome-specific KASP markers

Improvement and Re-Evolution of Tetraploid Wheat for Global Environmental Challenge and Diversity Consumption Demand

Molecular Cytogenetic Characterization of Novel 1RS.1BL Translocation and Complex Chromosome Translocation Lines with Stripe Rust Resistance

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