Genome Sequences of Gordonia Phages Bowser and Schwabeltier

Abstract

Gordonia phages Bowser and Schwabeltier are newly isolated phages infecting Gordonia terrae 3612. Bowser and Schwabeltier have similar siphoviral morphologies and their genomes are related to each other, but not to other phages. Their lysis cassettes are atypically situated among virion tail genes, and Bowser encodes two tyrosine integrases.

Draft Whole-Genome Sequence of Deinococcus sp. UR1, a Putative Novel Species Isolated from an External Stainless Steel Surface in the Canadian Prairies

“…The draft genome sequence was also analyzed for prophage regions with the PHAge Search Tool Enhanced Release (PHASTER) server ( 7 ). PHASTER identified three incomplete prophage regions which shared similarity with recently published Gordonia phage sequences ( 8 , 9 ) and Salmonella phage sequence 118970_sal2 (GenBank accession number NC_031933).…”

Bacteriophages of Gordonia spp. Display a Spectrum of Diversity and Genetic Relationships

“…Sixty phages isolated on Gordonia spp. through enrichment culture or direct plating as part of the Science Education Alliance-Phage Hunters Advancing Genomics and Evolutionary Science (SEA-PHAGES) and the Phage Hunters Integrating Research and Education (PHIRE) programs ( 15 , 18 – 28 ) were used for comparative analysis ( Table 1 ), together with 19 Gordonia phages described previously ( 13 , 16 , 17 ). All but two of the SEA-PHAGES/PHIRE phages were isolated on G. terrae 3612; the other two were isolated on Gordonia neofelifaecis ( 34 ); the 19 previously described phages were isolated on Gordonia spp., including G. terrae , G. rubripertincta , G. malaquae , G. sputi , or G. alkanivorans ( Table 1 ).…”

“…Sixty phages were isolated on Gordonia spp. through enrichment culture or direct plating as part of the SEA-PHAGES and PHIRE programs, and dsDNA was extracted ( 15 , 18 – 28 ). Double-stranded DNA genomes were sequenced using Illumina Mi-Seq and assembled using Newbler and Consed.…”