Genome Sequences of Newcastle Disease Virus Strains from Two Outbreaks in Indonesia

Abstract

The genomes of two newly emerged Newcastle disease virus strains, chicken/Indonesia/Mega/001WJ/2013 and chicken/Indonesia/Cimanglid/002WJ/2015, from disease outbreaks in chickens in Indonesia are reported. Phylogenetic analysis of different genotypes of Newcastle disease virus using the F gene coding sequences suggests that these two strains belong to genotype VII.2, in class II of avian paramyxoviruses.

Indicators of the molecular pathogenesis of virulent Newcastle disease virus in chickens revealed by transcriptomic profiling of spleen

“…Avirulent strains are often used as live vaccines 6 . Phylogenetic analysis of the fusion protein gene of NDVs indicates that circulating strains in Indonesia are belonging to genotype VII.1 with a mean death time (MDT) from 33 and 30 h as their pathogenicity index 9 , 11 , 12 . These findings also indicate that these circulating strains are clinically categorised as velogenic.…”

“…These findings also indicate that these circulating strains are clinically categorised as velogenic. In this study, we have used one of these genotype VII NDV strains in our challenge experiment in order to analyse pathogenesis of this newly emerged NDV 11 . The amino acid composition of cleavage site of the fusion protein and its susceptibility to host trypsin-like proteases plays a big role in pathogenicity, spread of infection, and tissue tropism of NDVs 13 .…”

Indicators of the molecular pathogenesis of virulent Newcastle Disease Virus in chickens revealed by transcriptomic profiling of spleen

“…Avirulent strains are often used as live vaccines 7 . Phylogenetic analysis of the fusion protein gene of NDVs indicates that circulating strains in Indonesia are belonging to genotype VII.2 with a mean death time (MDT) from 60 to 67 hour as their pathogenicity index 10,12,13 . These ndings also indicate that these circulating strains are clinically categorised as neurotropic velogenic.…”

“…These ndings also indicate that these circulating strains are clinically categorised as neurotropic velogenic. In this study, we have used one of these genotype VII NDV strains in our challenge experiment in order to analyse pathogenesis of this newly emerged NDV 12 . The amino acid composition of cleavage site of the fusion protein and its susceptibility to host trypsin-like proteases plays a big role in pathogenicity, spread of infection, and tissue tropism of NDVs 14 .…”

“…Due to existing the neuropathogenic genetic markers in our NDV isolates 10,12,13 , we were expecting to observe neurological lesions and respiratory symptoms as the classical symptoms of virulent NDV strains. Surprisingly, we have detected almost no NDVs in the central nervous system, while massive lymphoid depletion observed in studied chickens.…”