Genome Sequences of Three Atypical Xanthomonas campestris pv. campestris Strains, CN14, CN15, and CN16

Bolot, Stéphanie; Roux, Brice Le; Carrère, Sébastien; Jiang, Bo-Le; Tang, Ji‐Liang; Arlat, Matthieu; Noël, Laurent

doi:10.1128/genomea.00465-13

Cited by 9 publications

(6 citation statements)

References 14 publications

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“…65 % G + C content. The presence of plasmids has only been reported in strains B-1459, CN14, CN15 and CN16 [ 23 , 25 ]. No genomic information is available yet on the pathovar incanae and the non-pathogenic group of X. campestris .…”

Section: Introductionmentioning

confidence: 99%

Genomics and transcriptomics of Xanthomonas campestris species challenge the concept of core type III effectome

et al. 2015

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BackgroundThe bacterial species Xanthomonas campestris infects a wide range of Brassicaceae. Specific pathovars of this species cause black rot (pv. campestris), bacterial blight of stock (pv. incanae) or bacterial leaf spot (pv. raphani).ResultsIn this study, we extended the genomic coverage of the species by sequencing and annotating the genomes of strains from pathovar incanae (CFBP 1606R and CFBP 2527R), pathovar raphani (CFBP 5828R) and a pathovar formerly named barbareae (CFBP 5825R). While comparative analyses identified a large core ORFeome at the species level, the core type III effectome was limited to only three putative type III effectors (XopP, XopF1 and XopAL1). In Xanthomonas, these effector proteins are injected inside the plant cells by the type III secretion system and contribute collectively to virulence. A deep and strand-specific RNA sequencing strategy was adopted in order to experimentally refine genome annotation for strain CFBP 5828R. This approach also allowed the experimental definition of novel ORFs and non-coding RNA transcripts. Using a constitutively active allele of hrpG, a master regulator of the type III secretion system, a HrpG-dependent regulon of 141 genes co-regulated with the type III secretion system was identified. Importantly, all these genes but seven are positively regulated by HrpG and 56 of those encode components of the Hrp type III secretion system and putative effector proteins.ConclusionsThis dataset is an important resource to mine for novel type III effector proteins as well as for bacterial genes which could contribute to pathogenicity of X. campestris.Electronic supplementary materialThe online version of this article (doi:10.1186/s12864-015-2190-0) contains supplementary material, which is available to authorized users.

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Section: Introductionmentioning

confidence: 99%

Genomics and transcriptomics of Xanthomonas campestris species challenge the concept of core type III effectome

et al. 2015

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“…Next to its natural function, xanthan as a highly viscous exopolysaccharide obtained industrial relevance due to its excellent rheological behavior (6, 7). By now, several strains of Xanthomonas campestris have been sequenced with a focus on plant pathogenicity: X. campestris ATCC 33913 (8), X. campestris 8004 (9), X. campestris Xca5 (10), X. campestris CN14, CN15, and CN16 (11), X. campestris 17 (12), as well as X. campestris CFBP 1869 and CFBP 5817 (13). Genome sequencing approaches were also performed with a focus on xanthan production: X. campestris B100 (14), X. campestris JX (15), and X. campestris ATCC 13951 (16).…”

Section: Genome Announcementmentioning

confidence: 99%

Draft Genome Sequence of the Xanthan Producer Xanthomonas campestris LMG 8031

2016

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“…Genomic studies have resulted in the identification of several genes leading to a better understanding of Xcc pathogenicity mechanisms . Moreover, the determination of the whole genome sequences of four X. campestris (pathovar campestris : 8004, ATCC33913, B100; pathovar raphani : 756C) and the recent announced sequenced genomes of six strains (pathovar campestris : Xca5, JX, B‐1459, CN14, CN15, and CN16) have expanded insights into these molecular mechanisms. Although the complete genome sequencing of Xcc strains has revealed a high amount of information, approximately one‐third of the protein‐coding genes do not have an assigned function .…”

Section: Introductionmentioning

confidence: 99%

Pan Proteome of Xanthomonas campestris pv. campestris Isolates Contrasting in Virulence

et al. 2019

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Fully sequenced genomes of Xanthomonas campestris pv. campestris (Xcc) strains are reported. However, intra‐pathovar differences are still intriguing and far from clear. In this work, the contrasting virulence between two isolates of Xcc ‐ Xcc51 (more virulent) and XccY21 (less virulent) is evaluated by determining their pan proteome profiles. The bacteria are grown in NYG and XVM1 (optimal for induction of hrp regulon) broths and collected at the max‐exponential growth phase. Shotgun proteomics reveals a total of 329 proteins when Xcc isolates are grown in XVM1. A comparison of both profiles reveals 47 proteins with significant abundance fluctuations, out of which, 39 show an increased abundance in Xcc51 and are mainly involved in virulence/adaptation mechanisms, genetic information processing, and membrane receptor/iron transport systems, such as BfeA, BtuB, Cap, Clp, Dcp, FyuA, GroEs, HpaG, Tig, and OmpP6. Several differential proteins are further analyzed by qRT‐PCR, which reveals a similar expression pattern to the protein abundance. The data shed light on the complex Xcc pathogenicity mechanisms and point out a set of proteins related to the higher virulence of Xcc51. This information is essential for the development of more efficient strategies aiming at the control of black rot disease.

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Genome Sequences of Three Atypical Xanthomonas campestris pv. campestris Strains, CN14, CN15, and CN16

Cited by 9 publications

References 14 publications

Genomics and transcriptomics of Xanthomonas campestris species challenge the concept of core type III effectome

Genomics and transcriptomics of Xanthomonas campestris species challenge the concept of core type III effectome

Draft Genome Sequence of the Xanthan Producer Xanthomonas campestris LMG 8031

Pan Proteome of Xanthomonas campestris pv. campestris Isolates Contrasting in Virulence

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