Genome Sequencing and Analysis of the Filamentous Fungus
            <i>Penicillium sclerotiorum</i>
            113, Isolated after Hurricane Sandy

Yin, Guohua; Zhang, Yuliang; Pennerman, Kayla K.; Hua, Sui Sheng T.; Huang, Qixing; Guo, Anping; Liu, Zhixin; Bennett, Joan W.

doi:10.1128/genomea.01153-16

Cited by 4 publications

(3 citation statements)

References 12 publications

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“…sclerotiorum 113 (labeled as 113) was a strain isolated from a home in Manasquan, NJ, flooded with marine water after Hurricane Sandy in 2013, renamed as P . maximae and deposited in NCBI with accession number MJCA01000000 [ 47 , 48 ]. Fresh spores (final concentration at 10 4 /mL) of these three strains were inoculated in potato dextrose broth and incubated with shaking at 200 rpm at 25 °C for seven days in the dark.…”

Section: Methodsmentioning

confidence: 99%

Genomic Analyses of Penicillium Species Have Revealed Patulin and Citrinin Gene Clusters and Novel Loci Involved in Oxylipin Production

Yin

Pennerman

Jurick

et al. 2021

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Blue mold of apple is caused by several different Penicillium species, among which P. expansum and P. solitum are the most frequently isolated. P. expansum is the most aggressive species, and P. solitum is very weak when infecting apple fruit during storage. In this study, we report complete genomic analyses of three different Penicillium species: P. expansum R21 and P. crustosum NJ1, isolated from stored apple fruit; and P. maximae 113, isolated in 2013 from a flooded home in New Jersey, USA, in the aftermath of Hurricane Sandy. Patulin and citrinin gene cluster analyses explained the lack of patulin production in NJ1 compared to R21 and lack of citrinin production in all three strains. A Drosophila bioassay demonstrated that volatiles emitted by P. solitum SA and P. polonicum RS1 were more toxic than those from P. expansum and P. crustosum strains (R27, R11, R21, G10, and R19). The toxicity was hypothesized to be related to production of eight-carbon oxylipins. Putative lipoxygenase genes were identified in P. expansum and P. maximae strains, but not in P. crustosum. Our data will provide a better understanding of Penicillium spp. complex secondary metabolic capabilities, especially concerning the genetic bases of mycotoxins and toxic VOCs.

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Section: Methodsmentioning

confidence: 99%

Genomic Analyses of Penicillium Species Have Revealed Patulin and Citrinin Gene Clusters and Novel Loci Involved in Oxylipin Production

Yin

Pennerman

Jurick

et al. 2021

JoF

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“…For comparison, one P . solitum strain (NJ1) originally obtained as a subculture of a sector of strain 2159A from the Northern Regional Research Laboratory culture collection [ 19 ], and one strain of P. sclerotiorum (113) collected from a flooded home in Manasquan, New Jersey were included [ 20 , 21 ]. Isolates were purified by culturing from single spores, and a preliminary characterization was conducted using macro- and microscopic observations and measurements, including colony color, diameter and texture, conidiospores and conidiophore morphology, and mycotoxin production.…”

Section: Methodsmentioning

confidence: 99%

Characterization of Blue Mold Penicillium Species Isolated from Stored Fruits Using Multiple Highly Conserved Loci

Yin

Zhang

Pennerman

et al. 2017

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Penicillium is a large genus of common molds with over 400 described species; however, identification of individual species is difficult, including for those species that cause postharvest rots. In this study, blue rot fungi from stored apples and pears were isolated from a variety of hosts, locations, and years. Based on morphological and cultural characteristics and partial amplification of the β-tubulin locus, the isolates were provisionally identified as several different species of Penicillium. These isolates were investigated further using a suite of molecular DNA markers and compared to sequences of the ex-type for cognate species in GenBank, and were identified as P. expansum (3 isolates), P. solitum (3 isolates), P. carneum (1 isolate), and P. paneum (1 isolate). Three of the markers we used (ITS, internal transcribed spacer rDNA sequence; benA, β-tubulin; CaM, calmodulin) were suitable for distinguishing most of our isolates from one another at the species level. In contrast, we were unable to amplify RPB2 sequences from four of the isolates. Comparison of our sequences with cognate sequences in GenBank from isolates with the same species names did not always give coherent data, reinforcing earlier studies that have shown large intraspecific variability in many Penicillium species, as well as possible errors in some sequence data deposited in GenBank.

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“…In 2015 to 2017, using colony morphology, microscopic characteristics, and the ITS barcode, we identified two Penicillium species from stored fruits as Penicillium solitum NJ1 and RS1 ( 5 ) and another strain from a flooded home as Penicillium sclerotiorum 113 ( 6 ). At that time, we also analyzed the genome sequences of these three culturable strains ( 7 – 9 ). More recently, upon the publication of a letter to the editor on a series of recommendations to the microbiological community to prevent the taxonomic misidentification of genome-sequenced fungal strains suggested by Houbraken et al ( 10 ), we reperformed BLASTn searches using the Penicillium strains NJ1, RS1, and 113.…”

Section: Replymentioning

confidence: 99%

New Names for Three Penicillium Strains Based on Updated Barcoding and Phylogenetic Analyses

Yin

Jurick

Zhao

et al. 2021

Microbiol Resour Announc

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Genome Sequencing and Analysis of the Filamentous Fungus Penicillium sclerotiorum 113, Isolated after Hurricane Sandy

Cited by 4 publications

References 12 publications

Genomic Analyses of Penicillium Species Have Revealed Patulin and Citrinin Gene Clusters and Novel Loci Involved in Oxylipin Production

Genomic Analyses of Penicillium Species Have Revealed Patulin and Citrinin Gene Clusters and Novel Loci Involved in Oxylipin Production

Characterization of Blue Mold Penicillium Species Isolated from Stored Fruits Using Multiple Highly Conserved Loci

New Names for Three Penicillium Strains Based on Updated Barcoding and Phylogenetic Analyses

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