Genome sequencing and analysis of Talaromyces pinophilus provide insights into biotechnological applications

Li, Chengxi; Zhao, Shuai; Zhang, Ting; Liu, Xian; Liao, Lusheng; Liu, Junliang; Feng, Jia‐Xun

doi:10.1038/s41598-017-00567-0

Cited by 36 publications

(33 citation statements)

References 63 publications

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“…Soil fungi can also produce mycotoxins that contaminate human and animal food and that represent a threat to human and animal health (7). Genomic data on soil fungi have revealed numerous unexplored secondary metabolites, for example, 68 secondary metabolite gene clusters were identified in Talaromyces pinophilus 1-95 alone (8).…”

mentioning

confidence: 99%

Transcription Factor NsdD Regulates the Expression of Genes Involved in Plant Biomass-Degrading Enzymes, Conidiation, and Pigment Biosynthesis in Penicillium oxalicum

Zhao

Wang

et al. 2018

Appl Environ Microbiol

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Soil fungi produce a wide range of chemical compounds and enzymes with potential for applications in medicine and biotechnology. Cellular processes in soil fungi are highly dependent on the regulation under environmentally induced stress, but most of the underlying mechanisms remain unclear. Previous work identified a key GATA-type transcription factor, NsdD (PoxNsdD; also called POX08415), that regulates the expression of cellulase and xylanase genes in PoxNsdD shares 57 to 64% identity with the key activator NsdD, involved in asexual development in In the present study, the regulatory roles of PoxNsdD in were further explored. Comparative transcriptomic profiling revealed that PoxNsdD regulates major genes involved in starch, cellulose, and hemicellulose degradation, as well as conidiation and pigment biosynthesis. Subsequent experiments confirmed that a Δ strain lost 43.9 to 78.8% of starch-digesting enzyme activity when grown on soluble corn starch, and it produced 54.9 to 146.0% more conidia than the Δ parental strain. During cultivation, Δ cultures changed color, from pale orange to brick red, while the Δ cultures remained bluish white. Real-time quantitative reverse transcription-PCR showed that dynamically regulated the expression of a glucoamylase gene (/), an α-amylase gene (/), and a regulatory gene (/), as well as a polyketide synthase gene (//) for yellow pigment biosynthesis and a conidiation-regulated gene (/). Moreover, binding experiments showed that PoxNsdD bound the promoter regions of the above-described genes. This work provides novel insights into the regulatory mechanisms of fungal cellular processes and may assist in genetic engineering of for potential industrial and medical applications. Most filamentous fungi produce a vast number of extracellular enzymes that are used commercially for biorefineries of plant biomass to produce biofuels and value-added chemicals, which might promote the transition to a more environmentally friendly economy. The expression of these extracellular enzyme genes is tightly controlled at the transcriptional level, which limits their yields. Hitherto our understanding of the regulation of expression of plant biomass-degrading enzyme genes in filamentous fungi has been rather limited. In the present study, regulatory roles of a key regulator, PoxNsdD, were further explored in the soil fungus , contributing to the understanding of gene regulation in filamentous fungi and revealing the biotechnological potential of via genetic engineering.

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mentioning

confidence: 99%

Transcription Factor NsdD Regulates the Expression of Genes Involved in Plant Biomass-Degrading Enzymes, Conidiation, and Pigment Biosynthesis in Penicillium oxalicum

Zhao

Wang

et al. 2018

Appl Environ Microbiol

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“…To investigate whether TpRfx1 transcriptionally regulates the expression of amylase genes in T. pinophilus grown on SCS, real-time quantitative reverse transcription PCR (RT-qPCR) was employed, with ∆ TpKu70 as a control under the same cultivation conditions. The transcription levels of all 24 amylase genes in T. pinophilus 1-95 [ 3 ], including five α-amylase genes ( TP03368 , TP03580 , TP04014 , TP07411 , and TP09288 ), five glucoamylase genes ( TP04225 , TP07482 , TP09267 , TP09287, and TP12319 ), 13 α-glucosidase genes ( TP09781 , TP011464 , TP12265 , TP03913 , TP00071 , TP05786 , TP00293 , TP00938 , TP01354 , TP03337 , TP04013 , TP04937 , and TP05120 ), and a gene encoding a 1,4-α-glucan-branching enzyme (TP03955 ) were measured at 48 h after shifting from glucose to SCS. The expression of each tested amylase gene in the ∆ TpKu70 as the control was set to 100%.…”

Section: Resultsmentioning

confidence: 99%

“…Talaromyces pinophilus has been potentially applied in the biotechnological industry due to its ability to produce integrative amylolytic and cellulolytic enzymes [ 3 ]. Amylases, including α-amylase (EC 3.2.1.1), glucoamylase (EC 3.2.1.3), α-glucosidase (EC 3.2.1.20), and 1,4-α-glucan-branching enzyme (EC 2.4.1.18), degrade starches, with α-amylase attacking the α-1,4-glycosidic bonds of amylopectin or amylose to generate varying lengths of straight chains and branched oligosaccharides and glucoamylase breaking α-1,4- or α-1,6-glucosidic linkages at the nonreducing ends of starch chains or dextrin [ 4 ].…”

Section: Introductionmentioning

confidence: 99%

“…Talaromyces pinophilus 1-95 isolated from ploughed soil in China can produce highly active calcium-independent amylase and integrative cellulase [ 2 ], both potentially applicable to plant biomass biorefining. Recently, the T. pinophilus 1-95 genome was sequenced [ 3 ]. In the current study, we screened and identified novel TFs that regulate the expression of amylase and cellulase genes in T. pinophilus through comparative transcriptome profiling and genetic analyses.…”

Section: Introductionmentioning

confidence: 99%

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The transcription factor TpRfx1 is an essential regulator of amylase and cellulase gene expression in Talaromyces pinophilus

Liao

Zhao

Zhang

et al. 2018

Biotechnol Biofuels

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BackgroundPerfect and low cost of fungal amylolytic and cellulolytic enzymes are prerequisite for the industrialization of plant biomass biorefinergy to biofuels. Genetic engineering of fungal strains based on regulatory network of transcriptional factors (TFs) and their targets is an efficient strategy to achieve the above described aim. Talaromyces pinophilus produces integrative amylolytic and cellulolytic enzymes; however, the regulatory mechanism associated with the expression of amylase and cellulase genes in T. pinophilus remains unclear. In this study, we screened for and identified novel TFs regulating amylase and/or cellulase gene expression in T. pinophilus 1-95 through comparative transcriptomic and genetic analyses.ResultsComparative analysis of the transcriptomes from T. pinophilus 1-95 grown on media in the presence and absence of glucose or soluble starch as the sole carbon source screened 33 candidate TF-encoding genes that regulate amylase gene expression. Thirty of the 33 genes were successfully knocked out in the parental strain T. pinophilus ∆TpKu70, with seven of the deletion mutants firstly displaying significant changes in amylase production as compared with the parental strain. Among these, ∆TpRfx1 (TpRfx1: Talaromyces pinophilus Rfx1) showed the most significant decrease (81.5%) in amylase production, as well as a 57.7% reduction in filter paper cellulase production. Real-time quantitative reverse transcription PCR showed that TpRfx1 dynamically regulated the expression of major amylase and cellulase genes during cell growth, and in vitro electrophoretic mobility shift assay revealed that TpRfx1 bound the promoter regions of genes encoding α-amylase (TP04014/Amy13A), glucoamylase (TP09267/Amy15A), cellobiohydrolase (TP09412/cbh1), β-glucosidase (TP05820/bgl1), and endo-β-1,4-glucanase (TP08514/eg1). TpRfx1 protein containing a regulatory factor X (RFX) DNA-binding domain belongs to RFX family.ConclusionWe identified a novel RFX protein TpRFX1 that directly regulates the expression of amylase and cellulase genes in T. pinophilus, which provides new insights into the regulatory mechanism of fungal amylase and cellulase gene expression.Electronic supplementary materialThe online version of this article (10.1186/s13068-018-1276-8) contains supplementary material, which is available to authorized users.

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“…Selain itu, jamur ini mudah tersebar sehingga dalam waktu 3 hari sudah memenuhi petri, yang menyebabkan jamur patogen terhambat pertumbuhannya dan lama kelamaan tertutupi (Gambar 2). Talaromyces pinophilus sebelumnya bernama Penicillium pinophilum (Li et al, 2017). Kemampuan jamur Penicillium dalam menghambat pertumbuhan jamur patogen sudah banyak dilaporkan.…”

Section: Isolat Cpsr7unclassified

Selection of Phylloplane and Endophyte Microbes as Biocontrol for Rubber Leaf Fall Disease (Corynespora cassiicola)

Khaerati¹,

Ferry²,

Rusli³

2018

J. tanam. industri dan penyegar

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Leaf fall disease in rubber caused by Corynespora cassiicola fungi significantly decreases rubber productivity. C. cassiicola causes leaves to fall all year round, a delay in the tapping of immature rubber plants, yield decrease of producing plants, and even death of susceptible clones. The study aimed to obtain phylloplane and endophytic microbes potentially to inhibit the disease, was conducted from January to December 2016. The study used randomized complete design to assess antagonistic fungi and phylloplane and endophytic bacterias toward C. cassiicola in isolates obtained through exploration in West Java and West Kalimantan. Pathogen isolation showed Corynespora sp with pale brown color, single conidia which slightly bended, shaped like a stick that is swollen at the base, with 2–14 septa. Inhibitory analysis found 42 fungi isolates and 19 bacteria isolates potentially inhibiting C. cassiicola. Six fungi isolates have an inhibitory ability of ≥90%, consisting of two phylloplane fungi isolates (DTJF11 and CPSR7) and four endophytic fungi (CEBPM15, CEBPM23, CEBPM27, and CEPR9) with lysis, mycoparasitism, competition, and antibiosis inhibitory mechanism. The identification showed fungi isolate of DTJF11 is classified as Trichoderma asperellum, CPSR7 as Talaromyces pinophilus, and CEBPM15 as Amanita tenuifolia. Potential bacterial isolates as biological agents are BP7, L3, BP3, BP4, BP5 and BP6 isolates, which have inhibitory power of 28.54%–40.94%, with antibiosis inhibition mechanism.

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Genome sequencing and analysis of Talaromyces pinophilus provide insights into biotechnological applications

Cited by 36 publications

References 63 publications

Transcription Factor NsdD Regulates the Expression of Genes Involved in Plant Biomass-Degrading Enzymes, Conidiation, and Pigment Biosynthesis in Penicillium oxalicum

Transcription Factor NsdD Regulates the Expression of Genes Involved in Plant Biomass-Degrading Enzymes, Conidiation, and Pigment Biosynthesis in Penicillium oxalicum

The transcription factor TpRfx1 is an essential regulator of amylase and cellulase gene expression in Talaromyces pinophilus

Selection of Phylloplane and Endophyte Microbes as Biocontrol for Rubber Leaf Fall Disease (Corynespora cassiicola)

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