Genome Sequencing and Analysis of Bacillus pumilus ICVB403 Isolated from Acartia tonsa Copepod Eggs Revealed Surfactin and Bacteriocin Production: Insights on Anti-Staphylococcus Activity

Abstract

Here we show that Bacillus pumilus ICVB403 recently isolated from copepod eggs is able to produce, after 48-72 h of growth in Landy medium, extracellular inhibitory compounds, which are active against Staphylococcus aureus ATCC 25923, methicillin-resistant S. aureus (MRSA) ATCC 43300, MRSA-S1, Staphylococcus epidermidis 11EMB, Staphylococcus warneri 27EMB, and Staphylococcus hominis 13EMB. Moreover, these extracellular inhibitory compound(s) were able to potentiate erythromycin against the aforementioned staphylococci. The minimum inhibitory concentration (MIC) of erythromycin was reduced from 32 μg/mL to 8 μg/mL for MRSA ATCC 43300 and MRSA SA-1 strains, and from 32-64 μg/mL to 4 μg/mL for S. epidermidis 11EMB and S. hominis 13EMB strains.The genome sequencing and analysis of B. pumilus ICVB403 unveiled 3.666.195 nucleotides contained in 22 contigs with a G + C ratio of 42.0%, 3.826 coding sequences, and 73 RNAs. In silico analysis guided identification of two putative genes coding for synthesis of surfactin A, a lipopeptide with 7 amino acids, and for a circular bacteriocin belonging to the circularin A/uberolysin family, respectively.

Genome-guided purification and characterization of polymyxin A1 from Paenibacillus thiaminolyticus SY20: A rarely explored member of polymyxins

“…But it was always insufficient to elucidate the structure. The whole genome information provided us with a new reference for quickly focusing on the gene clusters involved in antimicrobial compounds ( Zidour et al, 2019 ). This was due to the rapid development of diverse bioinformatics tools to mine genomic sequences for the biosynthetic gene clusters of ribosomal and non-ribosomal natural products ( van Heel et al, 2017 ).…”

Antimicrobial Bacillus: Metabolites and Their Mode of Action

“…The following are available online at , Table S1: 47 antimicrobial metabolites from Bacillus . References [ 137 , 138 , 139 , 140 , 141 , 142 , 143 , 144 , 145 , 146 , 147 , 148 , 149 , 150 , 151 , 152 , 153 , 154 , 155 , 156 , 157 , 158 , 159 , 160 , 161 , 162 , 163 , 164 , 165 , 166 , 167 , 168 , 169 , 170 , 171 , 172 , 173 , 174 , 175 , 176 , 177 , 178 , 179 , 180 , 181 , 182 , 183 , 184 , 185 , 186 ,…”

Biossurfactante produzido por linhagem termo-halofílica de >i<Bacillus alveayuensis>/i<: caracterização química e avaliação da atividade tensoativa em meio poroso com óleo e sobre microrganismos

“…Os lipopeptídeos são descritos na literatura como compostos que apresentam grande atividade contra uma ampla gama de bactérias, incluindo patógenos resistentes à meticilina. 19,[222][223][224] A atividade dos lipopeptídeos depende de sua concentração e ocorre pela sua capacidade em desestabilizar as estruturas e funções de membranas biológicas, acarretando no aumento de sua permeabilidade. 225 Os ensaios por disco difusão mostraram uma inibição discreta contra as Os biossurfactantes rompem a membrana celular, causam vazamento de conteúdo celular ou aumentam a pressão osmótica dentro da célula.…”