Genome Sequencing and Analysis of the Hypocrellin-Producing Fungus Shiraia bambusicola S4201

Zhao, Ning; Li, Dan; Guo, Bing-Jing; Tao, Xin; Lin, Xiangmin; Yan, Shi; Chen, Shuanglin

doi:10.3389/fmicb.2020.00643

Cited by 10 publications

(6 citation statements)

References 64 publications

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“…Moreover, the zinc finger transcription factor, which could also regulate the expression of HA biosynthesis genes, was also found to be upregulated. It was believed that the expression levels of genes involved in the HA synthesis pathway were upregulated by a Zn(II)Cys 6 activating transcription factor that was overexpressed in S. bambusicola S4201 [ 56 ]. Among the hub genes in our coexpression network, the highly expressed gene MH01c06g0046321 was annotated as a Zn(II)Cys 6 -type zinc finger protein (Fig.…”

Section: Resultsmentioning

confidence: 99%

Temperature-responsive regulation of the fermentation of hypocrellin A by Shiraia bambusicola (GDMCC 60438)

et al. 2022

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Background Hypocrellin A (HA) is a perylene quinone pigment with high medicinal value that is produced by Shiraia bambusicola Henn. (S. bambusicola) and Hypocrella bambusae (Berk. & Broome) Sacc. (Ascomycetes) with great potential in clinical photodynamic therapy. Submerged cultivation of S. bambusicola is a popular technique for HA production. However, there is not much research on how temperature changes lead to differential yields of HA production. Results The temperature regulation of submerged fermentation is an efficient approach to promote HA productivity. After a 32 °C fermentation, the HA content in the mycelia S. bambusicola (GDMCC 60438) was increased by more than three- and fivefold when compared to that at 28 °C and 26 °C, respectively. RNA sequencing (RNA-seq) analysis showed that the regulation of the expression of transcription factors and genes essential for HA biosynthesis could be induced by high temperature. Among the 496 differentially expressed genes (DEGs) explicitly expressed at 32 °C, the hub genes MH01c06g0046321 and MH01c11g0073001 in the coexpression network may affect HA biosynthesis and cytoarchitecture, respectively. Moreover, five genes, i.e., MH01c01g0006641, MH01c03g0017691, MH01c04g0029531, MH01c04g0030701 and MH01c22g0111101, potentially related to HA synthesis also exhibited significantly higher expression levels. Morphological observation showed that the autolysis inside the mycelial pellets tightly composted intertwined mycelia without apparent holes. Conclusions The obtained results provide an effective strategy in the submerged fermentation of S. bambusicola for improved HA production and reveal an alternative regulatory network responsive to the biosynthesis metabolism of HA in response to environmental signals.

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Section: Resultsmentioning

confidence: 99%

Temperature-responsive regulation of the fermentation of hypocrellin A by Shiraia bambusicola (GDMCC 60438)

et al. 2022

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“…Based on the reported gene clusters for hypocrellin biosynthesis [ 27 – 29 ], we explored the expression changes of DEGs associated with hypocrellin biosynthesis under SNP treatment. De novo sequencing and comparative analysis revealed 113 putative DEGs were enriched in 10 items related to fungal hypocrellin production (Additional file 2 : Table S5 and S6), including ‘polyketide synthase’, ‘hydroxylase’, ‘probable metabolite transport protein’, and so forth.…”

Section: Resultsmentioning

confidence: 99%

“…4 b). The first transcription factor involved in HA biosynthesis is the zinc finger transcription factor zftf [ 29 ]. The zftf gene with a GAL4-like Zn(II)2Cys6 (or C6 zinc) binuclear cluster DNA-binding domain is located in the hypocrellin gene cluster.…”

Section: Discussionmentioning

confidence: 99%

Nitric oxide donor sodium nitroprusside-induced transcriptional changes and hypocrellin biosynthesis of Shiraia sp. S9

Yan

Wang

et al. 2021

Microb Cell Fact

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Background Nitric oxide (NO) is a ubiquitous signaling mediator in various physiological processes. However, there are less reports concerning the effects of NO on fungal secondary metabolites. Hypocrellins are effective anticancer photodynamic therapy (PDT) agents from fungal perylenequinone pigments of Shiraia. NO donor sodium nitroprusside (SNP) was used as a chemical elicitor to promote hypocrellin biosynthesis in Shiraia mycelium cultures. Results SNP application at 0.01–0.20 mM was found to stimulate significantly fungal production of perylenequinones including hypocrellin A (HA) and elsinochrome A (EA). SNP application could not only enhance HA content by 178.96% in mycelia, but also stimulate its efflux to the medium. After 4 days of SNP application at 0.02 mM, the highest total production (110.34 mg/L) of HA was achieved without any growth suppression. SNP released NO in mycelia and acted as a pro-oxidant, thereby up-regulating the gene expression and activity of reactive oxygen species (ROS) generating NADPH oxidase (NOX) and antioxidant enzymes, leading to the increased levels of superoxide anion (O2−) and hydrogen peroxide (H2O2). Gene ontology (GO) analysis revealed that SNP treatment could up-regulate biosynthetic genes for hypocrellins and activate the transporter protein major facilitator superfamily (MFS) for the exudation. Moreover, SNP treatment increased the proportion of total unsaturated fatty acids in the hypha membranes and enhanced membrane permeability. Our results indicated both cellular biosynthesis of HA and its secretion could contribute to HA production induced by SNP. Conclusions The results of this study provide a valuable strategy for large-scale hypocrellin production and can facilitate further understanding and exploration of NO signaling in the biosynthesis of the important fungal metabolites.

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“…In order to detect the production of PQs in the secondary metabolites of S. bambusicola S4201, a linear relationship between the optical density and the concentration of PQs was established 11 . The detection of PQs was carried out by microplate reader (SpectraMax M2).…”

Section: Determination Of Pqs Productionmentioning

confidence: 99%

Nitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide

Zhao

Yue

et al. 2021

Sci Rep

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Shiraia bambusicola has been used as a traditional Chinese medicine for a long history. Its major medicinal active metabolites are perylenequinones, including hypocrellin A, elsinochrome A and so on. At present, the fermentation yield of perylenequinones is low, and its complex biosynthesis and regulatory pathways are still unclear. In this study, nitric oxide, as a downstream signal molecule of hydrogen peroxide, regulates the biosynthesis of perylenequinones. Exogenous addition of 0.01 mM sodium nitroprusside (nitric oxide donor) can promote perylenequinones production by 156% compared with the control. Further research found that hydrogen peroxide and nitric oxide increased the transcriptional level of the biosynthetic genes of hypocrellin A. The results showed that nitric oxide is involved in the biosynthesis and regulation of perylenequinones in Shiraia bambusicola as a signal molecule. In the future, the yield of perylenequinones can be increased by adding exogenous nitric oxide in fermentation.

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Genome Sequencing and Analysis of the Hypocrellin-Producing Fungus Shiraia bambusicola S4201

Cited by 10 publications

References 64 publications

Temperature-responsive regulation of the fermentation of hypocrellin A by Shiraia bambusicola (GDMCC 60438)

Temperature-responsive regulation of the fermentation of hypocrellin A by Shiraia bambusicola (GDMCC 60438)

Nitric oxide donor sodium nitroprusside-induced transcriptional changes and hypocrellin biosynthesis of Shiraia sp. S9

Nitric oxide regulates perylenequinones biosynthesis in Shiraia bambusicola S4201 induced by hydrogen peroxide

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