2020
DOI: 10.1002/jsfa.10952
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Genome sequencing of cold‐adapted Planococcus bacterium isolated from traditional shrimp paste and protease identification

Abstract: BACKGROUND: Psychrophiles have evolved to adapt to freezing environments, and cold-adapted enzymes from these organisms can maintain high catalytic activity at low temperature. The use of cold-adapted enzymes has great potential for the revolution of food and molecular biology industries.RESULTS: In this study, four different strains producing protease were isolated from traditional fermented shrimp paste, one of which, named Planococcus maritimus XJ11 by 16S rRNA nucleotide sequence analysis, exhibited the la… Show more

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Cited by 11 publications
(4 citation statements)
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“…DNA was extracted from samples using the E.Z.N.A.® Fecal DNA Kit (D4015, Omega, Inc., USA) according to the manufacturer's instructions and stored in a −80°C Refrigerator ( 24 ). Samples were sent to LC-Bio (Hang Zhou, China) for PCR amplification.…”
Section: Methodsmentioning
confidence: 99%
“…DNA was extracted from samples using the E.Z.N.A.® Fecal DNA Kit (D4015, Omega, Inc., USA) according to the manufacturer's instructions and stored in a −80°C Refrigerator ( 24 ). Samples were sent to LC-Bio (Hang Zhou, China) for PCR amplification.…”
Section: Methodsmentioning
confidence: 99%
“…From a wide range of annotated Pfam protein domains, only the sequences related the protein and TMAO degradation processes are depicted in Table 6. Similar to the process reported by Leng et al 26 this strategy allowed us to characterise the potential of KM05 to spoil protein‐rich food. Among Pfam annotations assigned to the KM05 genome, 15 annotations were directly involved in proteolytic activity.…”
Section: Resultsmentioning
confidence: 77%
“…Several public annotation databases were used to predict gene functions, obtain related descriptional information and facilitate the search for target functional genes. 39 The detailed results of function annotation of Klebsiella sp. O852 are reported and shown in the Supporting information (Table S3 and Fig.…”
Section: Resultsmentioning
confidence: 99%