Genome sequencing of four strains of Phylotype I, II and IV of Ralstonia solanacearum that cause potato bacterial wilt in India

Patil, Virupaksh U.; Vanishree, G.; Sagar, Vinay; Chauhan, Rajinder Singh; Chakrabarti, S. K.

doi:10.1016/j.bjm.2016.10.016

Cited by 10 publications

(6 citation statements)

References 14 publications

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“…RSSC isolates were frequently reported on potato (n=185), eggplant (n=95), tomato (n=42), pepper (n=33), and ginger (n=17) (Fig 1B). Of the 477 isolates reported in the literature, the phylotype was identified for 245 isolates (Cellier and Prior 2010; Ghorai et al 2022; Ramesh et al 2014; Gurjar et al 2015; Sagar et al 2014; Kumar et al 2014; Patil et al 2017; Cellier et al 2012). In the available data, phylotype I accounts for all of the published reports of RSSC on eggplant, tomato, and pepper in South Asia.…”

Section: Resultsmentioning

confidence: 99%

“…Based on the genome sequences from this study, genomes from other studies (Patil et al 2017(Patil et al , 2020 and prior studies with single gene markers (Ramesh et al 2014), it is clear that there is considerable diversity of phylotype I RSSC in South Asia, consistent with the theory that phylotype I originated in Asia (Villa et al 2005). In addition to the diverse, presumably endemic population of phylotype I isolates, we identified at least two lineages that may have been more recently introduced to Nepal and Bangladesh: SM743/744 and SM732/734, respectively.…”

Section: Discussionmentioning

confidence: 99%

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Characterization ofRalstonia pseudosolanacearumdiversity and screening host resistance to manage bacterial wilt in South Asia

Subedi,

Cowell,

Cope-Arguello

et al. 2023

Preprint

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In South Asia, bacterial wilt pathogens in the Ralstonia solanacearum species complex (RSSC) impose major constraints on eggplant, tomato, and pepper production. To improve the efficacy of bacterial wilt management, the goals of this study were to (1) conduct a survey of RSSC pathogens in Bangladesh and Nepal, (2) characterize the genetic diversity of these isolates, and (3) screen 37 tomato, eggplant, and pepper accessions for resistance to six representative isolates from South Asia. We isolated 99 isolates from Bangladesh and 20 isolates from Nepal and determined that all are phylotype I isolates of the Ralstonia pseudosolanacearum species. We sequenced and assembled draft genomes for 25 isolates. Phylogenomic analyses suggest that there is a wide diversity of endemic phylotype I isolates in South Asia, and possible introductions of two clonal phylotype I lineages into Bangladesh and Nepal. We contextualize our newly described isolates based on prior reports of RSSC diversity in South Asia and global reports of RSSC pathogens on eggplant and pepper. Greenhouse trials revealed multiple tomato, eggplant, and pepper accessions that exhibit promising levels of resistance to six phylotype I isolates from South Asia.

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Section: Resultsmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Characterization ofRalstonia pseudosolanacearumdiversity and screening host resistance to manage bacterial wilt in South Asia

Subedi,

Cowell,

Cope-Arguello

et al. 2023

Preprint

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“…It is persistent. There are no resistant crop varieties available (Patil et al 2017). Additionally, non-target species will be impacted by the increased usage of pesticides (Manjunatha 2016).…”

Section: Introductionmentioning

confidence: 99%

Management of Potato Brown Rot Disease by Chemically and Eco-Friendly Produced CuO-NPs, MgO-NPs

Rabea

Naeem

Balabel

et al. 2023

Preprint

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Background: In terms of production and consumption, the potato crop is one of the major vegetables in Egypt. The brown rot disease causes enormous losses to the potato industry every year. To lower the risk of pesticide residues, this study focuses on the effective suppression of R. solanacearum, the agent responsible for brown rot disease in potatoes, both in vitro and in vivo with efficient, economical, and environmentally friendly medication CuO and MgO metal oxide nanoparticles. Results: In the current study, CuO and MgO metal oxide nanoparticles were produced using a simple chemical process. Using UV-visible spectroscopy, TEM, the Zeta potential, X-ray diffraction (XRD), and Infrared spectroscopy using Fourier transform (FTIR), the average particle size, the morphology, and the structure of nanoparticles were investigated. Antibacterial effects against R. solanacearum's growth were strongly suppressed by CuO-NPs and MgO-NPs at 3 mg/mL; ZOI measured 19.3 mm, and 17 mm, respectively. The (MIC) stands for minimum inhibitory concentration while (MBC) stands for minimum bactericidal concentration for CuO-NPs and MgO-NPs, respectively, they were measured to be 0.5, 0.6, and 0.6, 0.75 mg/mL. As-prepared CuO-NPs and MgO-NPs at MIC concentrations considerably reduced the disease occurrence to 71.2% and 69.4%, respectively, as opposed to 43.0% and 39.5% persisted in the bulk CuSO4 and bulk MgSO4, according to in-vivo application verified by seed dressing with tuber soaking application. As compared to infected plants, the yield, total chlorophyll content, and enzyme efficiency of potatoes all increased significantly. After interactions with CuO-NPs and MgO-NPs, lipid peroxidation and ultrastructural investigations using transmission electron microscopy (TEM) demonstrated that the cytomembrane of bacteria was severely harmed by nanomechanical forces. Conclusion: The promising CuO-NPs and MgO-NPs for use as intelligent substances for agriculture management of the plant pathogen since it paves the way for future investigations on the use of metal oxide nanoparticles as risk-free options to treat plant diseases that are the biggest challenges. Consequently, the notable increase in the yield of the potato crop, photosynthetic pigments, enzymatic activity, and total phenol promote resistance to the Ralstonia solanacearum compared to the infected control.

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“…(FAO). The host of bacterium is more than four hundred plant species and spread across the world (Patil et 2017). This soil born bacterium has been classified into five races and six biovars based on host and trophic traits respectively (He et al, 1983).…”

Section: Introductionmentioning

confidence: 99%

Phylotype Analysis of Ralstonia Solanacearum Causing Bacterial wilt in Eggplants in Orissa in India

Kumar¹,

Ramachandran²,

Yadav³

2019

IJTSRD

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Eggplant is prone to attack by several pests including bacteria, fungi, nematodes and insects. In this study, we have analyzed phylotype of bacterial wilt (Ralstonia solanacearum) infection in eggplant plants collected from Bhubaneswar (Orissa) in India. Bacterial wilt symptomatic five plant samples were collected from brinjal field in Bhubaneswar in 2016. The samples were macerated in sterile distilled water and grown on Kelman's triphenyltetrazolium chloride (TZC) agar media. Total genomic DNA of the bacterium were extracted and subjected to PCR amplification using the R. solanacearum specific universal primer pair 759/760. An expected single 280 bp fragment amplified in all the samples confirmed the identity of these as Ralstonia. To reconfirmed isolate of bacterium, the amplicon was sequenced in sequencer. In NCBI blast, the nucleotide sequence was 100% similar with Ralstonia solanacearum strain RS-lpxC-DOB-1 (AB910593) and the sequence was submitted in NCBI database under Acc. No. KY393266. To determined phylotype of strain used specific multiplex PCR with phylotype specific primers (Nmult: 21F1/2, Nmult: 22InF, Nmult: 23AF, Nmult: 22RR) revealed that all the five infected samples belonged to phylotype I as a 144-bp amplicon were observed in agarose gel. On the basis of above finding concluded that the bacterial wilt infected eggplant collected from Bhubaneswar was Ralostonia solanacearum, Phylotype I.

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Genome sequencing of four strains of Phylotype I, II and IV of Ralstonia solanacearum that cause potato bacterial wilt in India

Cited by 10 publications

References 14 publications

Characterization ofRalstonia pseudosolanacearumdiversity and screening host resistance to manage bacterial wilt in South Asia

Characterization ofRalstonia pseudosolanacearumdiversity and screening host resistance to manage bacterial wilt in South Asia

Management of Potato Brown Rot Disease by Chemically and Eco-Friendly Produced CuO-NPs, MgO-NPs

Phylotype Analysis of Ralstonia Solanacearum Causing Bacterial wilt in Eggplants in Orissa in India

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