Genome shuffling: a method to improve biotechnological processes

Leja, Katarzyna; Myszka, Kamila; Czaczyk, Katarzyna

doi:10.5114/bta.2011.46551

Cited by 18 publications

(13 citation statements)

References 37 publications

Supporting

Mentioning

Contrasting

Unclassified

Order By: Relevance

“…Improvement of valuable metabolites from Chlorella and Dunaliella microalgae was done using biotechnological methods employing somatic hybridization by protoplast fusion [8]. This intergeneric fusion enables nuclear and cytoplasmic genomes to be combined, fully or partially, at the intergeneric levels to circumvent naturally occurring sexual incompatibility barriers [9] [10]. Fusant microalgae has gained considerable importance in combining valuable product on both microalgae.…”

Section: Introductionmentioning

confidence: 99%

Detection of Bacteria and Fungi Associated with Penaeus Monodon Postlarvae Mortality

Kusumaningrum

Zainuri

2015

Procedia Environmental Sciences

View full text Add to dashboard Cite

Diseases on shrimp postlarvae caused by bacterial and fungal still remain an important concern due to their economic values. Application of microalgae as natural feed has brought promising results for growth of shrimp postlarvae. The aim of this research is to apply fusant of Dunaliella and Chlorella microalgae and to detect feed effect on microbial diseases resistance on postlarvae. The research was conducted using bacterial and fungi from contaminated shrimp postlarvae. Microbial species were characterized based on morphological and biochemical tests. Total number of bacteria ranging from 21.7 10 5 cfu to 32 10 5 cfu while fungal calculation about 6 10 2 cfu. Microorganisms analysis presumably belong to genus Vibrio, Pseudomonas, Aeromonas, Alcaligenes, Bacillus, Staphylococcus, Hafnia and Fusarium. The absence of V. harveyi pathogen indicated that the fusant serve as the main source on increasing of resistance to diseases and thus reducing the mortality of shrimp postlarvae.

show abstract

Section: Introductionmentioning

confidence: 99%

Detection of Bacteria and Fungi Associated with Penaeus Monodon Postlarvae Mortality

Kusumaningrum

Zainuri

2015

Procedia Environmental Sciences

View full text Add to dashboard Cite

show abstract

“…In order to improve the performances of solventogenic Clostridia, genetic approaches, such as mutagenesis and metabolic engineering, have been followed (Gong et al 2009 ; Leja et al 2011 ; Patnaik 2008 ). Metabolic engineering approaches have been mostly targeted toward improved product yields (Lütke-Eversloh 2014 ), in most cases butanol (Schiel-Bengelsdorf et al 2013 ).…”

Section: Introductionmentioning

confidence: 99%

Improving isopropanol tolerance and production of Clostridium beijerinckii DSM 6423 by random mutagenesis and genome shuffling

Gérando

Fayolle-Guichard

Rudant

et al. 2016

Appl Microbiol Biotechnol

View full text Add to dashboard Cite

Random mutagenesis and genome shuffling was applied to improve solvent tolerance and isopropanol/butanol/ethanol (IBE) production in the strictly anaerobic bacteria Clostridium beijerinckii DSM 6423. Following chemical mutagenesis with N-methyl-N-nitro-N-nitrosoguanidine (NTG), screening of putatively improved strains was done by submitting the mutants to toxic levels of inhibitory chemicals or by screening for their tolerance to isopropanol (>35 g/L). Suicide substrates, such as ethyl or methyl bromobutyrate or alcohol dehydrogenase inhibitors like allyl alcohol, were tested and, finally, 36 mutants were isolated. The fermentation profiles of these NTG mutant strains were characterized, and the best performing mutants were used for consecutive rounds of genome shuffling. Screening of strains with further enhancement in isopropanol tolerance at each recursive shuffling step was then used to spot additionally improved strains. Three highly tolerant strains were finally isolated and able to withstand up to 50 g/L isopropanol on plates. Even if increased tolerance to the desired end product was not always accompanied by higher production capabilities, some shuffled strains showed increased solvent titers compared to the parental strains and the original C. beijerinckii DSM 6423. This study confirms the efficiency of genome shuffling to generate improved strains toward a desired phenotype such as alcohol tolerance. This tool also offers the possibility of obtaining improved strains of Clostridium species for which targeted genetic engineering approaches have not been described yet.

show abstract

“…Protoplast suspension was equally divided into two parts. One part was irradiated with UV for 30 min, and the other part was treated with heat at 60 • C for 2 h [70]. Inactivated protoplasts of the two different methods were pooled after centrifugation and resuspended in a HEPES buffer.…”

Section: Genome Shufflingmentioning

confidence: 99%

Comparative Analysis of Structural Variations Due to Genome Shuffling of Bacillus Subtilis VS15 for Improved Cellulase Production

Lakshmi

Drendel

Petrovski

et al. 2020

IJMS

View full text Add to dashboard Cite

Cellulose is one of the most abundant and renewable biomass products used for the production of bioethanol. Cellulose can be efficiently hydrolyzed by Bacillus subtilis VS15, a strain isolate obtained from decomposing logs. A genome shuffling approach was implemented to improve the cellulase activity of Bacillus subtilis VS15. Mutant strains were created using ethyl methyl sulfonate (EMS), N-Methyl-N′ nitro-N-nitrosoguanidine (NTG), and ultraviolet light (UV) followed by recursive protoplast fusion. After two rounds of shuffling, the mutants Gb2, Gc8, and Gd7 were produced that had an increase in cellulase activity of 128%, 148%, and 167%, respectively, in comparison to the wild type VS15. The genetic diversity of the shuffled strain Gd7 and wild type VS15 was compared at whole genome level. Genomic-level comparisons identified a set of eight genes, consisting of cellulase and regulatory genes, of interest for further analyses. Various genes were identified with insertions and deletions that may be involved in improved celluase production in Gd7. Strain Gd7 maintained the capability of hydrolyzing wheatbran to glucose and converting glucose to ethanol by fermentation with Saccharomyces cerevisiae of the wild type VS17. This ability was further confirmed by the acidified potassium dichromate (K2Cr2O7) method.

show abstract

Genome shuffling: a method to improve biotechnological processes

Cited by 18 publications

References 37 publications

Detection of Bacteria and Fungi Associated with Penaeus Monodon Postlarvae Mortality

Detection of Bacteria and Fungi Associated with Penaeus Monodon Postlarvae Mortality

Improving isopropanol tolerance and production of Clostridium beijerinckii DSM 6423 by random mutagenesis and genome shuffling

Comparative Analysis of Structural Variations Due to Genome Shuffling of Bacillus Subtilis VS15 for Improved Cellulase Production

Contact Info

Product

Resources

About