2013
DOI: 10.1007/s12010-013-0297-9
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Genome Shuffling of Penicillium citrinum for Enhanced Production of Nuclease P1

Abstract: Genome shuffling is a powerful approach for efficiently engineering industrial microbial strains with interested phenotypes. Here we reported a high producer of nuclease P1, Penicillium citrinum G-16, that was bred by the classical physics-mutagenesis and genome shuffling process. The starting populations were generated by (60)Co γ-irradiation mutagenesis. The derived two protoplast fractions were inactivated by heat-treatment and ultraviolet radiation respectively, then mixed together and subjected to recursi… Show more

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Cited by 7 publications
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