2008
DOI: 10.1007/s10295-008-0481-z
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Genome shuffling to improve thermotolerance, ethanol tolerance and ethanol productivity of Saccharomyces cerevisiae

Abstract: Genome shuffling is a powerful strategy for rapid engineering of microbial strains for desirable industrial phenotypes. Here we improved the thermotolerance and ethanol tolerance of an industrial yeast strain SM-3 by genome shuffling while simultaneously enhancing the ethanol productivity. The starting population was generated by protoplast ultraviolet irradiation and then subjected for the recursive protoplast fusion. The positive colonies from the library, created by fusing the inactivated protoplasts were s… Show more

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Cited by 173 publications
(103 citation statements)
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“…The most common approach to altering the thermal growth parameters of microbes is genetic engineering, and a panoply of molecular biological tools have been brought to bear on the problem (1,8,16,22,34,38,55,62). The most success has been achieved using advanced high-throughput recombinant engineering techniques, because they either sample many random genetic variations (some of which may have originated in thermophiles) or affect highly pleiotropic genes and thus can access the genetic or biochemical diversity required to significantly alter complex traits, such as optimal growth temperature (T opt ) or maximal growth temperature (T max ).…”
mentioning
confidence: 99%
“…The most common approach to altering the thermal growth parameters of microbes is genetic engineering, and a panoply of molecular biological tools have been brought to bear on the problem (1,8,16,22,34,38,55,62). The most success has been achieved using advanced high-throughput recombinant engineering techniques, because they either sample many random genetic variations (some of which may have originated in thermophiles) or affect highly pleiotropic genes and thus can access the genetic or biochemical diversity required to significantly alter complex traits, such as optimal growth temperature (T opt ) or maximal growth temperature (T max ).…”
mentioning
confidence: 99%
“…It is a desirable approach to engineering SSL tolerance, because the complexity of the genetic factors possibly involved is difficult to hypothesize or to rationally engineer. This technology has been used successfully to shuffle the genomes of bacteria and eukaryotes for other specific traits, such as improved antibiotic production (42), tolerance and degradation of pentachlorophenol (8), and tolerance of low pHs (29,40), high temperatures, or ethanol (14,34).…”
mentioning
confidence: 99%
“…However, due to the increasing demand of producing larger and cheaper ethanol volumes worldwide, S. cerevisiae is further challenged with new process requirements. Specifically, yeasts with higher thermotolerance are needed to fulfill fermentation at temperature above 40°C which will largely reduce cooling costs and help preventing contamination (2,3). High-temperature cultivation will also benefit a simultaneous saccharification and fermentation process, given that the current compromise between the optimal fermentation temperature (30 -35°C) and saccharification temperature (Ͼ50°C) considerably limits the rate and efficiency of enzymatic hydrolysis (2,4).…”
mentioning
confidence: 99%