Genome size of the European domestic goose (Anser anser domesticus)

Abstract

2009. Genome size of the European domestic goose (Anser anser domesticus). Can. J. Anim. Sci. 89: 449Á455. This work is aimed at determining the C-DNA contained within the nuclei of different types of cells in the domestic goose Anser anser. Cells from the lungs, skin, pancreas, kidney, spleen, liver, heart, brain, blood, ovary and testicle were analysed. Cells from the blood, ovary and testicle were smeared onto microscopic glasses, whereas slides from the other organs and tissues were prepared using the paraffin technique. DNA content, as visualized by the Feulgen reaction using computerized image analysis, was examined in9200 nuclei of every type of cell. Chicken erythrocytes were used as reference material. Different concentrations of chromatin within cell nuclei were observed, from small, dispersed clods to an entirely filled nucleus surface. It was stated that the average C-DNA content in the domestic European goose amounts to 1.30690.327 pg, which gives goose DNA a length in base pair of 1.277 )10 9 9 0.320 )10 9 bp after adjustment. The correlation between nucleus size and the C-DNA content was positive and high. In all cell types it exceeded 0.6. The highest was observed in lung and ovary cells, the lowest in skin and the pancreas. The majority of all cells (57.34%) contain DNA at the range between 1.0 to 1.5 pg, especially those from erythrocytes and the pancreas (82 and 76% respectively). Liver cells demonstrate a tendency toward an amount that is higher than 1.5% of the DNA (78.61% cells). Heart cells reveal a tendency downward (98.99% below 1.5 pg.). Less than 1.0 pg of DNA was observed in 17.13% of all examined cells.Key words: Domestic goose, cell, cell nuclei, Feulgen reaction, genome size, DNA mass Andraszek, K., Wo´jcik, E., Gruz : ewska, A. et Smalec, E. 2009. Taille du ge´nome de l'oie cendre´e d'Europe (Anser anser domesticus). Can. J. Anim. Sci. 89: 449Á455. Ce travail devait pre´ciser la nature de l'ADN-C pre´sent dans le noyau de diverses cellules de l'oie domestique (Anser anser). Les auteurs ont analyse´les cellules des poumons, de l'e´piderme, du pancre´as, des reins, de la rate, du foie, du caeur, du cerveau, du sang, des ovaires et des testicules. Celle du sang, des ovaires et des testicules ont e´te´examine´es par frottis sur des lames de microscope, alors que celles des autres organes et tissus ont e´te´pre´pare´es avec de la paraffine. La concentration d'ADN a e´te´e´tablie par la re´action de Feulgen suivie d'analyse de l'image sur ordinateur pour9200 noyaux de chaque type de cellule. Les e´rythrocytes de poulet ont servi de point de re´fe´rence. La concentration de chromatine varie dans le noyau, allant de petits amas disperse´s a`un noyau entie`rement plein en surface. On estime que la teneur moyenne en ADN-C se situe a`1,30690,327 pg chez l'oie, ce qui donne 1,277 )10 9 9 0,320 )10 9 paires de bases apre`s correction. La taille du noyau et la concentration d'ADN-C pre´sentent une forte corre´lation positive. Cette corre´lation de´passe 0,6 dans toutes les cellules. La plus for...

Immunocytological Analysis of Meiotic Recombination in the Gray Goose (<b><i>Anser anser</i></b>)

“…Figure 1 shows surface spreads of an oocyte and a spermatocyte of the gray goose after immunolocalization of SYCP3, MLH1, and centromere proteins. Ovaries of goslings on day 4 after hatching and testes of a 1-year-old male gave SC spreads more suitable for analysis than previous attempts employing gonads of 1-day-old females and 17-year-old ganders [Andraszek et al, 2009;Andraszek and Smalec, 2012].…”

“…That was equal to 3,682 cM for female meiosis and 2,947 cM for male meiosis. By dividing the genetic map length by the genome size of A. anser (1,277 Mb [Andraszek et al, 2009]), we estimated the RR as 2.9 cM/Mb in female meiosis and 2.3 cM/Mb in male meiosis ( Table 2 ).…”