Genome Size Variation and Plant Systematics

Ohri, Deepak

doi:10.1006/anbo.1998.0765

Cited by 162 publications

(115 citation statements)

References 107 publications

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“…Although some works confirm the existence of noticeable intraspecific variability in the content of nuclear DNA (Ohri, 1998), other authors consider nuclear DNA content as a species-specific character (Greilhuber, 1998). Our data on nuclear DNA content in L. serriola, with such limited variation among 10 samples, tend to support the view of Greilhuber (1998).…”

Section: Dna Content Variation In L Serriolasupporting

confidence: 66%

North American Continent - A New Source of Wild Lactuca Spp. Germplasm Variability for Future Lettuce Breeding

Lebeda¹,

Doležalová²,

Kitner³

et al. 2011

Acta Hortic.

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Section: Dna Content Variation In L Serriolasupporting

confidence: 66%

North American Continent - A New Source of Wild Lactuca Spp. Germplasm Variability for Future Lettuce Breeding

Lebeda¹,

Doležalová²,

Kitner³

et al. 2011

Acta Hortic.

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“…In addition to cytogenetic analysis, which is a powerful tool for the identification of variations in chromosome number and structure, the study of nuclear DNA amounts by flow cytometry brings important information about genomic variations (Dolezel et al 2007). According to Ohri (1998), there is a remarkable difference in genome size at the infrageneric level, which can be used to demarcate various taxa. Moreover, DNA markers, such as random amplified polymorphic DNA (RAPD), are also useful tools for understanding the relationships among different accessions and genotypes (Saxena andChandra 2006, Sheidai et al 2007).…”

Section: Introductionmentioning

confidence: 99%

Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)

Pierre

Sousa

Davide

et al. 2011

An. Acad. Bras. Ciênc.

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Cytogenetic analyses, of pollen viability, nuclear DNA content and RAPD markers were employed to study three chemotypes of Lippia alba (Mill.) (Verbenaceae) in order to understand the genetic variation among them. Different ploidy levels and mixoploid individuals were observed. This work comprises the first report of different chromosome numbers (cytotypes) in L. alba. The chromosome numbers of La2-carvone and La3-linalool chemotypes suggested that they are polyploids. Flow cytometric analysis showed an increase of nuclear DNA content that was not directly proportional to ploidy level variation. A cluster analysis based on RAPD markers revealed that La3-linalool shares genetic markers with La1-citral and La2-carvone. The analysis showed that the majority of genetic variation of La3-linalool could be a consequence of mixoploidy. Our data indicates that sexual reproduction among those three chemotypes is unlikely and suggests the beginning of reproductive isolation. The results demonstrated that chromosome analysis, nuclear DNA content estimation and RAPD markers constitute excellent tools for detecting genetic variation among L. alba chemotypes.

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“…Where many species in a genus have the same chromosome numbers, differences in nuclear DNA content are often present and have proven to be very effective in delimiting infrageneric divisions in a number of taxa (Ohri 1998). Genome size has been demonstrated to often vary a factor 1.5-3 between taxa with identical chromosome numbers (Ohri 1998;Yokoya et al 2000;Zonneveld 2001;Zonneveld and van Iren 2001;Ellul et al 2002;Zonneveld et al 2003a, b;Hirsch et al 2004: Albach andGreilhuber 2004;Zonneveld and Duncan 2006). Moreover, Greilhuber (1998Greilhuber ( , 2005 has clearly shown that intraspecific variation of genome size is much less than assumed.…”

Section: Introductionmentioning

confidence: 99%

“…Major decreases in genome size occur less frequently and such decreases have been observed especially following a doubling of the total genome by polyploidization (Ohri 1998), or the change from perennial to annual habit (Bennett 1972).…”

Section: Introductionmentioning

confidence: 99%

The systematic value of nuclear DNA content for all species of Narcissus L. (Amaryllidaceae)

Zonneveld

2008

Plant Syst Evol

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The taxonomy of all species of Narcissus (Amaryllidaceae), an important horticultural crop, has not been investigated recently. As a new approach, genome size was determined by flow cytometry with propidium iodide from 375 accessions. The somatic nuclear DNA contents (2C) were shown to range from 14 to 38 pg for the diploids. Narcissus assoanus and N. gaditanus are, based on their nuclear DNA content, removed from section Apodanthi and placed in a new section Juncifolii. The different ploidy levels and species involved were entangled for N. ''fernandesii'' s.l. and a new allotetraploid form is named here. Section Pseudonarcissus was much more heterogeneous in nuclear DNA content than expected. Sixty-five accessions of N. pseudonarcissus possessed, with 23.7 pg, similar amounts of DNA. However, several species from this section were clearly distinctive in nuclear DNA content. It runs from the diploid N. primigenius with 21.7 pg to the also diploid N. nevadensis with 38.2 pg. Also N. abscissus and N. moleroi are with about 26 pg clearly different from N. pseudonarcissus. For the first time, in 11 accessions, hexaploidy was found in N. pseudonarcissus ssp. bicolor. A new section Nevadensis with 30-39 pg of nuclear DNA was split off from the section Pseudonarcissus with now 21-27 pg. A nonoploid N. dubius with 96.3 pg has by far the highest amount of nuclear DNA and can be calculated to have the highest ploidy ever reported in Narcisssus. The total number of Narcissus species was determined as 36, nine more than in Flora Europaea and they were divided up in two subgenera and 11 sections. Flow cytometry is shown to produce easily obtainable and original systematic data that lead to new insights. Genome size or C-value turns out to be one of the most salient features to define the status of the species in the genus Narcissus.

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Genome Size Variation and Plant Systematics

Cited by 162 publications

References 107 publications

North American Continent - A New Source of Wild Lactuca Spp. Germplasm Variability for Future Lettuce Breeding

North American Continent - A New Source of Wild Lactuca Spp. Germplasm Variability for Future Lettuce Breeding

Karyotype analysis, DNA content and molecular screening in Lippia alba (Verbenaceae)

The systematic value of nuclear DNA content for all species of Narcissus L. (Amaryllidaceae)

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