Genome sizes of four important medicinal species in Kadsura by flow cytometry

Xu, Jing; Wei, Xueping; Liu, Jiushi; Qi, Yanfeng; Zhang, Bengang; Liu, Haitao; Xiao, Pei‐Gen

doi:10.1016/j.chmed.2021.05.002

Cited by 3 publications

(2 citation statements)

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“…The qualified libraries were sequenced by the Illumina Hiseq platform to generate 150 bp paired-end reads ( Miller et al, 2007 ; Baird et al, 2008 ). At present, there is no complete genome of the Kadsura species, so the K. interior genome size estimated by flow cytometry (FCM) is used as a reference for sequencing the depth ( Xu et al, 2021 ).…”

Section: Methodsmentioning

confidence: 99%

“…As the whole genome of Kadsura has not yet been sequenced, and the genome of the is around 7.36 G, which makes it difficult to conduct research through the whole genome ( Xu et al, 2021 ). RAD-seq, a high-throughput sequencing technology, enables the development of large-scale SNPs without relying on the reference genomes to provide greater phylogenetic resolution than conventional gene regions ( Miller et al, 2007 ).…”

Section: Introductionmentioning

confidence: 99%

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RAD-Seq and Ecological Niche Reveal Genetic Diversity, Phylogeny, and Geographic Distribution of Kadsura interior and Its Closely Related Species

Dong

Wei²,

Qiang³

et al. 2022

Front. Plant Sci.

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Most plants of Kadsura have economic value and medicinal application. Among them, K. interior and its closely related species have been demonstrated to have definite efficacy. However, the taxonomy and phylogenetic relationship of Kadsura in terms of morphology and commonly used gene regions remain controversial, which adversely affects its rational application. In this study, a total of 107 individuals of K. interior, K. heteroclita, K. longipedunculata, K. oblongifolia, and K. coccinea were studied from the perspectives of genetic diversity, phylogeny, and ecology via single nucleotide polymorphisms (SNPs) developed through restriction site-associated DNA sequencing (RAD-seq). Based on these SNPs, the genetic diversity, phylogenetic reconstruction, and population genetic structure were analyzed. Subsequently, divergence time estimation and differentiation scenario simulation were performed. Meanwhile, according to the species distribution records and bioclimatic variables, the Last Glacial Maximum and current potential distributions of five species were constructed, and the main ecological factors affecting the distribution of different species were extracted. The FST calculated showed that there was a moderate degree of differentiation among K. heteroclita, K. longipedunculata, and K. oblongifolia, and there was a high degree of genetic differentiation between K. interior and the above species. The phylogenetic tree indicated that each of the species was monophyletic. The results of population genetic structure and divergence scenario simulation and D-statistics showed that there were admixture and gene flow among K. heteroclita, K. longipedunculata, and K. oblongifolia. The results of ecological niche modeling indicated that the distribution areas and the bioclimatic variables affecting the distribution of K. interior and its related species were different. This study explored the differences in the genetic divergence and geographical distribution patterns of K. interior and its related species, clarifying the uniqueness of K. interior compared to its relatives and providing a reference for their rational application in the future.

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Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%