Genome Structure, Mapping and Expression of the Tropomyosin Genetmy-1ofCaenorhabditis elegans

Kagawa, Hiroaki; Sugimoto, Koken; Matsumoto, Hiroshi; Inoue, Tomio; Imadzu, Hirofumi; Takuwa, Kyodo; Sakube, Yasuji

doi:10.1006/jmbi.1995.0459

Cited by 51 publications

(80 citation statements)

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“…Alternatively, an uncharacterized CeTM isoform(s) might be expressed in the oocytes. The CeTM gene, tmy-1/lev-11, undergoes alternative splicing to produce multiple isoforms (Kagawa et al, 1995;Anyanful et al, 2001). However, a nonmuscle isoform of CeTM has not been identified.…”

Section: Discussionmentioning

confidence: 99%

Tropomyosin and Troponin Are Required for Ovarian Contraction in theCaenorhabditis elegansReproductive System

Ono

2004

MBoC

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Ovulation in the nematode Caenorhabditis elegans is coordinated by interactions between the somatic gonad and germ cells. Myoepithelial sheath cells of the proximal ovary are smooth muscle-like cells, but the regulatory mechanism of their contraction is unknown. We show that contraction of the ovarian muscle requires tropomyosin and troponin, which are generally major actin-linked regulators of contraction of striated muscle. RNA interference of tropomyosin or troponin C caused sterility by inhibiting ovarian contraction that is required for expelling mature oocytes into the spermatheca where fertilization takes place, thus causing accumulation of endomitotic oocytes in the ovary. Tropomyosin and troponin C were associated with actin filaments in the myoepithelial sheath, and the association of troponin C with actin was dependent on tropomyosin. A mutation in the actin depolymerizing factor/cofilin gene suppressed the ovulation defects by RNA interference of tropomyosin or troponin C. These results strongly suggest that tropomyosin and troponin are the actin-linked regulators for contraction of ovarian muscle in the C. elegans reproductive system.

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Section: Discussionmentioning

confidence: 99%

Tropomyosin and Troponin Are Required for Ovarian Contraction in theCaenorhabditis elegansReproductive System

Ono

2004

MBoC

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“…SDS-polyacrylamide gel electrophoresis (SDS-PAGE) and protein transfer to nitrocellulose filter were performed as described before (Laemmli, 1970;Towbin et al, 1979;Kagawa et al, 1995). Samples were prepared as follows; worms were transferred into 2 µl of phosphate buffer (20 mM), and equal volume of 2X Laemmli buffer was added.…”

Section: Western Analysismentioning

confidence: 99%

“…anti-MHC C monoclonal antibody, 9.2.1 (Miller et al, 1986); anti-slime mold actin polyclonal antibody (kind gift from K. Owaribe, Nagoya University); anti-tropomyosin antibody (Kagawa et al, 1995;Anyanful et al, 2001). As the secondary antibodies, horseradish peroxidase-conjugated donkey anti-rabbit IgG (Amersham Bioscience, Piscataway, NJ, USA) and horseradish peroxidase-conjugated sheep anti-mouse IgG (Amersham Bioscience, Piscataway, NJ, USA) were used.…”

Section: Western Analysismentioning

confidence: 99%

“…animals, which have a Glu342 to Lys substitution, form abnormal thick filaments but produce normal levels of paramyosin protein the same size as that of wild-type (Waterston et al, 1977;Gengyo-Ando and Kagawa, 1991). The tropomyosin two types of the four isoforms, TM I/II and TM III/IV are expressed in body-wall, pharyngeal and gut muscles, respectively (Kagawa et al, 1995;Anyanful et al, 2001).…”

Section: Introductionmentioning

confidence: 99%

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Spaceflight results in increase of thick filament but not thin filament proteins in the paramyosin mutant of Caenorhabditis elegans

Adachi

Takaya

Kuriyama

et al. 2008

Advances in Space Research

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We have investigated the effect of microgravity during spaceflight on body-wall muscle fiber size and muscle proteins in the paramyosin mutant of Caenorhabditis elegans.Both mutant and wild-type strains were subjected to 10 days of microgravity during spaceflight and compared to ground control groups. No significant change in muscle fiber size or quantity of the protein was observed in wild-type worms; where as atrophy of body-wall muscle and an increase in thick filament proteins were observed in the paramyosin mutant unc-15(e73) animals after spaceflight. We conclude that the mutant with abnormal muscle responded to microgravity by increasing the total amount of muscle protein in order to compensate for the loss of muscle function.

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“…body wall muscles produced under the control of distal and internal promoters and splicing choice, respectively (Kagawa et al, 1995, Anyanful et al, 2001). The lev-11(st557) mutant animal, which was isolated as a Pat animal (Williams and Waterston 1994) produces no tropomyosin due to a mutation at the splice donor site of exon 1 (Kagawa et al, 1997).…”

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confidence: 99%

C. Elegans Model for Studying Tropomyosin and Troponin Regulations of Muscle Contraction and Animal Behavior

Kagawa

Takaya

Ruksana

et al.

Regulatory Mechanisms of Striated Muscle Contraction

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Genome Structure, Mapping and Expression of the Tropomyosin Genetmy-1ofCaenorhabditis elegans

Cited by 51 publications

References 0 publications

Tropomyosin and Troponin Are Required for Ovarian Contraction in theCaenorhabditis elegansReproductive System

Tropomyosin and Troponin Are Required for Ovarian Contraction in theCaenorhabditis elegansReproductive System

Spaceflight results in increase of thick filament but not thin filament proteins in the paramyosin mutant of Caenorhabditis elegans

C. Elegans Model for Studying Tropomyosin and Troponin Regulations of Muscle Contraction and Animal Behavior

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