Genome structure of mycobacteriophage D29: implications for phage evolution 1 1Edited by J. Karn

Ford, Michael E.; Sarkis, Gary J.; Belanger, Aimee E.; Hendrix, Roger W.; Hatfull, Graham F.

doi:10.1006/jmbi.1997.1610

Cited by 178 publications

(164 citation statements)

References 34 publications

Supporting

Mentioning

153

Contrasting

Unclassified

Order By: Relevance

“…The terminase gene, transporting DNA into the proheads prior to attachment of the tail (54)(55)(56), is located close to the structural gene operon and far from the physical end of the genome, more than 20 kbp. This is also observed in the genomes of cluster A myobacteriophages (13,30,(56)(57)(58).…”

Section: Discussionsupporting

confidence: 54%

The First Structure of a Mycobacteriophage, the Mycobacterium abscessus subsp. bolletii Phage Araucaria

Sassi

Bebeacua

Drancourt

et al. 2013

J Virol

View full text Add to dashboard Cite

The unique characteristics of the waxy mycobacterial cell wall raise questions about specific structural features of their bacteriophages. No structure of any mycobacteriophage is available, although ϳ3,500 have been described to date. To fill this gap, we embarked in a genomic and structural study of a bacteriophage from Mycobacterium abscessus subsp. bolletii, a member of the Mycobacterium abscessus group. This opportunistic pathogen is responsible for respiratory tract infections in patients with lung disorders, particularly cystic fibrosis. M. abscessus subsp. bolletii was isolated from respiratory tract specimens, and bacteriophages were observed in the cultures. We report here the genome annotation and characterization of the M. abscessus subsp. bolletii prophage Araucaria, as well as the first single-particle electron microscopy reconstruction of the whole virion. Araucaria belongs to Siphoviridae and possesses a 64-kb genome containing 89 open reading frames (ORFs), among which 27 could be annotated with certainty. Although its capsid and connector share close similarity with those of several phages from Gram-negative (Gram ؊ ) or Gram ؉ bacteria, its most distinctive characteristic is the helical tail decorated by radial spikes, possibly host adhesion devices, according to which the phage name was chosen. Its host adsorption device, at the tail tip, assembles features observed in phages binding to protein receptors, such as phage SPP1. All together, these results suggest that Araucaria may infect its mycobacterial host using a mechanism involving adhesion to cell wall saccharides and protein, a feature that remains to be further explored.

show abstract

Section: Discussionsupporting

confidence: 54%

The First Structure of a Mycobacteriophage, the Mycobacterium abscessus subsp. bolletii Phage Araucaria

Sassi

Bebeacua

Drancourt

et al. 2013

J Virol

View full text Add to dashboard Cite

show abstract

“…To confirm the predicted functions of these regions of the wEf11 genome, and to develop a potentially useful agent for phage therapy, we wished to determine whether deletion or replacement of these sites in phage wEf11 would result in a derivative virus with virulent rather than temperate properties. There is substantial precedent for temperate to virulent conversion of phage by modification of these genomic sites (Bailone & Devoret, 1978;Flashman, 1978;Donnelly-Wu et al, 1993;Bruttin & Brüssow, 1996;Ford et al, 1998).…”

Section: Discussionmentioning

confidence: 99%

Genetic modifications to temperate Enterococcus faecalis phage ϕEf11 that abolish the establishment of lysogeny and sensitivity to repressor, and increase host range and productivity of lytic infection

et al. 2013

View full text Add to dashboard Cite

“…Table 2 lists the oligos along with their sequences. The mycobacteriophage D29 was a kind gift from Graham Hatfull (University of Pittsburgh) and was cultured following the published protocol (22). The genomic DNA of the phage was isolated as described (27).…”

Section: Methodsmentioning

confidence: 99%

“…Two of these genes encode Lysin A and Lysin B that sandwich one other gene coding for a membrane pore-forming protein, holin (20). The Lysin A (coded by gp10) is responsible for the hydrolysis of PG, 3 whereas Lysin B (coded by gp12) acts at the junction between mycolic acid and the PG (21,22). It should be noted here that the mycobacterial cell envelope contains, besides PG, an outer * The work was supported by a rapid grant from the Department of Biotechnology, Government of India.…”

mentioning

confidence: 99%

Molecular Dissection of Phage Endolysin

Pohane

Joshi

Jain

2014

Journal of Biological Chemistry

View full text Add to dashboard Cite

Background: Bacteriophages produce endolysins to hydrolyze host cell wall for their progeny release. Results: Mycobacterium phage endolysin is inactive in E. coli but is active in mycobacteria. Conclusion: An interdomain interaction makes Lysin A inactive in E. coli. Significance: A hydrolase with built-in mechanism attains host specificity. An in-depth study can help in developing strong anti-tuberculosis agents.

show abstract

Genome structure of mycobacteriophage D29: implications for phage evolution 1 1Edited by J. Karn

Cited by 178 publications

References 34 publications

The First Structure of a Mycobacteriophage, the Mycobacterium abscessus subsp. bolletii Phage Araucaria

The First Structure of a Mycobacteriophage, the Mycobacterium abscessus subsp. bolletii Phage Araucaria

Genetic modifications to temperate Enterococcus faecalis phage ϕEf11 that abolish the establishment of lysogeny and sensitivity to repressor, and increase host range and productivity of lytic infection

Molecular Dissection of Phage Endolysin

Contact Info

Product

Resources

About