Genome-Tagged Mice (GTM): Two Sets of Genome-wide Congenic Strains

Iakoubova, Olga A.; Olsson, Christine; Dains, Katherine M.; Ross, David A.; Andalibi, Ali; Lau, Kit; Choi, Jim; Kalcheva, Iveta; Cunanan, Madalyne; Louie, Judi; Nimon, Vitaly; Machrus, M.; Bentley, L. Gordon; Beauheim, Catherine; Silvey, Scott; Cavalcoli, James D.; Lusis, Aldons J.; West, David B.

doi:10.1006/geno.2000.6497

Cited by 71 publications

(68 citation statements)

References 57 publications

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“…This can be achieved by adding more recombinations; i.e., generating inter-and/or backcross panels between B6 and D2 mice. Another important resource that became available only very recently are congenic GTM (Genome Tagged Mice) mice (Iakoubova et al, 2001). GTM mice comprise two sets of lines of mice of B6 background.…”

Section: Qtlmentioning

confidence: 99%

Perchance to dream: Solving the mystery of sleep through genetic analysis

Shaw

Franken

2002

J. Neurobiol.

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Sleep has been identified in all mammals and nonmammalian vertebrates that have been critically evaluated. In addition, sleep-like states have also been identified and described in several invertebrates. Despite this prevalence throughout the animal kingdom, the function of sleep remains a mystery. The completion of several genome sequencing projects has led to the expectation that fundamental aspects of sleep can be elucidated through genetic dissection. Indeed, studies in both the mouse and fly have begun to reveal tantalizing suggestions about the underlying principles that regulate sleep homeostasis. In this article we will review recent studies that have used genetic techniques to evaluate sleep in the fruit fly and the mouse.

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Section: Qtlmentioning

confidence: 99%

Perchance to dream: Solving the mystery of sleep through genetic analysis

Shaw

Franken

2002

J. Neurobiol.

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“…To further investigate if these regions on Chr 1 modulate vocalization to a mild footshock, two strains of B6.D2 Chr 1 mice (Chr 1M and Chr 1D; see Iakoubova et al 2001;see Methods) were assessed for vocalization. Use of these congenic strains confirmed that a region on Chr 1 modulated vocalization to a mild footshock (Two-way ANOVA, strain × sex; main effect of strain, F = 29.35, df (3, 32), P < 0.0001).…”

Section: Resultsmentioning

confidence: 99%

“…Male and female mice from The Jackson Laboratory of the C57BL/6J (B6), A/J (A), DBA/2J (D2), and seven B6.A consomic strains (consomic strains for A Chr 1, 2, 4, 9, 11, 14, and 16; whose official nomenclature is C57BL/6J-Chr # A /NaJ and two B6.D2 congenic strains (termed Genome-tagged lines or GTM; official nomenclature of B6.D2)/LusJ; Iakoubova et al 2001) were used in these experiments. The two B6.D2 congenic lines were targeted to Chr 1 and termed 1M and 1D for medial and distal portions of the chromosome, respectively (between 25-85 cM for 1M and 80-115 for 1D; Iakoubova et al 2001).…”

Section: Subjectsmentioning

confidence: 99%

“…The two B6.D2 congenic lines were targeted to Chr 1 and termed 1M and 1D for medial and distal portions of the chromosome, respectively (between 25-85 cM for 1M and 80-115 for 1D; Iakoubova et al 2001). Consomic mice were originally obtained from Dr. Joe Nadeau or the Jackson Laboratory.…”

Section: Subjectsmentioning

confidence: 99%

“…Several recently developed and powerful genetic tools are now available to investigate this behavioral phenotype in mice. Novel approaches include use of the recently constructed chromosome substitution (Singer et al 2004), consomic (Santos et al 2002), and congenic (Davis et al 2005;Iakoubova et al 2001) mouse strains. These mice have a single chromosome, or segments of a chromosome, from one strain of mouse introgressed into the genetic background of another strain (e.g.…”

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Genetic Mapping of Vocalization to a Series of Increasing Acute Footshocks Using B6.A Consomic and B6.D2 Congenic Mouse Strains

et al. 2008

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Footshock response is used to study a variety of biological functions in mammals including drug self-administration, learning and memory and nociception. However, the genetics underlying variability in footshock sensitivity are not well understood. In the current studies, a panel of B6.A consomic mouse strains, two B6.D2 genome-tagged mouse lines, and the progenitor strains were screened for footshock sensitivity as measured by audible vocalization. It was found that A/J (A) mice and C57BL/6J (B6) mice with an A Chromosome 1 (Chr 1) were less sensitive to footshock compared to B6 animals. Furthermore, the offspring of Chr 1 consomic mice crossed with B6 mice had vocalization levels that were intermediate to A/J and B6 animals. A F2 mapping panel revealed two significant QTLs for footshock vocalization centered around D1Mit490 and D1Mit206 on Chr 1. The role of these Chr 1 loci in footshock sensitivity was confirmed in B6.D2 genome-tagged mouse lines.

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Genetic Modifiers

Threadgill

Hunter²,

Zou

et al. 2004

Mouse Models of Human Cancer

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Cancer is caused by a complex interaction between an individual's intrinsic genetic composition and extrinsic environmental exposures. Cancer modifiers, a special class of cancer genes that accounts for the majority of the intrinsic genetic differences in cancer susceptibility among individuals, contribute to a significant proportion of all human cancers. Modifiers are often normal allelic variants of genes that do not produce abnormal phenotypes alone, but rather “modify” the action of other cancer genes. Frequently, these genes are also called low‐penetrance cancer genes since, individually, they typically have small effects on cancer phenotypes. However, cumulatively, they can have major influences on all stages of cancer development from initiation through response to therapy. Similar to the unique characteristics that distinguish cancer modifiers from high‐penetrance cancer‐causing genes, the methods used to identify these genes are distinct. Because of the low‐penetrance nature of their actions, this class of cancer gene is particularly difficult to detect using human populations. As such, the mouse has become the predominant model to identify cancer modifiers. The unique characteristics, methods for detection and identification of cancer modifiers, and their use in human studies are described in this chapter.

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Genome-Tagged Mice (GTM): Two Sets of Genome-wide Congenic Strains

Cited by 71 publications

References 57 publications

Perchance to dream: Solving the mystery of sleep through genetic analysis

Perchance to dream: Solving the mystery of sleep through genetic analysis

Genetic Mapping of Vocalization to a Series of Increasing Acute Footshocks Using B6.A Consomic and B6.D2 Congenic Mouse Strains

Genetic Modifiers

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