Genome types of adenovirus type 7 isolated in Hiroshima City

Abstract

One hundred thirty-two strains of adenovirus type 7 were isolated in Hiroshima City during the period 1995-1999. About 80% of the viruses were isolated from patients with respiratory illness, and about 70% were isolated from children younger than 6 years old. DNA restriction analysis was performed on 58 of the 132 isolates. Two genome types corresponding to adenovirus 7d and adenovirus 7h were recognized using BamHI. By using an additional 10 restriction endonucleases (BclI, BglI, BglII, BstEII, HindIII, HpaI, SmaI, PstI, PvuII, and SacI), the 58 isolates were classified finally into five genome types according to Wadell and coworkers' nomenclature system: adenovirus 7d2 (54 isolates), adenovirus 7d3 (one isolate), adenovirus 7d4 (one isolate), adenovirus 7d5 (one isolate), and adenovirus 7h (one isolate), among which adenovirus 7d3, 7d4, and 7d5 were new genome types. Our results indicate that the predominant genome type of adenovirus 7 that circulated in Hiroshima City during the period May 1995-November 1999 was adenovirus 7d2.

Complete Genome Sequence of Human Adenovirus Type 7 Associated with Fatal Infant Pneumonia

“…But HAdV7, a member of the B1 subspecies, has been identified in epidemics to be highly virulent and is associated with clinical manifestations of considerable severity, including residual lung damage and fatal outcomes (8–10). This pathogen has been found in outbreaks in the United States and Asia and has become a global concern (11–13). It also poses a special threat for infants and young children as their immune systems are not completely established.…”

Impact of Human Adenovirus Serotype 7 in Hospitalized Children with Severe Fatal Pneumonia in the Philippines

“…Although a higher virulence of HAdV7h was indicated during these outbreaks, there is no virological evidence to indicate increased virulence of HAdV7h. Outside of South America, HAdV7h was first identified in Japan in 1996 and then in the US in 1998 (5,9,23).…”

No evidence of a death-like function for species B1 human adenovirus type 3 E3-9K during A549 cell line infection

“…Recent molecular epidemiology studies have shown that the most prevalent strains of HAdV-B1 isolated from cases of acute respiratory disease encode versions of ORF E3-10.9K that contain truncating or null mutations [10,37,38] suggesting the existence of a selective advantage for loss of this gene among pathogenic HAdV-B1s. Interestingly, the genomes of HAdV-B2s appear to be naturally occurring deletion mutants for this ORF [39-41].…”