Genome-Wide Analysis and Expression of Cyclic Nucleotide–Gated Ion Channel (CNGC) Family Genes under Cold Stress in Mango (Mangifera indica)

Abstract

The ‘king of fruits’ mango (Mangifera indica) is widely cultivated in tropical areas and has been threatened by frequent extreme cold weather. Cyclic nucleotide–gated ion channel (CNGC) genes have an important function in the calcium-mediated development and cold response of plants. However, few CNGC-related studies are reported in mango, regardless of the mango cold stress response. In this study, we identified 43 CNGC genes in mango showing tissue-specific expression patterns. Five MiCNGCs display more than 3-fold gene expression induction in the fruit peel and leaf under cold stress. Among these, MiCNGC9 and MiCNGC13 are significantly upregulated below 6 °C, suggesting their candidate functions under cold stress. Furthermore, cell membrane integrity was damaged at 2 °C in the mango leaf, as shown by the content of malondialdehyde (MDA), and eight MiCNGCs are positively correlated with MDA contents. The high correlation between MiCNGCs and MDA implies MiCNGCs might regulate cell membrane integrity by regulating MDA content. Together, these findings provide a valuable guideline for the functional characterization of CNGC genes and will benefit future studies related to cold stress and calcium transport in mango.

Effects of 10 Dwarfing Interstocks on Cold Resistance of ‘Tianhong 2’ Apple

“…Under low-temperature stress, the degree of cell membrane damage and plant cold resistance can be evaluated by measuring MDA content [26]. The content of MDA in mango increased with the decrease in temperature [27]. In this study, with the decrease in temperature, the MDA content of 'Tianhong 2' apple branches grafted on different interstocks showed a gradual upward trend.…”

Cold-stress induced metabolomic and transcriptomic changes in leaves of three mango varieties with different cold tolerance

“…In fruit peels, cold stress had an effect on carotenoid and flavonoid biosynthesis, phenylpropanoid biosynthesis, and genes related to oxidative stress [ 13 ]. Another study highlighted the potential role of cyclic nucleotide-gated ion channels in cold stress through regulation of malondialdehyde levels [ 14 ]. Using mango transplants, a recent study unraveled the role of salicylic acid (SA) in protecting mango plants from chilling stress [ 15 ].…”