Genome-wide analysis and expression patterns of lipid phospholipid phospholipase gene family in Brassica napus L.

Abstract

Background Lipid phosphate phosphatases (LPP) are critical for regulating the production and degradation of phosphatidic acid (PA), an essential signaling molecule under stress conditions. Thus far, the LPP family genes have not been reported in rapeseed (Brassica napus L.). Results In this study, a genome-wide analysis was carried out to identify LPP family genes in rapeseed that respond to different stress conditions. Eleven BnLPPs genes were identified in the rapeseed genome. Based on phylogenetic and synteny analysis, BnLPPs were classified into four groups (Group I-Group IV). Gene structure and conserved motif analysis showed that similar intron/exon and motifs patterns occur in the same group. By evaluating cis-elements in the promoters, we recognized six hormone- and seven stress-responsive elements. Further, six putative miRNAs were identified targeting three BnLPP genes. Gene ontology analysis disclosed that BnLPP genes were closely associated with phosphatase/hydrolase activity, membrane parts, phosphorus metabolic process, and dephosphorylation. The qRT-PCR based expression profiles of BnLPP genes varied in different tissues/organs. Likewise, several gene expression were significantly up-regulated under NaCl, PEG, cold, ABA, GA, IAA, and KT treatments. Conclusions This is the first report to describe the comprehensive genome-wide analysis of the rapeseed LPP gene family. We identified different phytohormones and abiotic stress-associated genes that could help in enlightening the plant tolerance against phytohormones and abiotic stresses. The findings unlocked new gaps for the functional verification of the BnLPP gene family during stresses, leading to rapeseed improvement.

Mechanistic Insights Into Trehalose-Mediated Cold Stress Tolerance in Rapeseed (Brassica napus L.) Seedlings

“…Rapeseed is a vital oilseed crop worldwide; however, CS significantly affects its growth and production ( He et al, 2021 ; Mehmood et al, 2021 ; Raza, 2021 ; Raza et al, 2021e ; Su et al, 2021b ). Exogenously applied plant growth regulators (phytohormones, osmolytes, neurotransmitters, etc.)…”

Genome-Wide Identification and Expression Profiling of KCS Gene Family in Passion Fruit (Passiflora edulis) Under Fusarium kyushuense and Drought Stress Conditions

“…It has been reported that miRNAs were found to be involved in cuticular wax biosynthesis in Arabidopsis such as trans-acting small interfering RNA (tasiRNA) was involved in CER3 silencing during stem wax production ( Lam et al, 2015 ). Wang Z. et al (2017) and Su et al (2021b) conducted a genome-scale study in Brassica napus and discovered several miRNAs targeting the fatty acid and lipid metabolism enzymes that could regulate the fatty acid synthesis, lipid transport, and metabolism. Later on, Liu et al (2019) proposed that the bna-miR165a-5p ( Brassica napus miRNA) might be involved in the wax biosynthesis process by regulating the putative target BnaA06g40560D gene.…”

Genome-Wide Characterization of Glutathione Peroxidase (GPX) Gene Family in Rapeseed (Brassica napus L.) Revealed Their Role in Multiple Abiotic Stress Response and Hormone Signaling

“…Rapeseed is an allotetraploid crop that practices widespread genome replication and integration activities [49]. However, rapeseed yield is influenced by numerous abiotic pressures, including temperature (cold/heat), high salinity, drought, waterlogging, and heavy metals toxicity [24,27,50]. The generation of ROS in response to normal and stress atmospheres is assessed and scavenged via GPXs, which is considered one of the most effective ROS antioxidant scavenging enzymes [6,9].…”

“…Rapeseed (Brassica napus L.) is the second most significant oilseed crop and holds a complex genome. Various abiotic stresses meaningfully restrict rapeseed production [24][25][26][27]. To our best knowledge, the GPX gene family has not been documented in rapeseed.…”