Genome-wide analysis and expression profiling of PP2C clade D under saline and alkali stresses in wild soybean and Arabidopsis

Chen, Chao; Yu, Youjian; Ding, Xiaodong; Liu, Beidong; Duanmu, Huizi; Zhu, Dan; Sun, Xiaofen; Cao, Lei; Nisa, Zaib-un-; Li, Qiang; Zhu, Yanming

doi:10.1007/s00709-017-1172-2

Cited by 33 publications

(23 citation statements)

References 57 publications

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“…PP2Cs play important roles in various stress signaling pathways. Plant PP2Cs function in response to stresses such as drought, salt, alkali, fungal pathogens (Bhaskara et al, 2012; Chen et al, 2017; Akimoto-Tomiyama et al, 2018) as well as in plant development (Yu et al, 2003; Song et al, 2006; Fuchs et al, 2012; Ren et al, 2018). However, limited research has been conducted on PP2Cs in wheat, and the only report on TaPP2C1 , belonging to group F, is based on its role in resistance to salt stress in transgenic tobacco (Hu W. et al, 2015).…”

Section: Discussionmentioning

confidence: 99%

“…Two TaPP2C homoeologs in group A were unexpectedly up-regulated by heat treatment but not by drought treatment. A previous study demonstrated that almost all members of group D in soybean ( Glycine max ) and Arabidopsis contain SA responsive element, heat stress responsive element (HSE), and MYB binding site involved in drought-inducibility (MBS) in their promoters (Chen et al, 2017). This finding is consistent with our results that genes in group D responded to pathogen infection as well as heat and drought treatments.…”

Section: Discussionmentioning

confidence: 99%

“…Group D comprises nine PP2Cs in Arabidopsis , all of which exhibit different subcellular localization and expression patterns (Tovar-Mendez et al, 2014). Additionally, AtPP2CD5, D6, D7 , and D9 exhibit diverse responses to alkali stress (Chen et al, 2017). Moreover, AtPP2CD2, D5, and D6 proteins negatively regulate SAUR-mediated cell expansion (Ren et al, 2018).…”

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification and Homoeologous Expression Analysis of PP2C Genes in Wheat (Triticum aestivum L.)

Han

Wang

et al. 2019

Front. Genet.

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Plant protein phosphatase 2Cs (PP2Cs) play crucial roles in phytohormone signaling, developmental processes, and both biotic and abiotic stress responses. However, little research has been conducted on the PP2C gene family in hexaploid wheat ( Triticum aestivum L.), which is an important cereal crop. In this study, a genome-wide investigation of TaPP2C gene family was performed. A total of 257 homoeologs of 95 TaPP2C genes were identified, of which 80% of genes had all the three homoeologs across A, B, and D subgenomes. Domain analysis indicated that all the TaPP2C homoeologs harbored the type 2C phosphatase domains. Based on the phylogenetic analysis, TaPP2Cs were divided into 13 groups (A-M) and 4 single branches, which corresponded to the results of gene structure and protein motif analyses. Results of chromosomal location and synteny relationship analysis of TaPP2C homoeologs revealed that known chromosome translocation events and pericentromeric inversions were responsible for the formation of TaPP2C gene family. Expression patterns of TaPP2C homoeologs in various tissues and under diverse stress conditions were analyzed using publicly available RNA-seq data. The results suggested that TaPP2C genes regulate wheat developmental processes and stress responses. Homoeologous expression patterns of TaPP2C triad homoeologs from A, B, and D subgenomes, revealed expression bias within triads under the normal condition, and variability in expression under different stress treatments. Quantitative real-time PCR (qRT-PCR) analysis of eight TaPP2C genes in group A revealed that they were all up-regulated after abscisic acid treatment. Some genes in group A also responded to other phytohormones such as methyl jasmonate and gibberellin. Yeast two-hybrid assays showed that group A TaPP2Cs also interacted with TaSnRK2.1 and TaSnRK2.2 from subclass II, besides with subclass III TaSnRK2s. TaPP2C135 in group A was transformed into Arabidopsis and germination assay revealed that ectopic expression of TaPP2C135 in Arabidopsis enhanced its tolerance to ABA. Overall, these results enhance our understanding of the function of TaPP2Cs in wheat, and provide novel insights into the roles of group A TaPP2Cs. This information will be useful for in-depth functional analysis of TaPP2Cs in future studies and for wheat breeding.

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Section: Discussionmentioning

confidence: 99%

Section: Discussionmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Genome-Wide Identification and Homoeologous Expression Analysis of PP2C Genes in Wheat (Triticum aestivum L.)

Han

Wang

et al. 2019

Front. Genet.

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“…The three-dimensional change of water content in drip irrigation leads to the corresponding three-dimensional movement of saline-alkali. In this way, the movement of saline-alkali has its specific law [28]. after infiltration, the unit is ms cm -1 .…”

Section: Saline-alkali Infiltration Of Drip Irrigation Under Sub-soilmentioning

confidence: 99%

Saline-alkali migration in soda saline soil based on sub-soiling technology

Yuan¹,

Feng²,

Jang³

et al. 2019

Desalination and Water Treatment

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To study the saline-alkali migration law of soda saline-alkali soils based on sub-soiling technology, improve the soil quality of soda saline-alkali agricultural area, and provide scientific basis for the construction of high and stable yield fields of crops in soda saline-alkali soils. Based on the unsaturated soil water movement theory and the convection-dispersion theory of solute transport in porous media, a single-parameter and two-parameter model of saline-alkali transport was constructed to analyze the distribution of saline-alkali concentration in soil solution with the depth of soil layer. Soil samples were taken from four soda saline-alkali soils in different periods under sub-soiling tillage. The experimental data of soil saline-alkali content were obtained, and the law of saline-alkali migration was analyzed. When the depth was 60 cm, the content of saline-alkali in the soil before breaking the plough bottom was 0.4%, and the content of saline-alkali was 0.35% after breaking the plough bottom with sub-soiling technique. It can be seen that sub-soiling technique can promote saline-alkali infiltration and reduce the content of saline-alkali in the planting soil layer. The average water content of the sub-soiled soil was 23.4%, which was significantly higher than that of the subsoiled soil. The soil salinity and alkali content in 100 cm depth layer decreased by 30.8%, and that in 40 cm depth layer decreased by 57%. The salinity and alkali data in different regions showed that the soil salinity and alkali content in the soil decreased by 30.8%. Saline-alkali data from different regions show that due to sub-soiling, salt-alkali in the soil moves upward, salt-alkali in the lower part of crop roots moves outward, and salt-alkali in the soil moves away from the crop root zone; between 0 and 50 cm under the soil, the content of soil salt and alkali is from small to large, forming a desalination area of saline and alkali content; sub-soiling tillage makes 0-30 cm layer soil desalinization rate reach 83.5%, the soil salt removal rate of 0-60 cm layer reached 79.9%; The effect of sub-soiling on soil salinity is less than 1.634% of the initial value. Conclusion: sub-soiling technology can effectively reduce saline alkali content and increase crop yield in soda saline alkali soil.

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“…Also, transgenic studies in maize and Arabidopsis verified that ZmPP2C-A10 function as negative regulator of drought tolerance ( Xiang et al, 2017 ). So far, using bioinformatics analysis, the PP2C gene family has been identified in several species, including Arabidopsis ( Schweighofer et al, 2004 ), rice ( Xue et al, 2008 ), cotton ( Shazadee et al, 2019 ), wheat ( Yu et al, 2019 ), soybean ( Chen et al, 2018 ), and Chinese cabbage ( Khan et al, 2019 ). However, the functional characterization of PP2C genes in cotton remains largely obscure.…”

Section: Introductionmentioning

confidence: 99%

GhHAI2, GhAHG3, and GhABI2 Negatively Regulate Osmotic Stress Tolerance via ABA-Dependent Pathway in Cotton (Gossypium hirsutum L.)

et al. 2022

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The type 2C protein phosphatases (PP2Cs) are well known for their vital roles in plant drought stress responses, but their molecular mechanisms in cotton (Gossypium hirsutum L.) remain largely unknown. Here, we investigated the role of three clade A PP2C genes, namely, GhHAI2, GhAHG3, and GhABI2, in regulating the osmotic stress tolerance in cotton. The transcript levels of GhHAI2, GhAHG3, and GhABI2 were rapidly induced by exogenous abscisic acid (ABA) and polyethylene glycol (PEG) treatment. Silencing of GhHAI2, GhAHG3, and GhABI2 via virus-induced gene silencing (VIGS) improved osmotic tolerance in cotton due to decreased water loss, increase in both relative water content (RWC) and photosynthetic gas exchange, higher antioxidant enzyme activity, and lower malondialdehyde (MDA) content. The root analysis further showed that GhHAI2, GhAHG3, and GhABI2-silenced plants were more responsive to osmotic stress. Yeast two-hybrid (Y2H) and luciferase complementation imaging (LCI) assays further substantiated that GhHAI2, GhAHG3, and GhABI2 interact with the core receptors of ABA signaling, GhPYLs. The expression of several ABA-dependent stress-responsive genes was significantly upregulated in GhHAI2-, GhAHG3-, and GhABI2-silenced plants. Our findings suggest that GhHAI2, GhAHG3, and GhABI2 act as negative regulators in the osmotic stress response in cotton through ABA-mediated signaling.

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Genome-wide analysis and expression profiling of PP2C clade D under saline and alkali stresses in wild soybean and Arabidopsis

Cited by 33 publications

References 57 publications

Genome-Wide Identification and Homoeologous Expression Analysis of PP2C Genes in Wheat (Triticum aestivum L.)

Genome-Wide Identification and Homoeologous Expression Analysis of PP2C Genes in Wheat (Triticum aestivum L.)

Saline-alkali migration in soda saline soil based on sub-soiling technology

GhHAI2, GhAHG3, and GhABI2 Negatively Regulate Osmotic Stress Tolerance via ABA-Dependent Pathway in Cotton (Gossypium hirsutum L.)

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