Genome-wide analysis and identification of light-harvesting chlorophyll a/b binding (LHC) gene family and BSMV-VIGS silencing TaLHC86 reduced salt tolerance in wheat

Chen, Liuping; Yang, Weibing; Liu, Shuqing; Meng, Ying; Zhu, Zhenshuo; Rui, Liang; Cao, Kan; Xie, Yanzhou; Li, Xuejun

doi:10.1016/j.ijbiomac.2023.124930

Cited by 20 publications

(8 citation statements)

References 44 publications

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“…The rate of photosynthesis is known to be influenced by salt stress, with photosystem I chlorophyll ab-binding proteins (LHC) playing a crucial role in this process and being widely implicated in plant stress responses. TaLHC86 has been identified as a beneficial gene for salt tolerance [ 62 ]. In our study, we identified a photosystem I chlorophyll a/b-binding protein 5 (encoded by arahy.U5T9YL ) in the blue2 module of shoot tissue, indicating its involvement in salt tolerance mechanisms.…”

Section: Discussionmentioning

confidence: 99%

Weighted gene co-expression network analysis reveals hub genes regulating response to salt stress in peanut

Wang,

Miao,

Zhang

et al. 2024

BMC Plant Biol

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Peanut (Arachis hypogaea L.) is an important oilseed crop worldwide. However, soil salinization becomes one of the main limiting factors of peanut production. Therefore, developing salt-tolerant varieties and understanding the molecular mechanisms of salt tolerance is important to protect peanut yield in saline areas. In this study, we selected four peanut varieties with contrasting response to salt challenges with T1 and T2 being tolerance and S1 and S2 being susceptible. High-throughput RNA sequencing resulted in more than 314.63 Gb of clean data from 48 samples. We identified 12,057 new genes, 7,971of which have functional annotations. KEGG pathway enrichment analysis of uniquely expressed genes in salt-tolerant peanut revealed that upregulated genes in the root are involved in the MAPK signaling pathway, fatty acid degradation, glycolysis/gluconeogenesis, and upregulated genes in the shoot were involved in plant hormone signal transduction and the MAPK signaling pathway. Na+ content, K+ content, K+/ Na+, and dry mass were measured in root and shoot tissues, and two gene co-expression networks were constructed based on weighted gene co-expression network analysis (WGCNA) in root and shoot. In this study, four key modules that are highly related to peanut salt tolerance in root and shoot were identified, plant hormone signal transduction, phenylpropanoid biosynthesis, starch and sucrose metabolism, flavonoid biosynthesis, carbon metabolism were identified as the key biological processes and metabolic pathways for improving peanut salt tolerance. The hub genes include genes encoding ion transport (such as HAK8, CNGCs, NHX, NCL1) protein, aquaporin protein, CIPK11 (CBL-interacting serine/threonine-protein kinase 11), LEA5 (late embryogenesis abundant protein), POD3 (peroxidase 3), transcription factor, and MAPKKK3. There were some new salt-tolerant genes identified in peanut, including cytochrome P450, vinorine synthase, sugar transport protein 13, NPF 4.5, IAA14, zinc finger CCCH domain-containing protein 62, beta-amylase, fatty acyl-CoA reductase 3, MLO-like protein 6, G-type lectin S-receptor-like serine/threonine-protein kinase, and kinesin-like protein KIN-7B. The identification of key modules, biological pathways, and hub genes in this study enhances our understanding of the molecular mechanisms underlying salt tolerance in peanuts. This knowledge lays a theoretical foundation for improving and innovating salt-tolerant peanut germplasm.

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Section: Discussionmentioning

confidence: 99%

Weighted gene co-expression network analysis reveals hub genes regulating response to salt stress in peanut

Wang,

Miao,

Zhang

et al. 2024

BMC Plant Biol

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“…AOS may alleviate stress damage by modulating light-trapping proteins in rice seedlings exposed to salt stress. In wheat, LHCs are highly expressed in chloroplast tissues, and overexpression often improves tolerance to stress [ 33 ]. However, in this experiment, the AOS downregulated the light-harvesting protein-related genes.…”

Section: Discussionmentioning

confidence: 99%

Combined transcriptomic and metabolomic analysis of alginate oligosaccharides alleviating salt stress in rice seedlings

Du,

Liu,

Feng

et al. 2023

BMC Plant Biol

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Background Salt stress is one of the key factors limiting rice production. Alginate oligosaccharides (AOS) enhance plant stress resistance. However, the molecular mechanism underlying salt tolerance in rice induced by AOS remains unclear. FL478, which is a salt-tolerant indica recombinant inbred line and IR29, a salt-sensitive rice cultivar, were used to comprehensively analyze the effects of AOS sprayed on leaves in terms of transcriptomic and metabolite profiles of rice seedlings under salt stress. Results In this experiment, exogenous application of AOS increased SOD, CAT and APX activities, as well as GSH and ASA levels to reduce the damage to leaf membrane, increased rice stem diameter, the number of root tips, aboveground and subterranean biomass, and improved rice salt tolerance. Comparative transcriptomic analyses showed that the regulation of AOS combined with salt treatment induced the differential expression of 305 and 1030 genes in FL478 and IR29. The expressed genes enriched in KEGG pathway analysis were associated with antioxidant levels, photosynthesis, cell wall synthesis, and signal transduction. The genes associated with light-trapping proteins and RLCK receptor cytoplasmic kinases, including CBA, LHCB, and Lhcp genes, were fregulated in response to salt stress. Treatment with AOS combined with salt induced the differential expression of 22 and 50 metabolites in FL478 and IR29. These metabolites were mainly related to the metabolism of amino and nucleotide sugars, tryptophan, histidine, and β -alanine. The abundance of metabolites associated with antioxidant activity, such as 6-hydroxymelatonin, wedelolactone and L-histidine increased significantly. Combined transcriptomic and metabolomic analyses revealed that dehydroascorbic acid in the glutathione and ascorbic acid cycles plays a vital role in salt tolerance mediated by AOS. Conclusion AOS activate signal transduction, regulate photosynthesis, cell wall formation, and multiple antioxidant pathways in response to salt stress. This study provides a molecular basis for the alleviation of salt stress-induced damage by AOS in rice.

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“…9A˜D). In particular, chlorophyll a-b binding proteins (LHC), which have a major regulatory role in the light-harvesting function (Jansson 1994;Chen et al, 2023), were significantly upregulated in the low light transmittance group (Fig. 9D).…”

Section: Effects Of Different Light Transmittance On the Photosynthet...mentioning

confidence: 99%

Mechanism of rapid adaptive responses of macrophytes Vallisneria denseserrulata (Makino) to varying visible light transmittance: A mesocosm study

Xia,

Chen,

Chen

et al. 2024

Preprint

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Light transmittance is a closely related and inseparable key environmental limiting factor shaping the presence and distribution of macrophytes in aquatic environments. However, little is known about the responses of the morphology and photosynthetic capacity of macrophytes to different light conditions. Here, we conducted a short-term mesocosm experiment with Vallisneria denseserrulata as subjects, exposing them to the light transmittance of 10%, 20%, 30%, 60%, and 100%. Plant growth indicators and photosynthesis-related indicators were monitored during the 28-day experiment. The results showed that V. denseserrulata responded rapidly to changes in the light environment. Under high light transmittance conditions, V. denseserrulata rapidly expanded to obtain more resources. In low light transmittance conditions, V. denseserrulata mainly maintained its growth, rarely forming ramet, and grew longer leaves and larger leaf areas to improve light acquisition ability. There were 158, 47, 192, and 554 differentially expressed genes (DEGs) were identified in the pairwise comparison of 10%VS100%, 20%VS100%, 30%VS100%, and 60%VS100%, respectively. Gene Ontology (GO) and Kyoto Encyclopedia of Genes and Genomes (KEGG) analysis suggested that the DEGs were mainly involved in “pigment biosynthetic” and “photosynthesis”. Furthermore, genes involved in the photosynthesis pathway obtained different expression levels in V. denseserrulata between different treatments. The lower the light transmittance, the higher the expression of genes involved in photosynthesis in V. denseserrulata. Therefore, macrophytes have strong plasticity to maintain growth in stressful environments. Synthesis: V. denseserrulata exhibits strong plasticity in morphology, cytochrome production, and photosynthetic pathway regulation to maintain its growth in low-light environments. However, our results also indicated that the degraded underwater light climate surely results in a decreased macrophyte community. These results help elucidate the degradation process of submerged macrophytes in turbid lakes and guide the restoration of aquatic plants in eutrophic lakes.

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Genome-wide analysis and identification of light-harvesting chlorophyll a/b binding (LHC) gene family and BSMV-VIGS silencing TaLHC86 reduced salt tolerance in wheat

Cited by 20 publications

References 44 publications

Weighted gene co-expression network analysis reveals hub genes regulating response to salt stress in peanut

Weighted gene co-expression network analysis reveals hub genes regulating response to salt stress in peanut

Combined transcriptomic and metabolomic analysis of alginate oligosaccharides alleviating salt stress in rice seedlings

Mechanism of rapid adaptive responses of macrophytes Vallisneria denseserrulata (Makino) to varying visible light transmittance: A mesocosm study

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