Genome-wide analysis and transcriptional reprogrammings of MYB superfamily revealed positive insights into abiotic stress responses and anthocyanin accumulation in Carthamus tinctorius L.

Hong, Yingqi; Ahmad, Naveed; Zhang, Jianyi; Lv, Yuanda; Zhang, Xinyue; Ma, Xintong; Liu, Xiuming; Yao, Na

doi:10.1007/s00438-021-01839-1

Cited by 25 publications

(17 citation statements)

References 90 publications

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“…Gene function can be reflected, to some extent, in the expression patterns of the gene [ 43 ]. NsMYB1 had a higher transcription level in fruit and a lower expression level in root and leaf tissues, but it was hardly expressed in stem tissue [ 44 ].…”

Section: Discussionmentioning

confidence: 99%

IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco

Wang

Lei

et al. 2022

Genes

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The MYB (v-myb avian myeloblastosis viral oncogene homolog) transcription factor family plays an important role in plant growth, development, and response to biotic and abiotic stresses. However, the gene functions of MYB transcription factors in sweet potato (Ipomoea batatas (L.) Lam) have not been elucidated. In this study, an MYB transcription factor gene, IbMYB308, was identified and isolated from sweet potato. Multiple sequence alignment showed that IbMYB308 is a typical R2R3-MYB transcription factor. Further, quantitative real-time PCR (qRT-PCR) analysis revealed that IbMYB308 was expressed in root, stem, and, especially, leaf tissues. Moreover, it showed that IbMYB308 had a tissue-specific profile. The experiment also showed that the expression of IbMYB308 was induced by different abiotic stresses (20% PEG-6000, 200 mM NaCl, and 20% H2O2). After a 200 mM NaCl treatment, the expression of several stress-related genes (SOD, POD, APX, and P5CS) was upregulation in transgenic plants, and the CAT activity, POD activity, proline content, and protein content in transgenic tobacco had increased, while MDA content had decreased. In conclusion, this study demonstrated that IbMYB308 could improve salt stress tolerance in transgenic tobacco. These findings lay a foundation for future studies on the R2R3-MYB gene family of sweet potato and suggest that IbMYB308 could potentially be used as an important positive factor in transgenic plant breeding to improve salt stress tolerance in sweet potato plants.

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Section: Discussionmentioning

confidence: 99%

IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco

Wang

Lei

et al. 2022

Genes

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“…2B), we found many avonoid metabolites including taxifolin, isoquercitrin, delphinidin-3-Oglucoside, L-epicatechin, chalconaringenin, luteolin, naringenin, hesperetin, rutin, chrysin, hesperetin 7-Oglucoside, kaempferol, cianidanol, setin and peonidin-3-glucoside were signi cantly upregulated as well as N-((-)-jasmonoyl)-S-isoleucine and kaurenoic acid belonging to phytohormone and diterpenoids respectively in M1B7. Increasing evidence supported that accumulation of avonoids contributed to deepen the color of fruits or leaves [21,27]. In the contrast, we noticed that multiple phytohormones including gibberellin, salicylic acid and abscisic acid as well as phenylalanine compounds containing phenylalanine isomerides and phenethylamine were marked enriched in N1B7.…”

Section: Resultsmentioning

confidence: 59%

Integrated metabolome and transcriptome reveals the mechanism of the flower coloration in cashew (Anacardium occidentale Linn.)

Zhongrun

Huang

Xiao

et al. 2022

Preprint

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Background Cashew is a widely attractive food in the world with high nutritional value for human health. The quality of cashew is closely associated with the flower coloration. However, the underlying mechanism of flower coloration of cashew was poorly understood. Here, we identified two cashew cultivars with variable flower coloration. Then, integrated analysis of metabolome and transcriptome was performed to explore the potential mechanisms. Results The results showed that 8 flavonoid compounds were markedly accumulated in red flower cashew, including taxifolin, procyanidin B2, delphinidin-3-O-glucoside, peonidin-3-glucoside, L-epicatechin, chalconaringenin, luteolin, naringenin and chrysin. In particular, delphinidin-3-O-glucoside, peonidin-3-glucoside and procyanidin B2 were key metabolites that promoted the formation of red flower coloration. Transcriptome analysis revealed that genes involved in flavonoid biosynthesis were also varied among these two cultivars. The flavonoid biosynthesis direction was reprogrammed to synthesize delphinidin-3-O-glucoside, peonidin-3-glucoside and procyanidin B2. Typically, four core genes encoding anthocyanin synthesis were also highly expressed in cashew with red flower, including CFI (TRINITY_DN4346_c0_g1), CHS2 (TRINITY_DN16129_c0_g1), CHS1 (TRINITY_DN2623_c0_g1) and F3H (TRINITY_DN8780_c0_g1). Conclusions This study sheds light on flavonoid metabolic pathways and candidate genes varied in two cashew cultivars and lays a foundation for the breeding programs of cashew.

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“…The growth conditions of safflower (Daytime temperature 26°C, night temperature 20°C, relative humidity 80%, light 16 h, darkness 8 h), Nicotiana benthamiana (the temperature is 25°C, the light time is 16 h/the dark light time is 8 h), and Arabidopsis (grow in a greenhouse at 22°C, light time is 16 h/dark time is 8 h) used in this study were as described in our earlier studies (Hong et al, 2022a, 2022b). The Arabidopsis mutants bhlh41 (SALK_085759C), myb63 (SALK_035416C), and wd40‐6 (SALK_068763C) were purchased from AraShare (http://www.arashare.cn/).…”

Section: Methodsmentioning

confidence: 99%

“…Expression analysis was calculated using the formula for

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. The primers for qRT‐PCR analysis used in this study are shown in Table S1 (Hong et al, 2022a).…”

Section: Methodsmentioning

confidence: 99%

The safflower MBW complex regulates HYSA accumulation through degradation by the E3 ligase CtBB1

Hong

Zhang

et al. 2023

JIPB

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The regulatory mechanism of the MBW (MYB-bHLH-WD40) complex in safflower (Carthamus tinctorius) remains unclear. In the present study, we show that the separate overexpression of the genes CtbHLH41, CtMYB63, and CtWD40-6 in Arabidopsis thaliana increased anthocyanin and procyanidin contents in the transgenic plants and partially rescued the trichome reduction phenotype of the corresponding bhlh41, myb63, and wd40-6 single mutants. Overexpression of CtbHLH41, CtMYB63, or CtWD40-6 in safflower significantly increased the content of the natural pigment hydroxysafflor yellow A (HYSA) and negatively regulated safflower petal size. Yeast-two-hybrid, functional, and genetic assays demonstrated that the safflower E3 ligase CtBB1 (BIG BROTHER 1) can ubiquitinate CtbHLH41, marking it for degradation through the 26S proteasome and negatively regulating flavonoid accumulation. CtMYB63/CtWD40-6 enhanced the transcriptional activity of CtbHLH41 on the CtDFR (dihydroflavonol 4-reductase) promoter. We propose that the MBW-CtBB1 regulatory module may play an important role in coordinating HYSA accumulation with other response mechanisms.

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Genome-wide analysis and transcriptional reprogrammings of MYB superfamily revealed positive insights into abiotic stress responses and anthocyanin accumulation in Carthamus tinctorius L.

Cited by 25 publications

References 90 publications

IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco

IbMYB308, a Sweet Potato R2R3-MYB Gene, Improves Salt Stress Tolerance in Transgenic Tobacco

Integrated metabolome and transcriptome reveals the mechanism of the flower coloration in cashew (Anacardium occidentale Linn.)

The safflower MBW complex regulates HYSA accumulation through degradation by the E3 ligase CtBB1

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