Genome-Wide Analysis of Auxin Receptor Family Genes in Brassica juncea var. tumida

Cai, Zhaoming; Zeng, De-Er; Liao, Jingjing; Chen, Cheng; Sahito, Zulfiqar Ali; Xiang, Meiqin; Fu, Min; Chen, Yuanqing; Wang, Diandong

doi:10.3390/genes10020165

Cited by 22 publications

(16 citation statements)

References 49 publications

(64 reference statements)

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“…It has been stated that up to 87% amino acid identity exists in the well‐genome annotated plant species Arabidopsis thaliana , whereas 98% was reported in AFB of Brassica juncea var. tumida 9,35,36 . Hence, the present study evaluated single nucleotide polymorphisms for only TIR1 and AFB genes.…”

Section: Resultsmentioning

confidence: 99%

Non‐target‐site resistance mechanism of barnyardgrass [Echinochloa crus‐galli (L.) P. Beauv.] to florpyrauxifen‐benzyl

Hwang

Norsworthy

González-Torralva

et al. 2021

Pest Management Science

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BACKGROUND Florpyrauxifen‐benzyl (FPB) is an arylpicolinate herbicide (Group IV) for barnyardgrass control in rice. One susceptible (Sus) and three putative FPB‐resistant (R1, R2, and R3) barnyardgrass biotypes were selected based on resistant/susceptible (R/S) ratios obtained from dose–response tests and used to investigate the potential resistance mechanisms. RESULTS Based on visual control results, the R/S ratios of barnyardgrass biotypes R1, R2, and R3 were 60‐, 33‐, and 16‐fold greater than the Sus standard, respectively. Sequencing results of TIR1 and AFB genes in the tested barnyardgrass revealed no difference between Sus and R barnyardgrass biotypes. Absorption of [14C]‐FPB in Sus barnyardgrass increased over time and reached 90%, which was >10 percentage points greater than that in R biotypes. The [14C]‐FPB absorption in all R barnyardgrass equilibrated after 48 h. For both Sus and R barnyardgrass, most [14C]‐FPB absorbed was present in the treated leaf (79.8–88.8%), followed by untreated aboveground (9.5–18.6%) and belowground tissues (1.3–2.2%). No differences in translocation were observed. Differences between Sus and R barnyardgrass biotypes were found for FPB metabolism. Production of the active metabolite, florpyrauxifen‐acid, was greater in Sus barnyardgrass (21.5–52.1%) than in R barnyardgrass (5.5–34.9%). CONCLUSION In conclusion, reductions in FPB absorption and florpyrauxifen‐acid production may contribute to the inability to control barnyardgrass with FPB. © 2021 Society of Chemical Industry.

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Section: Resultsmentioning

confidence: 99%

Non‐target‐site resistance mechanism of barnyardgrass [Echinochloa crus‐galli (L.) P. Beauv.] to florpyrauxifen‐benzyl

Hwang

Norsworthy

González-Torralva

et al. 2021

Pest Management Science

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“…Tumorous stem mustard is an economically important food crop; however, its yield is affected by many abiotic stresses ( Cai et al, 2019 ; Li et al, 2019 ). As one of the largest transcription factor families in plant species, the NAC family plays an important role in plant growth, development, and biotic and abiotic stress responses ( Seo & Park, 2010 ; Nakashima et al, 2012 ).…”

Section: Discussionmentioning

confidence: 99%

Genome-wide identification and characterization of NAC genes in Brassica juncea var. tumida

Jiang

Sun

Wang

et al. 2021

PeerJ

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Background NAC (NAM, ATAF1/2, and CUC2) transcription factors play an important role in plant growth and development. However, in tumorous stem mustard (Brassica juncea var. tumida), one of the economically important crops cultivated in southwest China and some southeast Asian countries, reports on the identification of NAC family genes are lacking. In this study, we conducted a genome-wide investigation of the NAC family genes in B. juncea var. tumida, based on its recently published genome sequence data. Methods The NAC genes were identified in B. juncea var. tumida using the bioinformatics approach on the whole genome level. Additionally, the expression of BjuNAC genes was analyzed under high- and low-temperature stresses by quantitative real-time PCR (qRT-PCR). Results A total of 300 BjuNAC genes were identified, of which 278 were mapped to specific chromosomes. Phylogenetic analysis of B. juncea var. tumida, Brassica rapa, Brassica nigra, rice and Arabidopsis thaliana NAC proteins revealed that all NAC genes were divided into 18 subgroups. Furthermore, gene structure analysis showed that most of the NAC genes contained two or three exons. Conserved motif analysis revealed that BjuNAC genes contain a conserved NAM domain. Additionally, qRT-PCR data indicated that thirteen BjuNAC genes with a varying degree of up-regulation during high-temperature stress. Conversely, four BjuNAC genes (BjuNAC006, BjuNAC083, BjuNAC170 and BjuNAC223) were up-regulated and two BjuNAC genes (BjuNAC074 and BjuNAC295) down-regulated under low temperature, respectively. Together, the results of this study provide a strong foundation for future investigation of the biological function of NAC genes in B. juncea var. tumida.

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“…acephala) resulted in the reduction of downy mildew symptoms caused by Peronospora brassicae, indicating that this gene may develop a network with regulatory genes to offer resistance to downy mildew disease [ 53 ]. In contrast, BjuTIR1/AFB family genes are the auxin receptor genes that were found to be greatly induced by P. brassicae treatment, indicating that upregulation of auxin receptor genes may positively regulate clubroot formation [ 54 ]. In this study, induced expression levels of BrDCK1 gene by 5.3- and 2.5-fold in Seosan-inoculated plants compared to mock-treated plants at 3 dpi and 14 dpi, respectively, indicate that this gene has importance in both primary infection and cortex infection ( Figure 4 and Figure 8 ).…”

Section: Discussionmentioning

confidence: 99%

Expression and Role of Biosynthetic, Transporter, Receptor, and Responsive Genes for Auxin Signaling during Clubroot Disease Development

Robin

Saha

Laila

et al. 2020

IJMS

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Auxins play a pivotal role in clubroot development caused by the obligate biotroph Plasmodiophora brassicae. In this study, we investigated the pattern of expression of 23 genes related to auxin biosynthesis, reception, and transport in Chinese cabbage (Brassica rapa) after inoculation with P. brassicae. The predicted proteins identified, based on the 23 selected auxin-related genes, were from protein kinase, receptor kinase, auxin responsive, auxin efflux carrier, transcriptional regulator, and the auxin-repressed protein family. These proteins differed in amino acids residue, molecular weights, isoelectric points, chromosomal location, and subcellular localization. Leaf and root tissues showed dynamic and organ-specific variation in expression of auxin-related genes. The BrGH3.3 gene, involved in auxin signaling, exhibited 84.4-fold increase in expression in root tissues compared to leaf tissues as an average of all samples. This gene accounted for 4.8-, 2.6-, and 5.1-fold higher expression at 3, 14, and 28 days post inoculation (dpi) in the inoculated root tissues compared to mock-treated roots. BrNIT1, an auxin signaling gene, and BrPIN1, an auxin transporter, were remarkably induced during both cortex infection at 14 dpi and gall formation at 28 dpi. BrDCK1, an auxin receptor, was upregulated during cortex infection at 14 dpi. The BrLAX1 gene, associated with root hair development, was induced at 1 dpi in infected roots, indicating its importance in primary infection. More interestingly, a significantly higher expression of BrARP1, an auxin-repressed gene, at both the primary and secondary phases of infection indicated a dynamic response of the host plant towards its resistance against P. brassicae. The results of this study improve our current understanding of the role of auxin-related genes in clubroot disease development.

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Genome-Wide Analysis of Auxin Receptor Family Genes in Brassica juncea var. tumida

Cited by 22 publications

References 49 publications

Non‐target‐site resistance mechanism of barnyardgrass [Echinochloa crus‐galli (L.) P. Beauv.] to florpyrauxifen‐benzyl

Non‐target‐site resistance mechanism of barnyardgrass [Echinochloa crus‐galli (L.) P. Beauv.] to florpyrauxifen‐benzyl

Genome-wide identification and characterization of NAC genes in Brassica juncea var. tumida

Expression and Role of Biosynthetic, Transporter, Receptor, and Responsive Genes for Auxin Signaling during Clubroot Disease Development

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