Genome-Wide Analysis of Dynamin Gene Family in cassava (Manihot esculenta Crantz) and Transcriptional Regulation of Family Members ARC5 in Hormonal Treatments

Abstract

The Dynamin gene family play a significance role in many physiological processes, especially ARC5 (Accumulation and replication of chloroplasts 5) in the process of plastid division. We performed a genome-wide analysis of the cassava Dynamin family based on the published cassava genome sequence and identified ARC5. 23 cassava Dynamins (MeDynamins) were identified and renamed. 23 MeDynamins were further divided into five major groups based on their structural and phylogenetic characteristics. The segmental duplication events have a significant impact on the expansion of MeDynamins. ARC5 expression analysis showed that there were differences between leaves and roots of cassava at different developmental stages. The tissue-specific expression analysis of the MeDynamins showed that most of MeDynamins were expressed in stem apical meristem and embryogenesis, whereas ARC5 was mainly expressed in leaves. The processing of IAA (Indole-3-acetic Acid) and MeJA (Methyl Jasmonate) verified the prediction results of cis-elements, and ACR5 was closely related to plant growth and positively correlated. It also indicated that high concentrations of MeJA treatment caused the cassava defense mechanism to function in advance. In conclusion, these findings provide basic insights for functional validation of the ARC5 genes in exogenous hormonal treatments.

Genome-Wide Identification of the WRKY Gene Family and Functional Characterization of CpWRKY5 in Cucurbita pepo

“…Domain loss appears to be common in monocots such as rice and maize, and the loss of the WRKY domain is among the divergent forces of WRKY gene family expansion [35][36][37]. In C. pepo, the C-terminal of Group I CpWKRY55 lacked a conserved heptapeptide sequence, and the CpWRKY39 C-terminal demonstrated the loss of the zinc finger structure.…”

Transcriptome and Co-Expression Network Analysis Reveals the Molecular Mechanism of Rice Root Systems in Response to Low-Nitrogen Conditions

“…Some studies have mined and identified many functional genes by constructing a co-expression regulatory network [ 31 , 84 , 85 ]. Additionally, among the 43 candidate genes, DAO , which encodes a growth-hormone-oxidizing dioxygenase, was able to rapidly respond to nitrogen stress [ 86 ]; OsSultr1;1, the sulfate transporter protein, was regulated by nitrogen in a previous study [ 87 ]; Os08g0533300 was presumed to be ACR5 (accumulation and replication of chloroplasts 5), which has been significantly and positively correlated with plant growth [ 88 ]; and OsWNK6 may be functionally similar to OsWNK9 , which negatively regulated root elongation [ 89 , 90 ]. These results suggest that these 43 candidate genes may participate in the regulation of the nitrogen uptake and utilization in rice and improve the adaptability of rice to low-nitrogen conditions.…”

Genome-Wide Analysis of Tubulin Gene Family in Cassava and Expression of Family Member FtsZ2-1 during Various Stress

“…[ 40 ]. Spraying exogenous hormones can significantly affect the growth and development of cassava; for instance, a high concentration of MeJA could induce the cassava defense mechanism to play a role in advance [ 41 , 42 , 43 ]. The application of auxin can stimulate the growth of cassava stem and bud, and tubulins play an important role in this process [ 44 , 45 , 46 ].…”