Genome-Wide Analysis of Known and Potential Tetraspanins in Entamoeba histolytica

Tomii, Kentaro; Santos, Herbert J.; Nozaki, Tomoyoshi

doi:10.3390/genes10110885

Cited by 14 publications

(14 citation statements)

References 51 publications

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“…However, it is important to note that TSPs have been identified in some, but not all protozoan parasite genomes. Specifically, 17 TSPs‐like sequences were found in E. histolytica (Tomii, Santos, & Nozaki, 2019), 1 in Leishmania spp., 3 in T. cruzi and 2 in T. brucei , while no TSPs‐like sequences were found in Giardia spp ., Plasmodium spp ., Toxoplasma gondii and Cryptosporidium parvum (our HMMer search using Pfam PF00335). Therefore, TSPs proteins may not be proposed as general markers in EVs from protozoan parasites.…”

Section: Trichomonas Vaginalismentioning

confidence: 99%

“…Besides the presence of some possible contaminants, significant overlap with orthologs of the top 100 known mammalian exosome markers in Exocarta and an enrichment of proteins required for vesicle membrane production was detected in the Entamoeba EV proteome (Sharma et al, 2020) suggesting that enrichment in EVs was successful. Surprisingly, although a recent bioinformatics analysis identified at least 17 putative tetraspanin proteins in the E. histolytica genome (Tomii et al, 2019), the TSP family proteins were absent from the amoebic EV proteome list (Sharma et al, 2020). Similarly, none of these proteins were detected in the surface proteome (Biller et al, 2014) and localisation experiments that detected EhTSP1, EhTSP2, EhTSP8, EhTSP9, EhTSP12, EhTSP13, EhTSP15 in vesicular compartments in the cytosol, but not on the cell surface (Tomii et al, 2019).…”

Section: Entamoeba Histolyticamentioning

confidence: 99%

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Extracellular vesicles released by anaerobic protozoan parasites: Current situation

Nievas

Lizarraga

Salas

et al. 2020

Cellular Microbiology

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Extracellular vesicles (EVs) have emerged as a ubiquitous mechanism for transferring information between cells and organisms across all three kingdoms of life. Parasitic unicellular eukaryotes use EVs as vehicles for intercellular communication and host manipulation. Pathogenic protozoans are able to modulate the immune system of the host and establish infection by transferring a wide range of molecules contained in different types of EVs. In addition to effects on the host, EVs are able to transfer virulence factors, drug-resistance genes and differentiation factors between parasites. In this review we cover the current knowledge on EVs from anaerobic or microaerophilic extracellular protozoan parasites, including Trichomonas vaginalis, Tritrichomonas foetus, Giardia intestinalis and Entamoeba histolytica, with a focus on their potential role in the process of infection. The role of EVs in host: parasite communication adds a new level of complexity to our understanding of parasite biology, and may be a key to understand the complexity behind their mechanism of pathogenesis.

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Section: Trichomonas Vaginalismentioning

confidence: 99%

Section: Entamoeba Histolyticamentioning

confidence: 99%

Extracellular vesicles released by anaerobic protozoan parasites: Current situation

Nievas

Lizarraga

Salas

et al. 2020

Cellular Microbiology

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“…Tetraspanins are transmembrane proteins previously described as potential vaccine candidates for other helminth infections and are also found in the membranes of the tegument and extracellular vesicles of O. viverrini [51]. Their function is associated with virulence and pathogenesis in some organism [52]. A wide variety of molecules have been found to be released by E. granulosus, including tetraspanin proteins, channel proteins that transport lipids, and nucleic acids [53,54].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic Features of Echinococcus granulosus Protoscolex during the Encystation Process

Fan

et al. 2020

Korean J Parasitol

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Cystic echinococcosis (CE) is a zoonotic infection caused by Echinococcus granulosus larvae. It seriously affects the development of animal husbandry and endangers human health. Due to a poor understanding of the cystic fluid formation pathway, there is currently a lack of innovative methods for the prevention and treatment of CE. In this study, the protoscoleces (PSCs) in the encystation process were analyzed by high-throughput RNA sequencing. A total of 32,401 transcripts and 14,903 cDNAs revealed numbers of new genes and transcripts, stage-specific genes, and differently expressed genes. Genes encoding proteins involved in signaling pathways, such as putative G-protein coupled receptor, tyrosine kinases, and serine/threonine protein kinase, were predominantly up-regulated during the encystation process. Antioxidant enzymes included cytochrome c oxidase, thioredoxin glutathione, and glutathione peroxidase were a high expression level. Intriguingly, KEGG enrichment suggested that differentially up-regulated genes involved in the vasopressin-regulated water reabsorption metabolic pathway may play important roles in the transport of proteins, carbohydrates, and other substances. These results provide valuable information on the mechanism of cystic fluid production during the encystation process, and provide a basis for further studies on the molecular mechanisms of growth and development of PSCs.

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“…Furthermore, tetraspanins are transmembrane proteins previously described as potential vaccine candidates for other helminth infections and are also found in the membranes of the tegument and extracellular vesicles of O. viverrini [56]. In some organisms, their role is associated with virulence and pathogenesis [57]. A wide variety of molecules have been found to be released by E. granulosus, such as tetraspanin proteins, channel protein that transport lipids, and nucleic acids [58,59].…”

Section: Discussionmentioning

confidence: 99%

Transcriptomic analysis of the Echinococcus granulosus protoscolex in the encystation process

Fan¹,

Wu²,

Li³

et al. 2020

Preprint

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Back ground: Echinococcosis (CE) is a zoonosis and in humans it occurs as a result of infection by the larva of Echinococcus granulosus . CE is seriously affects the development of animal husbandry and endangers human health. Due to the lack of in-depth understanding of the cystic fluid formation pathway, prevention and treatment of CE have been lack of innovative methods. Result: High throughput RNA-sequencing (RNA-seq) of protoscoleces (PSCs) in the encystation process of total three biological replicates for each period on 0d, 10d, 20d, 40d and 80d were analyzed. The results demonstrated, a total of 32,401 transcripts and 14,903 genes, including numbers new genes, new transcript, stage-specific genes and differently expression genes (DEGs). Genes encoding proteins involved in several signaling pathways, such as putative G-protein coupled receptor (GPCR), tyrosine kinases and serine/threonine protein kinase were predominantly up-regulated during encystation process of PSCs. Moreover, three major antioxidant proteins of PSCs were identified, and these proteins demonstrated have a high expression level, including cytochrome c oxidase, thioredoxin glutathione, and glutathione peroxidase. Intriguingly, The Kyoto Encyclopedia of Genes and Genomes (KEGG) enrichment analysis suggested that up-regulated DEGs involved in the vasopressin-regulated water reabsorption metabolic pathway might play important roles in the protein, carbohydrate, and other substances transport. Conclusions: The present study carried out the transcriptomic analysis of the encystation process of E. granulosus PCSs, which provide valuable information for the mechanism of cystic fluid formation during the encystation process. These results provide a basis and reference for further studies for investigation of the molecular mechanisms involved in PSC growth and development. Keywords: Echinococcus granulosus , Encystation process, Differentially expressed genes, Protoscolex, RNA-seq

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Genome-Wide Analysis of Known and Potential Tetraspanins in Entamoeba histolytica

Cited by 14 publications

References 51 publications

Extracellular vesicles released by anaerobic protozoan parasites: Current situation

Extracellular vesicles released by anaerobic protozoan parasites: Current situation

Transcriptomic Features of Echinococcus granulosus Protoscolex during the Encystation Process

Transcriptomic analysis of the Echinococcus granulosus protoscolex in the encystation process

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